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Literature summary for 2.1.2.9 extracted from
- N-formylmethionyl-tRNA of wheat chloroplasts. Its synthesis by a wheat transformylase (1971), Biochemistry, 10, 889-894.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Triticum aestivum | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates, maximum activity at 5 mM Mg2+ and 20 mM KCl | Triticum aestivum |  |
| Mg2+ | requirement | Triticum aestivum | |
| Mg2+ | maximum activity at 5 mM Mg2+ and 20 mM KCl | Triticum aestivum | |
| Na+ | activates, can replace K+ | Triticum aestivum | |
| NH4+ | activates, can replace K+ | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
gel filtration | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Triticum aestivum | component of a protein chain initiating system in chloroplasts | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
wheat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| germ | - |
Triticum aestivum | - |
| leaf | higher specific activity in isolated chloroplasts than in the remainder of the leaf extract | Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Triticum aestivum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, longer than 4 months, stable | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from wheat germ and leaf | Triticum aestivum | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from E. coli | Triticum aestivum | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | component of a protein chain initiating system in chloroplasts | Triticum aestivum | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
| additional information | no formylation of cytoplasmic initiating Met-tRNAi | Triticum aestivum | ? | - |
? | |
| additional information | no formylation of methionyl-tRNAmMet | Triticum aestivum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Triticum aestivum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | good activity between | Triticum aestivum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 10-formyltetrahydrofolate | - |
Triticum aestivum | |
| tetrahydrofolate | - |
Triticum aestivum | |
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Release 2023.1 (January 2023)
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