Cloned (Comment) | Organism |
---|---|
wild-type and mutant fmt gene encoding MTF is cloned, overexpression of wild-type enzyme in Escherichia coli Novablue | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystal structure of enzyme complexed with formylmethionyl-tRNAfMet | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A55T | no suppressor mutation | Escherichia coli |
G178D | suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, it compensates for lysine that is attached to the tRNA, G178D, G178K and G178L are progressively weaker suppressors than G178E | Escherichia coli |
G178E | suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors | Escherichia coli |
G178K | suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E | Escherichia coli |
G178L | suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00051 | - |
L-methionyl-tRNAfMet | wild-type enzyme | Escherichia coli | |
0.00072 | - |
L-methionyl-tRNAfMet | G178E mutant enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli JM109 | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli CA274 | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
strains DH5Alpha, Novablue(DE3) pLysS | - |
Escherichia coli CA274 | - |
- |
- |
Escherichia coli JM109 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
14fold purification of recombinant wild-type enzyme, overexpressed in Escherichia coli Novablue, purification of His-tagged G178E mutant enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + L-methionyl-tRNAfMet | - |
Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet | - |
Escherichia coli JM109 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet | - |
Escherichia coli CA274 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specificity is restricted to methionyl-tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specificity is restricted to methionyl-tRNAfMet | Escherichia coli JM109 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli JM109 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specificity is restricted to methionyl-tRNAfMet | Escherichia coli CA274 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli CA274 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
additional information | mutant tRNAs as poor substrates | Escherichia coli | ? | - |
? | |
additional information | enzyme structure | Escherichia coli | ? | - |
? | |
additional information | mutant tRNAs as poor substrates | Escherichia coli JM109 | ? | - |
? | |
additional information | enzyme structure | Escherichia coli JM109 | ? | - |
? | |
additional information | mutant tRNAs as poor substrates | Escherichia coli CA274 | ? | - |
? | |
additional information | enzyme structure | Escherichia coli CA274 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.9 | - |
L-methionyl-tRNAfMet | G178E mutant enzyme | Escherichia coli | |
13.9 | - |
L-methionyl-tRNAfMet | wild-type enzyme | Escherichia coli |