KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Euglena gracilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Euglena gracilis | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | partial requirement, in presence of 8 mM Mg2+ optimum concentration is 20 mM | Euglena gracilis | |
Mg2+ | partial requirement, optimum concentration is 8 mM, 3fold increased activity | Euglena gracilis | |
Na+ | activates, can partially replace Mg2+ | Euglena gracilis | |
NH4+ | activates, can partially replace Mg2+ | Euglena gracilis | |
spermine | activates, can partially replace Mg2+ | Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
gel filtration | Euglena gracilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Euglena gracilis | involved in initiation of protein synthesis in chloroplasts, other mechanism than in cytoplasm of eukaryotes | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Euglena gracilis Z | involved in initiation of protein synthesis in chloroplasts, other mechanism than in cytoplasm of eukaryotes | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
- |
- |
Euglena gracilis Z | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1000fold purification from autotrophic cells, 650fold from chloroplasts | Euglena gracilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Euglena gracilis |
0.013 | - |
- |
Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from Euglena gracilis or yeast | Euglena gracilis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from E. coli | Euglena gracilis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | high specificity for initiator tRNAMet, strong positive cooperativity for Met-tRNAfMet and N10-formyltetrahydrofolate | Euglena gracilis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein synthesis in chloroplasts, other mechanism than in cytoplasm of eukaryotes | Euglena gracilis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from Euglena gracilis or yeast | Euglena gracilis Z | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from E. coli | Euglena gracilis Z | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | high specificity for initiator tRNAMet, strong positive cooperativity for Met-tRNAfMet and N10-formyltetrahydrofolate | Euglena gracilis Z | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein synthesis in chloroplasts, other mechanism than in cytoplasm of eukaryotes | Euglena gracilis Z | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Euglena gracilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | - |
Euglena gracilis | |
tetrahydrofolate | - |
Euglena gracilis |