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Literature summary for 2.1.2.9 extracted from

  • Crosti, P.; Gambini, A.; Lucchini, G.; Bianchetti, R.
    Eukaryotic N10-formyl-H4folate:methionyl-tRNAf transformylase. Some properties of the Euglena gracilis enzyme (1977), Biochim. Biophys. Acta, 477, 356-370.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Euglena gracilis 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ partial requirement, in presence of 8 mM Mg2+ optimum concentration is 20 mM Euglena gracilis
Mg2+ partial requirement, optimum concentration is 8 mM, 3fold increased activity Euglena gracilis
Na+ activates, can partially replace Mg2+ Euglena gracilis
NH4+ activates, can partially replace Mg2+ Euglena gracilis
spermine activates, can partially replace Mg2+ Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Euglena gracilis involved in initiation of protein synthesis in chloroplasts, other mechanism than in cytoplasm of eukaryotes tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Euglena gracilis Z involved in initiation of protein synthesis in chloroplasts, other mechanism than in cytoplasm of eukaryotes tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-
Euglena gracilis Z
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1000fold purification from autotrophic cells, 650fold from chloroplasts Euglena gracilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Euglena gracilis
0.013
-
-
Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from Euglena gracilis or yeast Euglena gracilis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Euglena gracilis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O high specificity for initiator tRNAMet, strong positive cooperativity for Met-tRNAfMet and N10-formyltetrahydrofolate Euglena gracilis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein synthesis in chloroplasts, other mechanism than in cytoplasm of eukaryotes Euglena gracilis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from Euglena gracilis or yeast Euglena gracilis Z tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Euglena gracilis Z tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O high specificity for initiator tRNAMet, strong positive cooperativity for Met-tRNAfMet and N10-formyltetrahydrofolate Euglena gracilis Z tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein synthesis in chloroplasts, other mechanism than in cytoplasm of eukaryotes Euglena gracilis Z tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Euglena gracilis

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate
-
Euglena gracilis
tetrahydrofolate
-
Euglena gracilis