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6.3.5.4: asparagine synthase (glutamine-hydrolysing)

This is an abbreviated version!
For detailed information about asparagine synthase (glutamine-hydrolysing), go to the full flat file.

Word Map on EC 6.3.5.4

Reaction

ATP
+
L-aspartate
 +
L-glutamine
 +
H2O
=
AMP
 +
diphosphate
 +
L-asparagine
 +
L-glutamate

Synonyms

AS, AS-A, AS-B, AS1, ASN3, AsnA, ASNase, AsnB, ASNS, AsnS1, AsnS2, AsnS3, AsnS4, asparagine amidotransferase, Asparagine synthetase, Asparagine synthetase (glutamine hydrolyzing), Asparagine synthetase (glutamine), Asparagine synthetase (glutamine-hydrolysing), asparagine synthetase 1, asparagine synthetase 2, Asparagine synthetase A, Asparagine synthetase B, asparagine synthetase, glutamine-dependent, asparagine synthetase1, asparagine synthetase2, At5g10240, bacterial type asparagine synthetase A, CaAS1, glutamine-dependent amidotransferase, Glutamine-dependent asparagine synthetase, HvAS1 protein, HvAS2 protein, HvASN1, HvASN2, HvASN3, HvASN4, HvASN5, L-asparaginase, L-Asparagine synthetase, LDBPK_300470, LiAS-A, MLOC_37219, MLOC_44080, MLOC_63089, MLOC_72774, MLOC_75057, More, NAS2, Os03g0291500, Os06g0265000, OsAS1, OsAS2, PVAS1 protein, PVAS2, Ste10, Synthetase, Asn (glutamine), TaASN1, TaASN2, TaASN4, TaSN1, TaSN2, TS11 cell cycle control protein, type -II asparagine synthetase, type I asparagine synthetase, type II asparagine synthetase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor
                6.3.5.4 asparagine synthase (glutamine-hydrolysing)

Purification

Purification on EC 6.3.5.4 - asparagine synthase (glutamine-hydrolysing)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
development of a method to refold recombinant AsnS from inclusion bodies
immunoaffinity purification
-
one-step immunoaffinity purification
-
partial
recombinant
-
recombinant fusion protein consisting of the 42 kDa N-terminal region of AS and a 17 kDa tagged-region from pET32a(+) expression plasmid
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography to over 95% purity
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ion exchange chromatography
recombinant protein
refolded, solubilized recombinant His-tagged isozyme AsnS1 by nickel affinity chromatography
refolded, solubilized recombinant His-tagged isozyme AsnS2 by nickel affinity chromatography, recombinant nontagged isozyme ZmAsnS2 from Escherichia coli by anion exchange chromatography
refolded, solubilized recombinant His-tagged isozyme AsnS4 by nickel affinity chromatography
refolded, solubilized recombinant His-tagged isozyme by nickel affinity chromatography
using Ni-NTA chromatography
-