6.3.5.4: asparagine synthase (glutamine-hydrolysing)

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For detailed information about asparagine synthase (glutamine-hydrolysing), go to the full flat file.

Reaction

Synonyms

AS, AS-A, AS-B, AS1, ASN3, AsnA, ASNase, AsnB, ASNS, AsnS1, AsnS2, AsnS3, AsnS4, asparagine amidotransferase, Asparagine synthetase, Asparagine synthetase (glutamine hydrolyzing), Asparagine synthetase (glutamine), Asparagine synthetase (glutamine-hydrolysing), asparagine synthetase 1, asparagine synthetase 2, Asparagine synthetase A, Asparagine synthetase B, asparagine synthetase, glutamine-dependent, asparagine synthetase1, asparagine synthetase2, At5g10240, bacterial type asparagine synthetase A, CaAS1, glutamine-dependent amidotransferase, Glutamine-dependent asparagine synthetase, HvAS1 protein, HvAS2 protein, HvASN1, HvASN2, HvASN3, HvASN4, HvASN5, L-asparaginase, L-Asparagine synthetase, LDBPK_300470, LiAS-A, MLOC_37219, MLOC_44080, MLOC_63089, MLOC_72774, MLOC_75057, More, NAS2, Os03g0291500, Os06g0265000, OsAS1, OsAS2, PVAS1 protein, PVAS2, Ste10, Synthetase, Asn (glutamine), TaASN1, TaASN2, TaASN4, TaSN1, TaSN2, TS11 cell cycle control protein, type -II asparagine synthetase, type I asparagine synthetase, type II asparagine synthetase

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor

                6.3.5.4 asparagine synthase (glutamine-hydrolysing)

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Results	in table
8	Activating Compound
20517	AA Sequence
12	Application
1	CAS Registry Number
60	Cloned(Commentary)
14	Cofactor
3	Crystallization (Commentary)
5649	Disease/ Diagnostics
7	Expression
46	General Information
7	General Stability
16	IC50 Value
176	Inhibitors
28	kcat/KM [mM/s]
28	Ki Value [mM]
165	KM Value [mM]
18	Localization
45	Metals/Ions
31	Molecular Weight [Da]
71	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
260	Organism
6	Pathway
6	PDB ID
23	pH Optimum
4	pH Range
1	pH Stability
6	pI Value
2	Posttranslational Modification
55	Protein Variants
34	Purification (Commentary)
12	Reaction
1	Reaction Type
148	Reference
3	Renatured (Commentary)
249	Source Tissue
11	Specific Activity [micromol/min/mg]
5	Storage Stability
206	Substrates and Products (Substrate)
32	Subunits
157	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
6	Temperature Stability [°C]
87	Turnover Number [1/s]

Crystallization

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Crystallization on EC 6.3.5.4 - asparagine synthase (glutamine-hydrolysing)

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

AS-B is dialyzed against 20 mM Bis-Tris, pH 6.5, 150 mM NaCl, 0.5 mM EDTA, and 2 mM dithiothreitol, crystals are grown by hanging drop vapor diffusion at 4°C, AS-B solution is adjusted to 6.5 mg/ml and contains 10 mM MgCl2, 5 mM glutamine, and 10 mM AMP, 0.01 ml of protein solution are mixed with 0.01 ml precipitant solution containing 14% polyethylene glycol 8000 and 50 mM HEPES, pH 7.0, these droplets are suspended against wells containing 15% polyethylene glycol 8000 and 50 mM HEPES, pH 7.0, crystals diffract to 2.0 A

Escherichia coli

P22106

649882

crystal structure analysis, PDB ID 1CT9

Escherichia coli

P22106

745375

X-ray crystal structure of AS-B complexed with glutamine and AMP

Escherichia coli

-

649482