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Literature summary for 6.3.5.4 extracted from

  • Boehlein, S.K.; Nakatsu, T.; Hiratake, J.; Thirumoorty, R.; Stewart, J.D.; Richards, N.G.J.; Schuster, S.M.
    Characterization of inhibitors acting at the synthetase site of Escherichia coli asparagine synthetase B (2001), Biochemistry, 40, 11168-11175.
    View publication on PubMed
Search for more literature for 6.3.5.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia col Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
adenylated sulfoximine 0.005 mM, 65% inhibition, dead-end complex with AS-B Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + L-glutamine Escherichia coli
-
 AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
ATP + L-aspartate + NH3 Escherichia coli asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP AMP + diphosphate + L-asparagine
-
 ?
L-glutamine Escherichia coli
-
 L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + cysteine sulfinic acid
-
 Escherichia coli AMP + diphosphate + cysteine sulfinic acid
-
 ?
ATP + L-aspartate + L-glutamine
-
 Escherichia coli AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
ATP + L-aspartate + NH3 asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP Escherichia coli AMP + diphosphate + L-asparagine
-
 ?
L-glutamine
-
 Escherichia coli L-glutamate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
AS-B
-
 Escherichia coli
Asparagine synthetase B
-
 Escherichia coli