Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP-PNP | competitive vs. ATP, noncompetitive vs. aspartate, uncompetitive vs. glutamine | Escherichia coli | |
asparagine | noncompetitive vs. ATP and aspartate | Escherichia coli | |
beta-methylaspartate | noncompetitive vs. ATP, competitive vs. aspartate, noncompetitive vs. glutamine | Escherichia coli | |
diphosphate | noncompetitive vs. ATP | Escherichia coli | |
L-glutamic acid gamma-methylester | uncompetitive vs. ATP, competitive vs. glutamine | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine | Escherichia coli | - |
AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
ATP + L-aspartate + NH3 | Escherichia coli | asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP | AMP + diphosphate + L-asparagine | - |
? | |
L-glutamine | Escherichia coli | - |
L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine | - |
Escherichia coli | AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
ATP + L-aspartate + NH3 | asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP | Escherichia coli | AMP + diphosphate + L-asparagine | - |
? | |
L-glutamine | - |
Escherichia coli | L-glutamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AS-B | - |
Escherichia coli |
Asparagine synthetase B | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.91 | - |
AMP-PNP | - |
Escherichia coli | |
6.6 | - |
L-glutamic acid gamma-methylester | - |
Escherichia coli | |
18 | - |
beta-methylaspartate | - |
Escherichia coli |