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6.3.5.4: asparagine synthase (glutamine-hydrolysing)

This is an abbreviated version!
For detailed information about asparagine synthase (glutamine-hydrolysing), go to the full flat file.

Word Map on EC 6.3.5.4

Reaction

ATP
+
L-aspartate
+
L-glutamine
+
H2O
=
AMP
+
diphosphate
+
L-asparagine
+
L-glutamate

Synonyms

AS, AS-A, AS-B, AS1, ASN3, AsnA, ASNase, AsnB, ASNS, AsnS1, AsnS2, AsnS3, AsnS4, asparagine amidotransferase, Asparagine synthetase, Asparagine synthetase (glutamine hydrolyzing), Asparagine synthetase (glutamine), Asparagine synthetase (glutamine-hydrolysing), asparagine synthetase 1, asparagine synthetase 2, Asparagine synthetase A, Asparagine synthetase B, asparagine synthetase, glutamine-dependent, asparagine synthetase1, asparagine synthetase2, At5g10240, bacterial type asparagine synthetase A, CaAS1, glutamine-dependent amidotransferase, Glutamine-dependent asparagine synthetase, HvAS1 protein, HvAS2 protein, HvASN1, HvASN2, HvASN3, HvASN4, HvASN5, L-asparaginase, L-Asparagine synthetase, LDBPK_300470, LiAS-A, MLOC_37219, MLOC_44080, MLOC_63089, MLOC_72774, MLOC_75057, More, NAS2, Os03g0291500, Os06g0265000, OsAS1, OsAS2, PVAS1 protein, PVAS2, Ste10, Synthetase, Asn (glutamine), TaASN1, TaASN2, TaASN4, TaSN1, TaSN2, TS11 cell cycle control protein, type -II asparagine synthetase, type I asparagine synthetase, type II asparagine synthetase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor
                6.3.5.4 asparagine synthase (glutamine-hydrolysing)

Molecular Weight

Molecular Weight on EC 6.3.5.4 - asparagine synthase (glutamine-hydrolysing)

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
105000
-
gel filtration
110000
-
gel filtration
110000 - 120000
-
HPLC gel filtration
160000
-
substrate-free enzyme, gel filtration
230000
-
gel filtration
320000
-
in presence of MgCl2 and ATP association to a dimer, gel filtration
52500
57000
-
4 * 57000, denaturing PAGE
60000
-
x * 60000, SDS-PAGE
61000
-
2 * 61000, SDS-PAGE after treatment with dimethyl suberimidate
62000
-
probably 2 * 62000, SDS-PAGE
62666
-
x * 62666, deduced from nucleotide sequence of the asnB gene
62800
x * 62800, about, sequence calculation
64000
SDS-PAGE
64678
x * 64678, calculated
65000
x * 65500, amino acid sequence calculation, x * 65000, Western blot analysis, HvAS1
65182
-
x * 65182, SDS1, deduced from nucleotide sequence
65200
x * 65200, HvAS2, amino acid sequence calculation
65230
-
x * 65230, sequence calculation
65265
x * 65265, sequence calculation
65500
65608
-
x * 65608, SDS2, deduced from nucleotide sequence
65810
-
x * 65810, deduced from nucleotide sequence
66000
-
x * 66000, SDS-PAGE
66500
x * 66500, about, sequence calculation
66800
x * 66800, about, sequence calculation
70000
78800
recombinant enzyme, gel filtration