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6.3.4.5: argininosuccinate synthase

This is an abbreviated version!
For detailed information about argininosuccinate synthase, go to the full flat file.

Word Map on EC 6.3.4.5

Reaction

ATP
+
L-citrulline
 +
L-aspartate
=
AMP
 +
diphosphate
 +
2-(Nomega-L-arginino)succinate

Synonyms

ARGG, Arginine succinate synthetase, argininosuccinate synthase, argininosuccinate synthase 1, Argininosuccinate synthetase, Argininosuccinic acid synthetase, arininosuccinate synthetase, AS, ASS, ASS1, Citrulline--aspartate ligase, Citrulline-aspartate ligase, EAS, Elastin-binding protein, PyARG1, Synthetase, argininosuccinate, tAsS

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.5 argininosuccinate synthase

Crystallization

Crystallization on EC 6.3.4.5 - argininosuccinate synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal strucures in complex with intact ATP and with ATP and citrulline, hanging drop vapour-diffusion technique, X-ray analysis
-
recombinant EAS, hanging drop vapour-diffusion technique, X-ray analysis
-
purified recombinant ASS1 by hanging-drop method, 0.001 ml of protein solution containing 17 mg/ml protein in 500 mM NaCl, 2 mM TCEP, 30 mM HEPES, pH 7.5, 10 mM aspartate, and 10 mM citrulline, is mixed with 0.001 ml of reservoir solution containing 16% w/v PEG 3350, 0.15 M dl-malic acid pH 7.0, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution
3-dimensional structure of free enzyme and of complexes with intact ATP, adenylyl imidodiphosphate, arginine or succinate, hanging drop method
-
structures of enzyme complexed with intact ATP and substrates citrulline and aspartate, complexed with AMP and argininosuccinate, and complexed with AMP-PNP, arginine, aspartate and Mg2+, vapor diffusion method, X-ray analysis