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Literature summary for 6.3.4.5 extracted from

  • Rochovansky, O.; Kodowaki, H.; Ratner, S.
    Biosynthesis of urea. Molecular and regulatory properties of crystalline argininosuccinate synthetase (1977), J. Biol. Chem., 252, 5287-5294.
    View publication on PubMed
Search for more literature for 6.3.4.5

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Bos taurus
AMP
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 citrulline
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46500
-
 4 * 46500, SDS-PAGE in presence of 2-mercaptoethanol Bos taurus
185000
-
 gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.83
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-70°C, stable for 3 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + citrulline + aspartate
-
 Bos taurus AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?
ATP + L-citrulline + L-Asp
-
 Bos taurus AMP + diphosphate + L-argininosuccinate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 46500, SDS-PAGE in presence of 2-mercaptoethanol Bos taurus