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6.3.4.5: argininosuccinate synthase

This is an abbreviated version!
For detailed information about argininosuccinate synthase, go to the full flat file.

Word Map on EC 6.3.4.5

Reaction

ATP
+
L-citrulline
 +
L-aspartate
=
AMP
 +
diphosphate
 +
2-(Nomega-L-arginino)succinate

Synonyms

ARGG, Arginine succinate synthetase, argininosuccinate synthase, argininosuccinate synthase 1, Argininosuccinate synthetase, Argininosuccinic acid synthetase, arininosuccinate synthetase, AS, ASS, ASS1, Citrulline--aspartate ligase, Citrulline-aspartate ligase, EAS, Elastin-binding protein, PyARG1, Synthetase, argininosuccinate, tAsS

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.5 argininosuccinate synthase

Specific Activity

Specific Activity on EC 6.3.4.5 - argininosuccinate synthase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000001
-
37°C, cell homogenate
0.000006
-
37°C, A-549 cell homogenate
0.000013
-
37°C, LNCaP cell homogenate
0.000019
-
37°C, MCF-7 cell homogenate
0.000048
-
37°C, HeLa cell homogenate
0.000174
-
37°C, cell homogenate
0.0002
-
pH 8.7, 70°C, activity in cell culture in growth medium 0.5% peptone and 0.1% yeast extract
0.00045
-
pH 8.7, 70°C, activity in cell culture in growth medium with 0.2% yeast extract
0.0007
-
pH 8.7, 70°C, activity in cell culture in minimal growth medium with 20 mM glucose and 5 mM NH4+
0.002
-
in the presence of 5 mM (NH4)2SO4 and after addition of 20 mM L-norvaline in root-free compartments
0.0028
-
in the presence of 5 mM (NH4)2SO4 and after addition of 20 mM L-norvaline in the root-free compartments
0.0071
-
in the presence of 5 mM (NH4)2SO4 and after addition of 0.5 mM phenyl phosphorodiamidate in root-free compartments
0.0085
-
in the presence of 5 mM (NH4)2SO4 in root-free compartments
0.0166
0.049
0.14
-
purification step: liver homogenate supernatant
0.27
-
purification step: 45-55% saturated ammonium sulfate precipitate
0.95
-
-
0.99
-
purification step: DE-52 fraction
1
-
specific activity of ASS in Caco-2 cells after long time exposure (24h) to 1 ng/ml IL-1beta
1.27
-
specific activity of ASS in Caco-2 cells after the addition of 0.5 mM S-nitroso-acetylpenicillamine for 24h, an NO donor. This clearly demonstrates that NO induces an inhibition of the ASS activity in Caco-2 cells.
1.3
-
-
1.39
-
purification step: S-300 fraction
12.8
-
-
2.5
-
specific activity of ASS in Caco-2 cells after short time exposure (4h) to 1 ng/ml IL-1beta
3.8
-
-
3.83
-
-
4.2
-
-
5.16
-
purification step: UNO S-1 fraction