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6.3.4.5: argininosuccinate synthase

This is an abbreviated version!
For detailed information about argininosuccinate synthase, go to the full flat file.

Word Map on EC 6.3.4.5

Reaction

ATP
+
L-citrulline
 +
L-aspartate
=
AMP
 +
diphosphate
 +
2-(Nomega-L-arginino)succinate

Synonyms

ARGG, Arginine succinate synthetase, argininosuccinate synthase, argininosuccinate synthase 1, Argininosuccinate synthetase, Argininosuccinic acid synthetase, arininosuccinate synthetase, AS, ASS, ASS1, Citrulline--aspartate ligase, Citrulline-aspartate ligase, EAS, Elastin-binding protein, PyARG1, Synthetase, argininosuccinate, tAsS

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.5 argininosuccinate synthase

Cloned

Cloned on EC 6.3.4.5 - argininosuccinate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in Escherichia coli
expressed as a C-terminal fusion protein with maltose-binding protein in Escherichia coli
-
expressed in Escherichia coli BL21 (DE3) pLysS cells
expression alone or together with FLAG-tagged HSCARG or with HSCARG siRNA in HeLa and HEK-293 cells, expression analysis, overview
expression analysis, the AS gene promoter contains a a near consensus PPARgamma response element, PPRE, overview
-
expression in Escherichia coli
expression in Escherichia coli as fusion protein with maltose binding protein
-
expression in Escherichia coli BB101
-
expression in Escherichia coli BL21(DE3)pLysE
expression of ASS1
expression of the His-tagged enzyme in Escherichia coli
-
overexpression in Escherichia coli
-
the gene encoding argininosuccinate synthetase of Porphyra yezoensis is obtained by PCR using an expressed sequence tag clone as a template, and subcloned into the yeast expression vector pYES2. The gene is expressed when the vector harboring PyARG1 is introduced into an ARG1-deficient strain of Saccharomyces cerevisiae, which results in complementation of the mutant phenotype. The transformed cells survive on a selective medium lacking arginine, and transcripts of PyARG1 are detected by RT-PCR. A quantitative comparison shows that the rescued mutant cells grow in the selective liquid medium with a minor reduction in growth rate relative to wild-type cells