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6.3.4.2: CTP synthase (glutamine hydrolysing)

This is an abbreviated version!
For detailed information about CTP synthase (glutamine hydrolysing), go to the full flat file.

Word Map on EC 6.3.4.2

Reaction

ATP
+
UTP
+
L-glutamine
=
ADP
+
phosphate
+
CTP
+
L-glutamate

Synonyms

CTP synthase, CTP synthase 1, CTP synthetase, CTP synthetase 1, CTPS, CTPS1, CTPS2, CTPsyn, CTS, cytidine 5'-triphosphate synthase, cytidine 5'-triphosphate synthetase, cytidine triphosphate synthetase, cytidine triphosphate synthetase 1, EcCTPS, pfCTP synthetase, PyrG, synthetase, cytidine triphosphate, URA7, uridine triphosphate aminase, UTP-ammonia ligase, UTP:ammonia ligase (ADP-forming)

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.2 CTP synthase (glutamine hydrolysing)

Storage Stability

Storage Stability on EC 6.3.4.2 - CTP synthase (glutamine hydrolysing)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 30% glycerol, 100 mM 2-mercaptoethanol, 40% loss of activity after 4 weeks
-
-20°C, purified enzyme is stable for months
-
-20°C, stable for several months
-
-80°C, 30% glycerol, 100 mM 2-mercaptoethanol, 65% of the original activity is retained after 4 weeks
-
-80°C, stable for at least 6 months
-
4°C, 30% glycerol, 100 mM 2-mercaptoethanol, 65% loss of activity after 4 weeks
-
4°C, enzyme concentration 1-4 mg/ml, 20 mM sodium phosphate buffer, pH 7.2, 2 mM L-glutamine, 1 mM EDTA, 70 mM 2-mercaptoethanol, 20% glycerol, stable for 3 months
-