6.3.4.2: CTP synthase (glutamine hydrolysing)
This is an abbreviated version!
For detailed information about CTP synthase (glutamine hydrolysing), go to the full flat file.
Word Map on EC 6.3.4.2
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6.3.4.2
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pyrimidine
-
cytosine
-
cytoophidia
-
dctp
-
cyclopentenyl
-
deoxycytidine
-
acivicin
-
glutamine-dependent
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3-deazauridine
-
rheumatology
-
l-glutamine
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alliance
-
carra
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ctpas
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pyrazofurin
-
uridine-cytidine
-
rheumatologist
-
6-diazo-5-oxo-l-norleucine
-
drug development
-
medicine
-
analysis
- 6.3.4.2
- pyrimidine
- cytosine
-
cytoophidia
- dctp
-
cyclopentenyl
- deoxycytidine
- acivicin
-
glutamine-dependent
- 3-deazauridine
-
rheumatology
- l-glutamine
-
alliance
-
carra
-
ctpas
- pyrazofurin
-
uridine-cytidine
-
rheumatologist
- 6-diazo-5-oxo-l-norleucine
- drug development
- medicine
- analysis
Reaction
Synonyms
CTP synthase, CTP synthase 1, CTP synthetase, CTP synthetase 1, CTPS, CTPS1, CTPS2, CTPsyn, CTS, cytidine 5'-triphosphate synthase, cytidine 5'-triphosphate synthetase, cytidine triphosphate synthetase, cytidine triphosphate synthetase 1, EcCTPS, pfCTP synthetase, PyrG, synthetase, cytidine triphosphate, URA7, uridine triphosphate aminase, UTP-ammonia ligase, UTP:ammonia ligase (ADP-forming)
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Reference on EC 6.3.4.2 - CTP synthase (glutamine hydrolysing)
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Iyengar, A.; Bearne, S.L.
Aspartate-107 and leucine-109 facilitate efficient coupling of glutamine hydrolysis to CTP synthesis by Escherichia coli CTP synthase
Biochem. J.
369
497-507
2003
Escherichia coli
Bearne, S.L.; Hekmat, O.; Macdonnell, J.E.
Inhibition of Escherichia coli CTP synthase by glutamate gamma-semialdehyde and the role of the allosteric effector GTP in glutamine hydrolysis
Biochem. J.
356
223-232
2001
Escherichia coli
Willemoes, M.; Sigurskjold, B.W.
Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis. The role of the allosteric activator GTP incoupling between glutamine hydrolysis and CTP synthesis
Eur. J. Biochem.
269
4772-4779
2002
Lactococcus lactis
Verschuur, A.C.; van Gennip, A.H.; Muller, E.J.; Voute, P.A.; Vreken, P.; Van Kuilenburg, A.B.
Cytidine triphosphate synthase activity and mRNA expression in normal human blood cells
Biol. Chem.
380
41-46
1999
Homo sapiens
van Kuilenburg, A.B.; Meinsma, R.; Vreken, P.; Waterham, H.R.; van Gennip, A.H.
Identification of a cDNA encoding an isoform of human CTP synthetase
Biochim. Biophys. Acta
1492
548-552
2000
Escherichia coli (P0A7E5), Escherichia coli, Homo sapiens (P17812), Homo sapiens (Q9NRF8), Homo sapiens, Saccharomyces cerevisiae (P28274), Saccharomyces cerevisiae (P38627), Mus musculus (P70303), Mus musculus (P70698)
Verschuur, A.C.; Van Gennip, A.H.; Leen, R.; Meinsma, R.; Voute, P.A.; van Kuilenburg, A.B.
In vitro inhibition of cytidine triphosphate synthetase activity by cyclopentenyl cytosine in paediatric acute lymphocytic leukaemia
Br. J. Haematol.
110
161-169
2000
Homo sapiens
Willemoes, M.; Larsen, S.
Substrate inhibition of Lactococcus lactis cytidine 5'-triphosphate synthase by ammonium chloride is enhanced by salt-dependent tetramer dissociation
Arch. Biochem. Biophys.
413
17-22
2003
Lactococcus lactis
van Kuilenburg, A.B.P.; Elzinga, L.; Van Gennip, A.H.
Kinetic properties of CTP synthetase from HL-60 cells
Adv. Exp. Med. Biol.
431
255-258
1998
Homo sapiens
van Kuilenburg, A.B.P.; Meinsma, R.; Vreken, P.; Waterham, H.R.; van Gennip, A.H.
Isoforms of human CTP synthetase
Adv. Exp. Med. Biol.
486
257-261
2000
Homo sapiens
Meng, Q.; Switzer, R.L.
Regulation of transcription of the Bacillus subtilis pyrG gene, encoding cytidine triphosphate synthetase
J. Bacteriol.
183
5513-5522
2001
Bacillus subtilis
Hofer, A.; Steverding, D.; Chabes, A.; Brun, R.; Thelander, L.
Trypanosoma brucei CTP synthetase: a target for the treatment of African sleeping sickness
Proc. Natl. Acad. Sci. USA
98
6412-6416
2001
Trypanosoma brucei
Park, T.S.; O'Brien, D.J.; Carman, G.M.
Phosphorylation of CTP synthetase on Ser36, Ser330, Ser354, and Ser454 regulates the levels of CTP and phosphatidylcholine synthesis in Saccharomyces cerevisiae
J. Biol. Chem.
278
20785-20794
2003
Saccharomyces cerevisiae
Jorgensen, C.M.; Hammer, K.; Martinussen, J.
CTP limitation increases expression of CTP synthase in Lactococcus lactis
J. Bacteriol.
185
6562-6574
2003
Lactococcus lactis
Pappas, A.; Yang, W.L.; Park, T.S.; Carman, G.M.
Nucleotide-dependent tetramerization of CTP synthetase from Saccharomyces cerevisiae
J. Biol. Chem.
273
15954-15960
1998
Saccharomyces cerevisiae
Ostrander, D.B.; O'Brien, D.J.; Gorman, J.A.; Carman, G.M.
Effect of CTP synthetase regulation by CTP on phospholipid synthesis in Saccharomyces cerevisiae
J. Biol. Chem.
273
18992-19001
1998
Saccharomyces cerevisiae
Wadskov-Hansen, S.L.; Willemoes, M.; Martinussen, J.; Hammer, K.; Neuhard, J.; Larsen, S.
Cloning and verification of the Lactococcus lactis pyrG gene and characterization of the gene product, CTP synthase
J. Biol. Chem.
276
38002-38009
2001
Lactococcus lactis
Pappas, A.; Park, T.S.; Carman, G.M.
Characterization of a novel dUTP-dependent activity of CTP synthetase from Saccharomyces cerevisiae
Biochemistry
38
16671-16677
1999
Saccharomyces cerevisiae
Goto, M.; Omi, R.; Hoseki, J.; Nakagawa, N.; Miyahara, I.; Hirotsu, K.
Expression, purification and preliminary X-ray characterization of CTP synthetase from Thermus thermophilus HB8
Acta Crystallogr. Sect. D
59
551-553
2003
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Weng, M.; Zalkin, H.
Structural role for a conserved region in the CTP synthetase glutamine amide transfer domain
J. Bacteriol.
169
3023-3028
1987
Escherichia coli
Zalkin, H.
CTP synthetase
Methods Enzymol.
113
282-287
1985
Escherichia coli, Escherichia coli B / ATCC 11303
Kizaki, H.; Ohsaka, F.; Sakurada, T.
CTP synthetase from Ehrlich ascites tumor cells. Subunit stoichiometry and regulation of activity
Biochim. Biophys. Acta
829
34-43
1985
Mus musculus
von der Saal, W.; Villafranca, J.J.; Anderson, P.M.
Cytidine-5'-triphosphate synthetase catalyzes the phosphorylation of uridine 5'-triphosphate by adenosine 5'-triphosphate
J. Am. Chem. Soc.
107
703-704
1985
Chlamydia trachomatis, Escherichia coli
-
Anderson, P.M.
CTP synthetase from Escherichia coli: an improved purification procedure and characterization of hysteretic and enzyme concentration effects on kinetic properties
Biochemistry
22
3285-3292
1983
Escherichia coli
Kizaki, H.; Ohsaka, F.; Sakurada, T.
Role of GTP in CTP synthetase from Ehrlich ascites tumor cells
Biochem. Biophys. Res. Commun.
108
286-291
1982
Mus musculus
Scheit, K.H.; Linke, H.J.
Substrate specificity of CTP synthetase from Escherichia coli
Eur. J. Biochem.
126
57-60
1982
Escherichia coli
Kizaki, H.; Sakurada, T.; Weber, G.
Purification and properties of CTP synthetase from Ehrlich ascites tumor cells
Biochim. Biophys. Acta
662
48-54
1981
Mus musculus
Weinfeld, H.; Savage, C.R.; McPartland, R.P.
CTP synthetase of bovine calf liver
Methods Enzymol.
51
84-90
1978
Bos taurus
Long, C.; Koshland jr., D.E.
Cytidine triphosphate synthetase
Methods Enzymol.
51
79-83
1978
Escherichia coli
Williams, J.C.; Kizaki, H.; Weber, G.; Morris, H.P.
Increased CTP synthetase activity in cancer cells
Nature
271
71-73
1978
Rattus norvegicus
McPartland, R.P.; Weinfeld, H.
Cytidine 5'-triphosphate synthetase of calf liver
J. Biol. Chem.
251
4372-4378
1976
Bos taurus
Koshland jr., D.E.; Levitzki, A.
CTP synthetase and related enzymes
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
10
539-559
1974
Escherichia coli
-
Scheit, K.H.; Linke, H.J.
Substrate specificity of CTP synthetase from E. coli
Nucleic Acids Res.
9
229-233
1981
Escherichia coli
Yang, W.L.; Carman, G.M.
Phosphorylation and regulation of CTP synthetase from Saccharomyces cerevisiae by protein kinase A
J. Biol. Chem.
271
28777-28783
1996
Saccharomyces cerevisiae
Wylie, J.L.; Wang, L.L.; Tipples, G.; McClarty, G.
A single point mutation in CTP synthetase of Chlamydia trachomatis confers resistance to cyclopentenyl cytosine
J. Biol. Chem.
271
15393-15400
1996
Chlamydia trachomatis, Chlamydia trachomatis L2/CPEC
Kuilenburg, A.B.P.; Elzinga, L.; van den Berg, A.A.; Slingerland, R.J.; van Gennip, A.H.
A fast and novel assay of CTP synthetase. Evidence for hysteretic properties of the mammalian enzyme
Anticancer Res.
14
411-416
1994
Bos taurus
Lewis, D.A.; Villafranca, J.J.
Investigation of the mechanism of CTP synthetase using rapid quench and isotope partitioning methods
Biochemistry
28
8454-8459
1989
Escherichia coli
Kang, G.J.; Cooney, D.A.; Moyer, J.D.; Kelley, J.A.; Kim, H.Y.; Marquez, V.E.; Johns, D.G.
Cyclopentenylcytosine triphosphate. Formation and inhibition of CTP synthetase
J. Biol. Chem.
264
713-718
1989
Mus musculus
Yang, W.L.; McDonough, V.M.; Ozier-Kalogeropoulos, O.; Adeline, M.T.; Flocco, M.T.; Carman, G.M.
Purification and characterization of CTP synthetase, the product of the URA7 gene in Saccharomyces cerevisiae
Biochemistry
33
10785-10793
1994
Saccharomyces cerevisiae
Wylie, J.L.; Berry, J.D.; McClarty, G.
Chlamydia trachomatis CTP synthetase: molecular characterization and developmental regulation of expression
Mol. Microbiol.
22
631-642
1996
Chlamydia trachomatis, Escherichia coli
Aronow, B.; Ullman, B.
In situ regulation of mammalian CTP synthetase by allosteric inhibition
J. Biol. Chem.
262
5106-5112
1987
Mus musculus
Robertson, J.G.; Villafranca, J.J.
Characterization of metal ion activation and inhibition of CTP synthetase
Biochemistry
32
3769-3777
1993
Escherichia coli
Kizaki, H.; Ohsaka, F.; Sakurada, T.
Synthesis of N4-substituted CTP by mammalian CTP synthetase
Biochem. Biophys. Res. Commun.
145
569-574
1987
Mus musculus
Willemös, M.
Competition between ammonia derived from internal glutamine hydrolysis and hydroxylamine present in the solution for incorporation into UTP as catalysed by Lactococcus lactis CTP synthase
Arch. Biochem. Biophys.
424
105-111
2004
Lactococcus lactis
Endrizzi, J.A.; Kim, H.; Anderson, P.M.; Baldwin, E.P.
Crystal structure of Escherichia coli cytidine triphosphate synthetase, a nucleotide-regulated glutamine amidotransferase/ATP-dependent amidoligase fusion protein and homologue of anticancer and antiparasitic drug targets
Biochemistry
43
6447-6463
2004
Escherichia coli (P0A7E5), Escherichia coli
MacDonnell, J.E.; Lunn, F.A.; Bearne, S.L.
Inhibition of E. coli CTP synthase by the "positive" allosteric effector GTP
Biochim. Biophys. Acta
1699
213-220
2004
Escherichia coli
Lunn, F.A.; Bearne, S.L.
Alternative substrates for wild-type and L109A E. coli CTP synthases: kinetic evidence for a constricted ammonia tunnel
Eur. J. Biochem.
271
4204-4212
2004
Escherichia coli (P0A7E5), Escherichia coli
Willemös, M.; Molgaard, A.; Johansson, E.; Martinussen, J.
Lid L11 of the glutamine amidotransferase domain of CTP synthase mediates allosteric GTP activation of glutaminase activity
FEBS J.
272
856-864
2005
Lactococcus lactis
Yuan, P.; Hendriks, E.F.; Fernandez, H.R.; O'Sullivan, W.J.; Stewart, T.S.
Functional expression of the gene encoding cytidine triphosphate synthetase from Plasmodium falciparum which contains two novel sequences that are potential antimalarial targets
Mol. Biochem. Parasitol.
143
200-208
2005
Plasmodium falciparum
Goto, M.; Omi, R.; Nakagawa, N.; Miyahara, I.; Hirotsu, K.
Crystal structures of CTP synthetase reveal ATP, UTP, and glutamine binding sites
Structure
12
1413-1423
2004
Thermus thermophilus HB8 (Q5SIA8), Thermus thermophilus HB8
Kursula, P.; Flodin, S.; Ehn, M.; Hammarstroem, M.; Schueler, H.; Nordlund, P.; Stenmark, P.
Structure of the synthetase domain of human CTP synthetase, a target for anticancer therapy
Acta Crystallogr. Sect. F
62
613-617
2006
Homo sapiens
MacLeod, T.J.; Lunn, F.A.; Bearne, S.L.
The role of lysine residues 297 and 306 in nucleoside triphosphate regulation of E. coli CTP synthase: inactivation by 2,3-dialdehyde ATP and mutational analyses
Biochim. Biophys. Acta
1764
199-210
2006
Escherichia coli (A0A140N932), Escherichia coli
Fijolek, A.; Hofer, A.; Thelander, L.
Expression, purification, characterization, and in vivo targeting of trypanosome CTP synthetase for treatment of African sleeping sickness
J. Biol. Chem.
282
11858-11865
2007
Trypanosoma brucei
Chang, Y.F.; Martin, S.S.; Baldwin, E.P.; Carman, G.M.
Phosphorylation of human CTP synthetase 1 by protein kinase C: identification of Ser(462) and Thr(455) as major sites of phosphorylation
J. Biol. Chem.
282
17613-17622
2007
Homo sapiens
Higgins, M.J.; Graves, P.R.; Graves, L.M.
Regulation of human cytidine triphosphate synthetase 1 by glycogen synthase kinase 3
J. Biol. Chem.
282
29493-29503
2007
Homo sapiens (Q9NRF8), Homo sapiens
Choi, M.G.; Carman, G.M.
Phosphorylation of human CTP synthetase 1 by protein kinase A: identification of Thr455 as a major site of phosphorylation
J. Biol. Chem.
282
5367-5377
2007
Homo sapiens
Lunn, F.A.; Macdonnell, J.E.; Bearne, S.L.
Structural requirements for the activation of Escherichia coli CTP synthase by the allosteric effector GTP are stringent, but requirements for inhibition are Lax
J. Biol. Chem.
283
2010-2020
2007
Escherichia coli (P0A7E5), Escherichia coli
Braun, O.; Knipp, M.; Chesnov, S.; Vasak, M.
Specific reactions of S-nitrosothiols with cysteine hydrolases: A comparative study between dimethylargininase-1 and CTP synthetase
Protein Sci.
16
1522-1534
2007
Escherichia coli (P0A7E5)
Lunn, F.A.; Macleod, T.J.; Bearne, S.L.
Mutational analysis of conserved glycine residues 142, 143 and 146 reveals Gly(142) is critical for tetramerization of CTP synthase from Escherichia coli
Biochem. J.
412
113-121
2008
Escherichia coli (P0A7E5), Escherichia coli
Higgins, M.J.; Loiselle, D.; Haystead, T.A.; Graves, L.M.
Human cytidine triphosphate synthetase 1 interacting proteins
Nucleosides Nucleotides Nucleic Acids
27
850-857
2008
Homo sapiens
Chang, Y.F.; Carman, G.M.
CTP synthetase and its role in phospholipid synthesis in the yeast Saccharomyces cerevisiae
Prog. Lipid Res.
47
333-339
2008
Saccharomyces cerevisiae, Homo sapiens
Roy, A.C.; Lunn, F.A.; Bearne, S.L.
Inhibition of CTP synthase from Escherichia coli by xanthines and uric acids
Bioorg. Med. Chem. Lett.
20
141-144
2010
Escherichia coli
Lauritsen, I.; Willemoes, M.; Jensen, K.F.; Johansson, E.; Harris, P.
Structure of the dimeric form of CTP synthase from Sulfolobus solfataricus
Acta Crystallogr. Sect. F
67
201-208
2011
Saccharolobus solfataricus (Q980S6), Saccharolobus solfataricus
Kassel, K.M.; Au, d.a..R.; Higgins, M.J.; Hines, M.; Graves, L.M.
Regulation of human cytidine triphosphate synthetase 2 by phosphorylation
J. Biol. Chem.
285
33727-33736
2010
Homo sapiens, Homo sapiens (Q9NRF8)
Liu, J.L.
Intracellular compartmentation of CTP synthase in Drosophila
J. Genet. Genomics
37
281-296
2010
Drosophila melanogaster
Ingerson-Mahar, M.; Briegel, A.; Werner, J.N.; Jensen, G.J.; Gitai, Z.
The metabolic enzyme CTP synthase forms cytoskeletal filaments
Nat. Cell Biol.
12
739-746
2010
Caulobacter vibrioides, Escherichia coli
Steeves, C.H.; Bearne, S.L.
Activation and inhibition of CTP synthase from Trypanosoma brucei, the causative agent of African sleeping sickness
Bioorg. Med. Chem. Lett.
21
5188-5190
2011
Trypanosoma brucei
Gou, K.M.; Chang, C.C.; Shen, Q.J.; Sung, L.Y.; Liu, J.L.
CTP synthase forms cytoophidia in the cytoplasm and nucleus
Exp. Cell Res.
323
242-253
2014
Homo sapiens, Mus musculus
Azzam, G.; Liu, J.L.
Only one isoform of Drosophila melanogaster CTP synthase forms the cytoophidium
PLoS Genet.
9
e1003256
2013
Drosophila melanogaster
Strochlic, T.I.; Stavrides, K.P.; Thomas, S.V.; Nicolas, E.; OReilly, A.M.; Peterson, J.R.
Ack kinase regulates CTP synthase filaments during Drosophila oogenesis
EMBO Rep.
15
1184-1191
2014
Drosophila melanogaster (X2JCY2)
Huang, Y.; Wang, J.J.; Ghosh, S.; Liu, J.L.
Critical roles of CTP synthase N-terminal in cytoophidium assembly
Exp. Cell Res.
354
122-133
2017
Drosophila melanogaster (X2JCY2)
Chang, C.C.; Jeng, Y.M.; Peng, M.; Keppeke, G.D.; Sung, L.Y.; Liu, J.L.
CTP synthase forms the cytoophidium in human hepatocellular carcinoma
Exp. Cell Res.
361
292-299
2017
Homo sapiens, Drosophila melanogaster (X2JCY2)
Wang, P.Y.; Lin, W.C.; Tsai, Y.C.; Cheng, M.L.; Lin, Y.H.; Tseng, S.H.; Chakraborty, A.; Pai, L.M.
Regulation of CTP synthase filament formation during DNA endoreplication in Drosophila
Genetics
201
1511-1523
2015
Drosophila melanogaster (X2JCY2)
Li, X.; Xie, J.; Hei, M.; Tang, J.; Wang, Y.; Foerster, E.; Zhao, S.
High level of CTP synthase induces formation of cytoophidia in cortical neurons and impairs corticogenesis
Histochem. Cell Biol.
149
61-73
2018
Mus musculus
Noree, C.; Monfort, E.; Shiau, A.K.; Wilhelm, J.E.
Common regulatory control of CTP synthase enzyme activity and filament formation
Mol. Biol. Cell
25
2282-2290
2014
Saccharomyces cerevisiae (P28274), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (P28274)
Aughey, G.; Grice, S.; Liu, J.
The Interplay between Myc and CTP synthase in Drosophila
PLoS Genet.
12
e1005867
2016
Drosophila melanogaster (X2JCY2)
Sandalli, C.; Saral, A.; Uelker, S.; Karaoglu, H.; Belduez, A.; Copur Cicek, A.
Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2
Turk. J. Biol.
38
111-117
2014
Anoxybacillus gonensis (A0A0A2SLZ4), Anoxybacillus gonensis G2 (A0A0A2SLZ4)
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