Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.3.4.2: CTP synthase (glutamine hydrolysing)

This is an abbreviated version!
For detailed information about CTP synthase (glutamine hydrolysing), go to the full flat file.

Word Map on EC 6.3.4.2

Reaction

ATP
+
UTP
 +
L-glutamine
=
ADP
 +
phosphate
 +
CTP
 +
L-glutamate

Synonyms

CTP synthase, CTP synthase 1, CTP synthetase, CTP synthetase 1, CTPS, CTPS1, CTPS2, CTPsyn, CTS, cytidine 5'-triphosphate synthase, cytidine 5'-triphosphate synthetase, cytidine triphosphate synthetase, cytidine triphosphate synthetase 1, EcCTPS, pfCTP synthetase, PyrG, synthetase, cytidine triphosphate, URA7, uridine triphosphate aminase, UTP-ammonia ligase, UTP:ammonia ligase (ADP-forming)

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.2 CTP synthase (glutamine hydrolysing)

Cloned

Cloned on EC 6.3.4.2 - CTP synthase (glutamine hydrolysing)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a carboxyl-terminal FLAG sequence is introduced after insertion of human CTPS1 into pCDNA 4 myc/his, expression by transient transfection of human embryonic kidney (HEK) 293 cells
cDNA encoding the synthetase domain of human CTPS (isoform 1) is subcloned into the pNIC-Bsa4 vector, the resulting construct codes for a protein with an N-terminal hexahistidine tag with an integrated TEV protease-cleavage site, the entire plasmid is transformed in Escherichia coli BL-21
-
cloned in Escherichia coli as a histidine-tagged fusion protein
cloned in Escherichia coli BL-21 as an N-terminal 6xHis-tag fusion protein
expressed in Escherichia coli BL21(DE3) cells
expression in a ura7ura8 double mutant that lacks CTP synthetase activity
-
expression in Escherichia coli
expression of the human type II CTP synthetase gene in the CTP synthetase-deficient mutant Escherichia coli JF618 completely abolishes the need for exogenous cytidine for their survival
-
expression of the mutant enzyme D149E in Escherichia coli
-
expression of wild-type and mutant enzymes in HEK-293 cells, co-expression with GST-tagged Xenopus laevis peptidyl prolyl isomerase Pin1
-
functional expression of human CTPS1 and CTPS2 genes that encode CTP synthetase enzymes in enzyme-deficient Saccharomyces cerevisiae ura7D/ura8D double mutant, complementation
-
genes ura7D and ura8D, DNA and amino acid sequence determination and analysis, expression analysis
-
human CTP synthetase 1 is expressed and purified from a Saccharomyces cerevisiae ura7delta ura8delta double mutant that lacks CTP synthetase activity
-
overexpression as a hexahistidine-tagged form
-
protein is expressed as a His6-tagged wild type human CTP synthetase 1 fusion protein in Escherichia coli
-
protein is expressed as a His6-tagged wild type human CTP synthetase 1 fusion protein in Saccharomyces cerevisiae
-
synthetase-His6-TEV protease site-tagged Trypanosoma brucei CTP synthetase is expressed in BL21(DE3) pLysS bacteria
-
wild-type and L109A recombinant enzyme are expressed in Escherichia coli BL21(DE3)