6.3.2.5: phosphopantothenate-cysteine ligase (CTP)

This is an abbreviated version!
For detailed information about phosphopantothenate-cysteine ligase (CTP), go to the full flat file.

Reaction

Synonyms

4�-phosphopantothenoylcysteine synthetase, CoaB, CoaBC, coupling enzyme, Dfp, pantothenate 4�-phosphate:L-cysteine ligase, phosphopantothenate-cysteine ligase, phosphopantothenoyl-cysteine ligase, phosphopantothenoylcysteine synthetase, PPC synthase, PPC synthetase, PPC-S, PPCS, synthetase, phosphopantothenoylcysteine

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.2 Acid—amino-acid ligases (peptide synthases)

                6.3.2.5 phosphopantothenate-cysteine ligase (CTP)

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Results	in table
35953	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
3	Cofactor
2	Crystallization (Commentary)
11	Disease/ Diagnostics
3	General Information
14	Inhibitors
7	Ki Value [mM]
20	KM Value [mM]
2	Metals/Ions
6	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
27	Organism
6	Pathway
34	PDB ID
2	pH Optimum
18	Protein Variants
8	Purification (Commentary)
6	Reaction
2	Reaction Type
27	Reference
8	Source Tissue
3	Specific Activity [micromol/min/mg]
38	Substrates and Products (Substrate)
7	Subunits
27	Synonyms
1	Systematic Name
5	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 6.3.2.5 - phosphopantothenate-cysteine ligase (CTP)

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

ATP + (R)-4'-phosphopantothenate + 2-aminoethanethiol disulfide

?

2 entries

ATP + (R)-4'-phosphopantothenate + 2-methylcysteine

N-(phosphopantothenyl)-alpha-methylcysteine + ADP + phosphate

2 entries

ATP + (R)-4'-phosphopantothenate + beta-mercaptoethylamine

phosphopantetheine + ADP + phosphate

2 entries

ATP + (R)-4'-phosphopantothenate + cystine

(R)-4'-phosphopantothenoyl-L-cystine + ADP + phosphate

Morganella morganii

-

5% of activity compared to L-Cys

4406

-

-

?

ATP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate

8 entries

CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine

CTP + (R)-4'-phosphopantothenate + L-cysteine

Escherichia coli

-

-

648960

-

-

r

CTP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate

9 entries

CTP + (R)-4'-phosphopantothenate + L-Cys

?

Micrococcus luteus

-

Brown´s pathway of CoA synthesis

4412

-

-

?

CTP + (R)-4'-phosphopantothenate + L-cysteine

CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine

7 entries

additional information

?

-

Escherichia coli

-

ATP, GTP, and UTP cannot substitute for CTP

648960

-

-

?

ATP + (R)-4'-phosphopantothenate + 2-aminoethanethiol disulfide

?

Morganella morganii

-

10% of activity compared to L-Cys

4406

-

-

?

ATP + (R)-4'-phosphopantothenate + 2-aminoethanethiol disulfide

?

Rattus norvegicus

-

-

4406, 648952

-

-

?

ATP + (R)-4'-phosphopantothenate + 2-methylcysteine

N-(phosphopantothenyl)-alpha-methylcysteine + ADP + phosphate

Morganella morganii

-

10% of the activity compared to L-cysteine

4406

-

4406

?

ATP + (R)-4'-phosphopantothenate + 2-methylcysteine

N-(phosphopantothenyl)-alpha-methylcysteine + ADP + phosphate

Rattus norvegicus

-

-

4406, 648952

-

-

?

ATP + (R)-4'-phosphopantothenate + beta-mercaptoethylamine

phosphopantetheine + ADP + phosphate

Morganella morganii

-

25% of activity compared to L-Cys

4406

-

4406

?

ATP + (R)-4'-phosphopantothenate + beta-mercaptoethylamine

phosphopantetheine + ADP + phosphate

Rattus norvegicus

-

-

4406, 648952

-

-

?

ATP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate

Enterococcus faecalis

-

8% of the activity with CTP

702284

-

-

?

ATP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate

Homo sapiens

-

-

648959

-

-

?

ATP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate

Homo sapiens

-

human enzyme can use both ATP and CTP with similar affinity. Catalysis under CTP conditions displays Michaelis-Menten kinetics

702454

-

-

?

ATP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate

Mammalia

-

mammalian enzyme differs from the bacterial enzyme in that it can use any of the other nucleotide triphosphates equally as well as CTP

4406

-

-

?

ATP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate

Rattus norvegicus

-

-

4406

-

-

?

ATP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate

Rattus norvegicus

-

-

648952

-

648952

?

ATP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate

Rattus norvegicus

-

-

648953

-

648953

?

ATP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate

Rattus norvegicus

-

-

648954

-

-

?

CTP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate

Bacteria

-

mammalian enzyme differs from the bacterial enzyme in that it can use any of the other nucleotide triphosphates equally as well as CTP

4406

-

-

?

CTP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate

Corynebacterium ammoniagenes

-

-

648951, 648956

-

-

?

CTP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate

Enterococcus faecalis

-

-

702284

-

-

?

CTP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate

Escherichia coli

-

CTP specifically required

4406

-

-

?

CTP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate

Escherichia coli

-

CTP specifically required

648960

-

-

r

CTP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate

Homo sapiens

-

human enzyme can use both ATP and CTP with similar affinity. Enzyme shows cooperative binding of ATP, measured as a Hill constant of 1.7

702454

-

-

?

CTP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate

Mammalia

-

CTP specifically required

4406

-

-

?

CTP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate

Micrococcus luteus

-

-

4412

-

4412

?

CTP + (R)-4'-phosphopantothenate + L-Cys

(R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate

Morganella morganii

-

CTP specifically required

4406

-

4406

?

CTP + (R)-4'-phosphopantothenate + L-cysteine

CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine

Drosophila melanogaster

-

-

691514

-

-

?

CTP + (R)-4'-phosphopantothenate + L-cysteine

CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine

Drosophila melanogaster

-

the enzyme is required for various processes that occur during oogenesis including chorion patterning, e.g. for F-actin remodeling during cytoplasmic dumping, overview

691514

-

-

?

CTP + (R)-4'-phosphopantothenate + L-cysteine

CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine

Escherichia coli

-

-

648960

-

-

r

CTP + (R)-4'-phosphopantothenate + L-cysteine

CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine

Methanocaldococcus jannaschii

Q58323

-

674749

-

-

?

CTP + (R)-4'-phosphopantothenate + L-cysteine

CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine

Methanocaldococcus jannaschii

Q58323

detection of the 4'-phosphopantothenoyl-CMP intermediate

674749

-

-

?

CTP + (R)-4'-phosphopantothenate + L-cysteine

CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine

Methanocaldococcus jannaschii DSM 2661

Q58323

-

674749

-

-

?

CTP + (R)-4'-phosphopantothenate + L-cysteine

CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine

Methanocaldococcus jannaschii DSM 2661

Q58323

detection of the 4'-phosphopantothenoyl-CMP intermediate

674749

-

-

?