Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CMP | competitive to CTP, mixed type to (R)-4'-phosphopantothenate, uncompetitive to L-Cys | Enterococcus faecalis | |
L-Cys | - |
Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
(R)-4'-phosphopantothenate | pH 7.6, 37°C | Enterococcus faecalis | |
0.086 | - |
L-Cys | pH 7.6, 37°C | Enterococcus faecalis | |
0.156 | - |
CTP | pH 7.6, 37°C | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28415 | - |
2 * 28415, ESI-MS | Enterococcus faecalis |
60000 | - |
gel filtration | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Enterococcus faecalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine | bi uni uni bi ping pong mechanism, with the nucleotide CTP binding first and CMP released last | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-4'-phosphopantothenate + L-Cys | 8% of the activity with CTP | Enterococcus faecalis | (R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate | - |
? | |
CTP + (R)-4'-phosphopantothenate + L-Cys | - |
Enterococcus faecalis | (R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28415, ESI-MS | Enterococcus faecalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
CTP | pH 7.6, 37°C | Enterococcus faecalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.766 | - |
L-Cys | substrate CTP, pH 7.6, 37°C | Enterococcus faecalis |