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6.1.1.3: threonine-tRNA ligase

This is an abbreviated version!
For detailed information about threonine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.3

Reaction

ATP
+
L-threonine
 +
tRNAThr
=
AMP
 +
diphosphate
 +
L-threonyl-tRNAThr

Synonyms

ApThrRS-1, ApThrRS-2, BaThrRS, EcThrRS, ectRNAThr, McThrRS, mitochondrial threonyl-tRNA synthetase, MJ1197, MmThrRS, More, Mst1, ScmtThrRS, SfThrRS-1, SfThrRS-2, Synthetase, threonyl-transfer ribonucleate, TarS, Thr-tRNA synthetase, Threonine translase, Threonine--tRNA ligase, Threonine-transfer ribonucleate synthetase, threonyl tRNA synthetase, Threonyl-ribonucleic synthetase, Threonyl-transfer ribonucleate synthetase, Threonyl-transfer ribonucleic acid synthetase, Threonyl-transfer RNA synthetase, Threonyl-tRNA synthetase, ThrRS, ThrRS1, ThrS, TRS

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.3 threonine-tRNA ligase

Purification

Purification on EC 6.1.1.3 - threonine-tRNA ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
5SrRNA-L5 protein complex containing both ThrRS and HisRS
-
metal chelate affinity column chromatography
Ni2+ affinity column chromatography
-
overproduced in Escherichia coli
-
recombinant from Escherichia coli
recombinant His-tagged enzyme from Escherichia coli mutant by nickel affinity chromatography
recombinant His-tagged enzyme from strain IBPC6881, to homogeneity
-
recombinant His-tagged mutant H385A
-
recombinant His-tagged ThrRS by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant D-aminoacyl-tRNA deacylase-like domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzyme from Escherichia coli mutant by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant non-tagged N1 and N2 domains comprising residues 1-65 and 66-225 in one fragment by ion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, and ultrafiltration
recombinant residues 1-183 from Escherichia coli strain BL21(DE3) by ion exchange chromatography, ammonium sulfate fractionation and gel filtration to homogeneity
-
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
using a RESOURCE S column
-