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Literature summary for 6.1.1.3 extracted from
- Cloning, expression, purification, crystallization and preliminary X-ray crystallographic investigations of a unique editing domain from archaebacteria (2004), Acta Crystallogr. Sect. D, 60, 1662-1664.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination of residues 1-183, expression in Escherichia coli strain BL21(DE3) | Pyrococcus abyssi |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified ultrafiltrated recombinant residues 1-183, hanging drop vapour diffusion method, protein solution, containing 50 mM Tris-HCl, pH 7.5, and 50 mM NaCl, drops of varying volumes against 0.75 ml reservoir solution, at room temperature or at 4°C, preliminary X-ray diffraction for structure determination at 1.95 A resolution | Pyrococcus abyssi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-threonine + tRNAThr | Pyrococcus abyssi | - |
AMP + diphosphate + L-threonyl-tRNAThr | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus abyssi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant residues 1-183 from Escherichia coli strain BL21(DE3) by ion exchange chromatography, ammonium sulfate fractionation and gel filtration to homogeneity | Pyrococcus abyssi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-threonine + tRNAThr | - |
Pyrococcus abyssi | AMP + diphosphate + L-threonyl-tRNAThr | - |
r | |
| ATP + L-threonine + tRNAThr | editing mechanism | Pyrococcus abyssi | AMP + diphosphate + L-threonyl-tRNAThr | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Threonyl-tRNA synthetase | - |
Pyrococcus abyssi |
| ThrRS | - |
Pyrococcus abyssi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| AMP | - |
Pyrococcus abyssi | |
| ATP | - |
Pyrococcus abyssi | |
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