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6.1.1.3: threonine-tRNA ligase

This is an abbreviated version!
For detailed information about threonine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.3

Reaction

ATP
+
L-threonine
 +
tRNAThr
=
AMP
 +
diphosphate
 +
L-threonyl-tRNAThr

Synonyms

ApThrRS-1, ApThrRS-2, BaThrRS, EcThrRS, ectRNAThr, McThrRS, mitochondrial threonyl-tRNA synthetase, MJ1197, MmThrRS, More, Mst1, ScmtThrRS, SfThrRS-1, SfThrRS-2, Synthetase, threonyl-transfer ribonucleate, TarS, Thr-tRNA synthetase, Threonine translase, Threonine--tRNA ligase, Threonine-transfer ribonucleate synthetase, threonyl tRNA synthetase, Threonyl-ribonucleic synthetase, Threonyl-transfer ribonucleate synthetase, Threonyl-transfer ribonucleic acid synthetase, Threonyl-transfer RNA synthetase, Threonyl-tRNA synthetase, ThrRS, ThrRS1, ThrS, TRS

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.3 threonine-tRNA ligase

Cloned

Cloned on EC 6.1.1.3 - threonine-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
deletion constructs of the ThrRS-dual targeting peptide, ThrRS-dTP, are generated, consisting of amino acid residues 1-50, 1-60, or 24-68, ligated into the pCR Blunt II TOPO vector as a fusion protein with GFP located C-terminally, for a GST-ThrRS-dTP(1-60) construct the vector pGEX-6P-2 is used
-
DNA and amino acid sequence determination of residues 1-183, expression in Escherichia coli strain BL21(DE3)
-
DNA and amino avid sequence analysis
-
DNA sequence analysis
expressed in Escherichia coli BL21-Codon plus cells
-
expressed in Escherichia coli Rosetta pLysS cells
-
expression of His-tagged enzyme in an enzyme-deficient Escherichia coli mutant, complementation analysis
expression of His-tagged ThrRS
-
expression of His-tagged wild-type and mutant enzyme in an enzyme-deficient Escherichia coli mutant, complementation analysis
expression of non-tagged N1 and N2 domains comprising residues 1-65 and 66-225 in one fragment in strain BL21
for expression in Escherichia coli cells
gene LAS, map based cloning of LAS allele, a mapping population is derived from heterozygous plants (LAS/las) crossed with japonica cv. Nipponbare, quantitative real-time RT-PCR expression analysis. The LAS locus is mapped to the short arm of chromosome 2 to a 134 kb genomic DNA region
gene MST1, recombinant expression in Escherichia coli strain BL21(DE3)
gene tars, quantitative real-time PCR expression analysis
gene thrS, expression of wild-type and mutant enzymes in Escherichia coli strain BL21
gene thrS, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene thrS, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene thrS, sequence comparisons, recombinant expression of wild-type enzyme in Escherichia coli strain BL21(DE3)
-
overexpression in Escherichia coli
overexpression in strain IBPC6881 as His-tagged enzyme
-
overexpression of His-tagged mutant enzymes
-
overexpression of His-tagged wild-type and mutant D-aminoacyl-tRNA deacylase-like domains in Escherichia coli strain BL21(DE3), mutant M129K is located in inclusion bodies
overexpression of wild-type and mutants in the null mutant strain
-
the two types of ThrRS from the crenarchaea Aeropyrum pernix and Sulfolobus tokodaii are overexpressed in Eschericha coli