6.1.1.3: threonine-tRNA ligase
This is an abbreviated version!
For detailed information about threonine-tRNA ligase, go to the full flat file.
Word Map on EC 6.1.1.3
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6.1.1.3
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synthetases
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aminoacyl-trna
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aminoacylation
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threonylation
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anticodon
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aarss
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borrelidin
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phenylalanyl-trna
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isoacceptors
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misactivates
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noncognate
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alanyl-trna
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mischarged
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anticodon-binding
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hisrs
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anticodon-like
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mistranslation
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post-transfer
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diagnostics
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drug development
- 6.1.1.3
- synthetases
- aminoacyl-trna
- aminoacylation
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threonylation
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anticodon
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aarss
- borrelidin
- phenylalanyl-trna
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isoacceptors
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misactivates
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noncognate
- alanyl-trna
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mischarged
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anticodon-binding
- hisrs
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anticodon-like
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mistranslation
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post-transfer
- diagnostics
- drug development
Reaction
Synonyms
ApThrRS-1, ApThrRS-2, BaThrRS, EcThrRS, ectRNAThr, McThrRS, mitochondrial threonyl-tRNA synthetase, MJ1197, MmThrRS, More, Mst1, ScmtThrRS, SfThrRS-1, SfThrRS-2, Synthetase, threonyl-transfer ribonucleate, TarS, Thr-tRNA synthetase, Threonine translase, Threonine--tRNA ligase, Threonine-transfer ribonucleate synthetase, threonyl tRNA synthetase, Threonyl-ribonucleic synthetase, Threonyl-transfer ribonucleate synthetase, Threonyl-transfer ribonucleic acid synthetase, Threonyl-transfer RNA synthetase, Threonyl-tRNA synthetase, ThrRS, ThrRS1, ThrS, TRS
ECTree
Advanced search results
Turnover Number
Turnover Number on EC 6.1.1.3 - threonine-tRNA ligase
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1.83
L-serine
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in 100 mM Na-HEPES (pH 7.2), 30 mM KCl, 10 mM MgCl2, 2 mM potassium fluoride, at 37°C
1.9
L-threonine
pH 7.2, 60°C, recombinant wild-type enzyme
3.32
L-threonine
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in 100 mM Na-HEPES (pH 7.2), 30 mM KCl, 10 mM MgCl2, 2 mM potassium fluoride, at 37°C
13.5
L-threonine
pH 7.2, 60°C, recombinant wild-type enzyme
0.033
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mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.05
tRNA1Thr
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mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.05
tRNA1Thr
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mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.05
tRNA1Thr
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mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.05
tRNA1Thr
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wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.053
tRNA1Thr
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mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.055
tRNA1Thr
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mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.06
tRNA1Thr
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mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.067
tRNA1Thr
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mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.073
tRNA1Thr
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mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.08
tRNA1Thr
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mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.082
tRNA1Thr
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mutant enzyme Y405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.087
tRNA1Thr
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mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.093
tRNA1Thr
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mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.112
tRNA1Thr
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mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.117
tRNA1Thr
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mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.118
tRNA1Thr
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mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.01
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mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.017
tRNA2Thr
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mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.03
tRNA2Thr
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mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.038
tRNA2Thr
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wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.04
tRNA2Thr
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mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.043
tRNA2Thr
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mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.045
tRNA2Thr
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mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.045
tRNA2Thr
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mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.053
tRNA2Thr
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mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.058
tRNA2Thr
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mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.06
tRNA2Thr
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mutant enzyme Y405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.067
tRNA2Thr
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mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.088
tRNA2Thr
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mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.1
tRNA2Thr
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mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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0.103
tRNA2Thr
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mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication
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additional information
additional information
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mutant enzymes complementing the null mutant
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additional information
additional information
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rate constants for adenylate synthesis by ThrRS in the absence of tRNA, overview
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