Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Codon plus cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D423A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
D437A | the mutant shows strongly increased catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
E401A | the mutant shows strongly increased catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
K408A | the mutant shows about wild type catalytic efficiency for tRNAThr1 | Saccharomyces cerevisiae |
K440A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
N356A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
N359A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
N400A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
N432A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Saccharomyces cerevisiae |
N432A | the mutant shows weaker binding for tRNAThr1 and displays increased Km for tRNAThr2 as well | Saccharomyces cerevisiae |
Q362A | the mutant displays increased Km values for tRNAThr2 | Saccharomyces cerevisiae |
R434A | the mutant shows weaker binding for tRNAThr1 only | Saccharomyces cerevisiae |
R439A | the mutant shows weaker binding for tRNAThr1 and displays increased Km for tRNAThr2 as well | Saccharomyces cerevisiae |
S409E | the mutant shows about wild type catalytic efficiency for tRNAThr1 | Saccharomyces cerevisiae |
T357A | the mutant displays increased Km values for tRNAThr2 | Saccharomyces cerevisiae |
Y405A | the mutant displays increased Km values for tRNAThr2 | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
tRNA1Thr | mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.0002 | - |
tRNA1Thr | mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00024 | - |
tRNA1Thr | mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00024 | - |
tRNA1Thr | mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00027 | - |
tRNA2Thr | mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00028 | - |
tRNA1Thr | mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00029 | - |
tRNA1Thr | wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.0003 | - |
tRNA1Thr | mutant enzyme E405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.0003 | - |
tRNA1Thr | mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00031 | - |
tRNA1Thr | mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00031 | - |
tRNA1Thr | mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00037 | - |
tRNA2Thr | mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00044 | - |
tRNA2Thr | mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00044 | - |
tRNA2Thr | wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00046 | - |
tRNA1Thr | mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00047 | - |
tRNA1Thr | mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00048 | - |
tRNA1Thr | mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00049 | - |
tRNA1Thr | mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00049 | - |
tRNA2Thr | mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00051 | - |
tRNA2Thr | mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00059 | - |
tRNA2Thr | mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00059 | - |
tRNA1Thr | mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00064 | - |
tRNA2Thr | mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00069 | - |
tRNA2Thr | mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00078 | - |
tRNA2Thr | mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00083 | - |
tRNA1Thr | mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00083 | - |
tRNA2Thr | mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00094 | - |
tRNA2Thr | mutant enzyme E405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00095 | - |
tRNA1Thr | mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00123 | - |
tRNA2Thr | mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00139 | - |
tRNA2Thr | mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.0014 | - |
tRNA2Thr | mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains a Zn2+ ion | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNA1Thr | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-threonyl-tRNA1Thr | - |
? | |
ATP + L-threonine + tRNA2Thr | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-threonyl-tRNA2Thr | - |
? | |
ATP + L-threonine + tRNAThr | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNA1Thr | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-threonyl-tRNA1Thr | - |
? | |
ATP + L-threonine + tRNA2Thr | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-threonyl-tRNA2Thr | - |
? | |
ATP + L-threonine + tRNAThr | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Mst1 | - |
Saccharomyces cerevisiae |
Threonyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
tRNA2Thr | mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.017 | - |
tRNA2Thr | mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.03 | - |
tRNA2Thr | mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.033 | - |
tRNA1Thr | mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.038 | - |
tRNA2Thr | wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.04 | - |
tRNA2Thr | mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.043 | - |
tRNA2Thr | mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.045 | - |
tRNA2Thr | mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.045 | - |
tRNA2Thr | mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.05 | - |
tRNA1Thr | mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.05 | - |
tRNA1Thr | mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.05 | - |
tRNA1Thr | mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.05 | - |
tRNA1Thr | wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.053 | - |
tRNA1Thr | mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.053 | - |
tRNA2Thr | mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.055 | - |
tRNA1Thr | mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.058 | - |
tRNA2Thr | mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.06 | - |
tRNA1Thr | mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.06 | - |
tRNA2Thr | mutant enzyme Y405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.067 | - |
tRNA1Thr | mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.067 | - |
tRNA2Thr | mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.073 | - |
tRNA1Thr | mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.08 | - |
tRNA1Thr | mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.082 | - |
tRNA1Thr | mutant enzyme Y405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.087 | - |
tRNA1Thr | mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.088 | - |
tRNA2Thr | mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.093 | - |
tRNA1Thr | mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.1 | - |
tRNA2Thr | mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.103 | - |
tRNA2Thr | mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.112 | - |
tRNA1Thr | mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.117 | - |
tRNA1Thr | mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.118 | - |
tRNA1Thr | mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |