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5.3.99.3: prostaglandin-E synthase

This is an abbreviated version!
For detailed information about prostaglandin-E synthase, go to the full flat file.

Word Map on EC 5.3.99.3

Reaction

(13E)-(15S)-15-hydroxy-9-oxoprosta-10,13-dienoate
=
(13E)-(15S)-15-hydroxy-9-oxoprosta-11,13-dienoate

Synonyms

CPGES, cPGES/p23, cytosolic prostaglandin E synthase, cytosolic prostaglandin E(2) synthase, cytosolic prostaglandin E(2) synthase-2, endoperoxide isomerase, Isomerase, prostaglandin R2 E-, membrane-associated PGE2 synthase, membrane-associated prostaglandin E synthase-2, membrane-associated prostaglandin E(2) synthase-1, membrane-associated prostaglandin E(2) synthase-2, microsomal PG synthase-1, microsomal PGE synthase-1, microsomal PGE2 synthase, microsomal PGE2 synthase-1, microsomal prostaglandin E synthase, microsomal prostaglandin E synthase 1, microsomal prostaglandin E synthase type 1, microsomal prostaglandin E synthase type 2, microsomal prostaglandin E synthase type-2, microsomal prostaglandin E synthase-1, microsomal prostaglandin E synthase-2, microsomal prostaglandin E(2) synthase-1, microsomal prostaglandin E(2) synthase-2, microsomal prostaglandin E2 synthase 1, microsomal prostaglandin E2 synthase type 1, microsomal prostaglandin E2 synthase-1, microsomal prostaglandin synthase-1, microsomal prostaglandin-E synthase-1, mPGE synthase-2, mPGES, mPGES-1, mPGES-2, MPGES1, MPGES2, p23, PGE, PGE isomerase, PGE synthase, PGE synthase-1, PGE2, PGE2 isomerase, PGE2 synthase, PGE2-synthase, PGES, PGES-1, PGES-2, PGH-PGE isomerase, PGH2/PGE2 isomerase, prostagandin-E synthetase, Prostaglandin E synthase, prostaglandin E synthase 1, prostaglandin E synthase type 2, prostaglandin E synthase-1, prostaglandin E synthase-2, Prostaglandin E2 isomerase, prostaglandin E2 synthase, prostaglandin E2 synthase-1, prostaglandin E2 synthase-I, Prostaglandin endoperoxide E isomerase, Prostaglandin endoperoxide E2 isomerase, Prostaglandin endoperoxide-E isomerase, Prostaglandin H-E isomerase, Prostaglandin H-prostaglandin E isomerase, Prostaglandin R-prostaglandin E isomerase, prostaglandin synthase-1, prostaglandin-E synthase, Prostaglandin-H2 E-isomerase, PTGES

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.99 Other intramolecular oxidoreductases
                5.3.99.3 prostaglandin-E synthase

Engineering

Engineering on EC 5.3.99.3 - prostaglandin-E synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D97A
-
site-directed mutagenesis, isomerase inactive mutant
N96A
-
site-directed mutagenesis, the mutant shows reduced isomerase activity compared to the wild-type enzyme
R99A
-
site-directed mutagenesis, isomerase inactive mutant
D49A
inactive
D49N
the mutant shows severely reduced activity compared to the wild type enzyme
E66A
mutant, fraction of wild-type activity, 53%
E77A
mutant, no activity
N74A
the mutant shows increased Km compared to the wild type enzyme
Q134E
-
fivefold increase in Km value
Q36E
-
tenfold increase in Km value
R110A
mutant, no activity
R110S
mutant, no activity
R110T
-
18% of wild-type activity
R122A
the mutant shows increased Km compared to the wild type enzyme
R126A
R126K
the mutant shows severely reduced activity compared to the wild type enzyme
R126L
inactive
R126Q
R67A
mutant, no activity
R70A/R122A
the mutant shows increased Km compared to the wild type enzyme, the double mutant of the mPGES-1 significantly reduces the binding affinity of GSH
R70A/Y117A
mutant, fraction of wild-type activity, 7%
R70S
mutant, full activity
R73A
the mutant retains 50% activity compared to the wild type enzyme
R73L
inactive
S127A
S127C
the mutant displays 80% lowered activity
T114V
-
21% of wild-type activity
T129V
the mutant shows increased Km compared to the wild type enzyme
T131V/L135F/A138F
-
the mutant shows bout 4fold reduced specific activity towards prostaglandin H2 compared to the wild type enzyme and is not inhibited by 1-C-(2-chlorobenzene)-3-N-(naphthalen-1-yl)benzene-1,3-dicarboxamide
Y117A
Y117F
Y117S
the mutant shows increased Km compared to the wild type enzyme
Y130I
A151G
-
shows similar activity as the wild-type
C-delta-35
-
35-amino acid COOH-terminal deletion, diminishes association of the enzyme with membranes
C58K
-
diminishes association of the enzyme with membranes
S113A
-
fails to be activated by casein kinase II
S118A
-
fails to be activated by casein kinase II
V131T/F135L/F138A
-
the mutant shows bout 4fold increased specific activity towards prostaglandin H2 compared to the wild type enzyme and is inhibited by 1-C-(2-chlorobenzene)-3-N-(naphthalen-1-yl)benzene-1,3-dicarboxamide
Y14F
-
shows similar activity as the wild-type
C-delta-35
-
35-amino acid COOH-terminal deletion, diminishes association of the enzyme with membranes
C58K
-
diminishes association of the enzyme with membranes
additional information