Literature summary for 5.3.99.3 extracted from

Raouf, J.; Rafique, N.; Goodman, M.; Idborg, H.; Bergqvist, F.; Armstrong, R.; Jakobsson, P.; Morgenstern, R.; Spahiu, L.
Arg126 and Asp49 are essential for the catalytic function of microsomal prostaglandin E2 synthase 1 and Ser127 is not (2016), PLoS ONE, 11, e163600 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21Star (DE) pLysS cells and SF9 insect cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D49A	inactive	Homo sapiens
R126A	inactive	Homo sapiens
R126L	inactive	Homo sapiens
R73A	the mutant retains 50% activity compared to the wild type enzyme	Homo sapiens
R73L	inactive	Homo sapiens
S127A	the mutant retains 63% activity compared to the wild type enzyme	Homo sapiens
S127C	the mutant displays 80% lowered activity	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Homo sapiens	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Prostaglandin H2	Homo sapiens	-	Prostaglandin E2	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O14684	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Sephacryl gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Prostaglandin H2	-	Homo sapiens	Prostaglandin E2	-	?

Subunits

Subunits	Comment	Organism
?	x * 17000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
microsomal prostaglandin E synthase 1	-	Homo sapiens
MPGES1	-	Homo sapiens

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Release 2023.1 (January 2023)