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5.3.99.3: prostaglandin-E synthase

This is an abbreviated version!
For detailed information about prostaglandin-E synthase, go to the full flat file.

Word Map on EC 5.3.99.3

Reaction

(13E)-(15S)-15-hydroxy-9-oxoprosta-10,13-dienoate
=
(13E)-(15S)-15-hydroxy-9-oxoprosta-11,13-dienoate

Synonyms

CPGES, cPGES/p23, cytosolic prostaglandin E synthase, cytosolic prostaglandin E(2) synthase, cytosolic prostaglandin E(2) synthase-2, endoperoxide isomerase, Isomerase, prostaglandin R2 E-, membrane-associated PGE2 synthase, membrane-associated prostaglandin E synthase-2, membrane-associated prostaglandin E(2) synthase-1, membrane-associated prostaglandin E(2) synthase-2, microsomal PG synthase-1, microsomal PGE synthase-1, microsomal PGE2 synthase, microsomal PGE2 synthase-1, microsomal prostaglandin E synthase, microsomal prostaglandin E synthase 1, microsomal prostaglandin E synthase type 1, microsomal prostaglandin E synthase type 2, microsomal prostaglandin E synthase type-2, microsomal prostaglandin E synthase-1, microsomal prostaglandin E synthase-2, microsomal prostaglandin E(2) synthase-1, microsomal prostaglandin E(2) synthase-2, microsomal prostaglandin E2 synthase 1, microsomal prostaglandin E2 synthase type 1, microsomal prostaglandin E2 synthase-1, microsomal prostaglandin synthase-1, microsomal prostaglandin-E synthase-1, mPGE synthase-2, mPGES, mPGES-1, mPGES-2, MPGES1, MPGES2, p23, PGE, PGE isomerase, PGE synthase, PGE synthase-1, PGE2, PGE2 isomerase, PGE2 synthase, PGE2-synthase, PGES, PGES-1, PGES-2, PGH-PGE isomerase, PGH2/PGE2 isomerase, prostagandin-E synthetase, Prostaglandin E synthase, prostaglandin E synthase 1, prostaglandin E synthase type 2, prostaglandin E synthase-1, prostaglandin E synthase-2, Prostaglandin E2 isomerase, prostaglandin E2 synthase, prostaglandin E2 synthase-1, prostaglandin E2 synthase-I, Prostaglandin endoperoxide E isomerase, Prostaglandin endoperoxide E2 isomerase, Prostaglandin endoperoxide-E isomerase, Prostaglandin H-E isomerase, Prostaglandin H-prostaglandin E isomerase, Prostaglandin R-prostaglandin E isomerase, prostaglandin synthase-1, prostaglandin-E synthase, Prostaglandin-H2 E-isomerase, PTGES

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.99 Other intramolecular oxidoreductases
                5.3.99.3 prostaglandin-E synthase

Crystallization

Crystallization on EC 5.3.99.3 - prostaglandin-E synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified enzyme in complex with glutathione sulfonic acid, sitting drop vapor diffusion method, mixing of 10 mg/ml protein in 20 mM Tris-HCl , pH 8.5, and 0.2 M NaCl with 0.1 M HEPES, pH 7.5, 30% PEG 400 w/v, and 20% 1,2-propanediol v/v, in a 1:1 or 1:2 ratio, X-ray diffraction structure determination and analysis at 1.37 A resolution, molecular replacement
-
purified recombinant microsomal isozyme PGE2 synthase-1, in presence of beta-octyl-glycoside and GSH or 1-(4-phenylphenyl)-2-(S-glutathionyl)-ethanone (bis-phenyl-GSH), hanging drop vapor diffusion method, the reservoir solutions used contain 100 mM CAPSO, pH 9.5, 30% PEG 400, 100 mM NaCl, and 100 mM Li2SO4 (mersalyl acid soak and bis-phenyl-GSH complex) or 100 mM Tris, pH 8.0-8.5, 30% PEG400, 100 mM NaCl, and 1 mM TCEP (native GSH complex), 4°C, X-ray diffraction structure determination and analysis at 1.2 A resolution
red-coloured protein purified after growth of Escherichia coli in LB medium containing delta-aminolevulinate and Fe(NO3)3. Enzyme contains bound glutathione and heme
-
the structure of human MPGES1 is determined in complex with the tripeptide gamma-L-glutamyl-L-cysteinyl-glycine, glutathione, at 3.5 A in-plane resolution using electron crystallography
three-dimensional model of substrate-domain and its binding with subsrtates prostaglandin H2 and glutathione. Residue Y130 plays a key role in binding with prostaglandin H2 and in the catalytic process. R110 and T114 interact intensively with the carbonyl tail of prostaglandin H2, whereas Q36 and Q134 only enhance the prostaglandin H2 binding affinity. Prostaglandin H2 interacts with glutathione through hydrogen binding between the peroxy group of prostaglandin H2 and the -SH group of glutathione
-
threedimensional structural model of binding with inhibitors, quantitative structure-activity correlation
-
purified recombinant His-tagged truncated mPGES-2, complexed with anti-inflammatory drug IMN, 10 mg/ml protein in 1.7 M ammonium sulfate, 0.1 M sodium acetate-HCl, pH 5.5, and 1.5 mM indomethacin, sitting drop vapour diffusion method, 3 days, 22°C, X-ray diffraction structure determinationand analysis at 2.6 A resolution, modeling
with an anti-inflammatory drug indomethacin, 2.6 A resolution