Cloned (Comment) | Organism |
---|---|
expression of N-terminally truncated enzyme in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
red-coloured protein purified after growth of Escherichia coli in LB medium containing delta-aminolevulinate and Fe(NO3)3. Enzyme contains bound glutathione and heme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoform mPGES-2 | - |
Purification (Comment) | Organism |
---|---|
purification after expression of N-terminally truncated enzyme in recombinant Escherichia coli growing in LB medium containing delta-aminolevulinate and Fe(NO3)3 gives a protein of red colour. Purification after growth of Escherichia coli on minimal medium gives a colourless protein | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | glutathione-heme-complex bound enzyme catalyzes formation of 12(S)-hydroxy-5(Z),8(E),10(E)-heptadecatrienoic acid and malonaldehyde from prostaglandin H2, i.e. shows lyase activity | Homo sapiens | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | crystallization data of red-coloured protein purified after growth of recombinant Escherichia coli in LB medium containing delta-aminolevulinate and Fe(NO3)3. Heme is complexed with bound glutathione forming a S-Fe coordination bond with no polar interaction with enzyme. Heme dissociation constant is 0.00053 mM | Homo sapiens |