Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Macaca fascicularis |
overexpression of truncated mPGES-2, comprising residues 1-87, with an extended His-tag in Escherichia coli strain BL21 by nickel affinity chromatography | Macaca fascicularis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged truncated mPGES-2, complexed with anti-inflammatory drug IMN, 10 mg/ml protein in 1.7 M ammonium sulfate, 0.1 M sodium acetate-HCl, pH 5.5, and 1.5 mM indomethacin, sitting drop vapour diffusion method, 3 days, 22°C, X-ray diffraction structure determinationand analysis at 2.6 A resolution, modeling | Macaca fascicularis |
with an anti-inflammatory drug indomethacin, 2.6 A resolution | Macaca fascicularis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Macaca fascicularis | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Prostaglandin H2 | Macaca fascicularis | - |
Prostaglandin E2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macaca fascicularis | Q9N0A4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Macaca fascicularis |
recombinant His-tagged truncated mPGES-2 from Escherichia coli strain BL21 by nickel affinity chromatography | Macaca fascicularis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate | catalytic mechanism of mPGES-2, hydrogen bond-forming residues Y107, C113, C110, and F112 are involved, substrate binding and catalytic sites | Macaca fascicularis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Prostaglandin H2 | - |
Macaca fascicularis | Prostaglandin E2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystal structure | Macaca fascicularis |
More | structure-function analysis, overview | Macaca fascicularis |
Synonyms | Comment | Organism |
---|---|---|
microsomal prostaglandin E synthase type 2 | - |
Macaca fascicularis |
mPGES-2 | - |
Macaca fascicularis |
prostaglandin E synthase type 2 | - |
Macaca fascicularis |