5.3.1.4: L-arabinose isomerase
This is an abbreviated version!
For detailed information about L-arabinose isomerase, go to the full flat file.
Word Map on EC 5.3.1.4
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5.3.1.4
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d-tagatose
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isomerization
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l-ribulose
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nutrition
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geobacillus
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sweetener
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isomerases
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synthesis
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stearothermophilus
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food industry
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thermodenitrificans
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low-calorie
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l-ribulokinase
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packed-bed
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arabad
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sakei
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4-epimerase
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industry
- 5.3.1.4
- d-tagatose
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isomerization
- l-ribulose
- nutrition
- geobacillus
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sweetener
- isomerases
- synthesis
- stearothermophilus
- food industry
- thermodenitrificans
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low-calorie
- l-ribulokinase
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packed-bed
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arabad
- sakei
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4-epimerase
- industry
Reaction
Synonyms
AI, AraA, arabinose isomerase, D-galactose isomerase, ECAI, gali 152, gali 153, GSAI, GSAI 152, GSAI 153, Isomerase, L-arabinose, L-AI, L-AI NC8, L-AI US100, L-arabinose aldose-ketose-isomerase, L-arabinose isomerase, L-arabinose isomerase 1, L-arabinose isomerase 2, L-arabinose ketol-isomerase, pL 151, pL 152, pL 153, pL151, TNAI
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Application on EC 5.3.1.4 - L-arabinose isomerase
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food industry
industry
nutrition
synthesis
additional information
food industry
production of D-tagatose as a low-calorie sugar-substituting sweetener
food industry
production of D-tagatose as a low-calorie sugar-substituting sweetener
food industry
production of D-tagatose as a low-calorie sugar-substituting sweetener
food industry
production of D-tagatose as a low-calorie sugar-substituting sweetener lower pH is preferable for industrial production
food industry
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production of D-tagatose as a low-calorie sugar-substituting sweetener, reactor was run for 50 days
food industry
production of D-tagatose as a low-calorie sugar-substituting sweetener, the D-tagatose yield from the mutated enzyme is higher than from the wild type
food industry
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hyperthermophilic L-arabinose isomerase is useful in the commercial production of D-tagatose as a low-calorie bulk sweetener
food industry
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hyperthermophilic L-arabinose isomerase is useful in the commercial production of D-tagatose as a low-calorie bulk sweetener
food industry
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production of D-tagatose as a low-calorie sugar-substituting sweetener
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food industry
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production of D-tagatose as a low-calorie sugar-substituting sweetener, the D-tagatose yield from the mutated enzyme is higher than from the wild type
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a novel way of producing L-ribose from the readily available raw material L-arabinose is described
industry
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a novel way of producing L-ribose from the readily available raw material L-arabinose is described
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the enzyme may be a good model for analysis of metal-mediated thermostabilization and for industrial application in the production of D-tagatose as a novel sweetener
nutrition
AI from Bacillus thermodenitrificans can be useful for the industrial production of D-tagatose because its optimum temperature is the highest reported for thermophilic AIs and because its activity does not require the addition of Co2+
nutrition
the product D-tagatose is currently introduced as a low-calorie bulk sweetener
nutrition
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the product D-tagatose is used as a low-calorie bulk sweetener
nutrition
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there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
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there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
Lactobacillus gayonii
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there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
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there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
-
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
-
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
Halalkalibacterium halodurans
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
-
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
nutrition
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when mice are fed with a lactobacillus lactis strain secreting the enzyme and galactose, tagatose is produced in vivo and reduces the glycemia index
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an Escherichia coli galactose kinase gene knockout strain, which contains the L-arabinose isomerase gene to isomerize D-galactose to D-tagatose, shows a higher conversion yield of tagatose because galactose is not metabolized by endogenous galactose kinase. In whole cells of the galactose kinase knockout strain, the isomerase-catalyzed reaction exhibits an equilibrium shift towards tagatose, producing a tagatose fraction of 68% at 37°C, whereas the purified L-arabinose isomerase gives a tagatose equilibriumfraction of 36%
synthesis
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expression of the Escherichia coli genes araA, araB, and araD encoding L-arabinose isomerase, L-ribulokinase, and L-ribulose-5-phosphate 4-epimerase, respectively, in Corynebacterium glutamicum under the control of a constitutive promoter. The recombinant strain is able to grow on mineral salts medium containing L-arabinose as the sole carbon and energy source. Under oxygen deprivation and with L-arabinose as the sole carbon and energy source, carbon flow of the recombinant strain is redirected to produce up to 40, 37, and 11%, respectively, of the theoretical yields of succinic, lactic, and acetic acids. Usinga sugar mixture containing 5% D-glucose and 1% L-arabinose under oxygen deprivation, cells metabolize L-arabinose at a constant rate, resulting in combined organic acids yield based on the amount of sugar mixture consumed after D-glucose depletion of 83% that is comparable to that before D-glucose depletion, 89%
synthesis
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production of D-tagatose from D-galactose by L-arabinose isomerase immobilized on chitopearl beads. Half-lives of immobilized enzyme at 70°C, 75°C, 80°C, 85°C and 90°C are 388, 106, 54, 36, and 22 h, respectively. With pH control at 7.5, D-tagatose production at 70°C in a stirred tank reactor doubles compared to conditions without pH control
synthesis
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production of ethanol after improvement of a bacterial L-arabinose utilization pathway consisting of L-arabinose isomerase from Bacillus licheniformis and L-ribulokinase and L-ribulose-5-phosphate-4-epimerase from Escherichia coli after expression of the corresponding genes in Saccharomyces cerevisiae.Yeast transformants expressing the codon-optimized genes show strongly improved L-arabinose conversion rates. The ethanol production rate from L-arabinose can be increased more than 2.5fold from 0.014 g ethanol per h and g dry weight to 0.036 g ethanol per h and g dry weight and the ethanol yield can be increased from 0.24 g ethanol per g consumed L-arabinose to 0.39 g ethanol per g consumed L-arabinose
synthesis
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production of the intracellular enzymes L-arabinose isomerase and D-xylose isomerase in Lactobacillus bifermentans. After 9 h cultivation in optimized medium, Arabinose isomerase and xylose isomerase activities are 9.4 and 7.24 U/ml, respectively. For optimal growth, the strain requires Tween 80 at 1 g/l and a source of inorganic nitrogen, e.g., ammonium citrate. The bacterium has no requirement for sodium acetate for either growth or production of isomerases. The production rate of enzymes is increased when metal ions are added, primarily manganese
synthesis
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strain carrying mutant C450S/N475K is able to produce 95 g L-ribulose per l from 500 g L-arabinose per l under optimum conditions of pH 8, 70°C, and 10 units enzyme per ml with a conversion yield of 19% over 2 h. The half-lives of the mutated enzyme at 70 and 75°C are 35 and 4.5 h, respectively
synthesis
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alginate-immobilized Escherichia coli cells, recombinantly expressing the L-arabinose isomerase from Bacillus licheniformis, shows high stability and are suitable for L-ribulose production at low costs
synthesis
engineered enzyme mutants are useful for production of D-tagatose
synthesis
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L-ribose production of the enzyme in a coupled assay system with mannose-6-phosphate isomerase, EC 5.3.1.8, in recombinant Escherichia coli ER2566, AI/MPI ratio, 1:2.5, method optimization. L-Ribose is a potential starting material for the synthesis of many L-nucleoside-based pharmaceutical compounds
synthesis
the enzyme is useful in production of low-calorie sweetener D-tagatose. Tagatose also may potentially be useful as a prescription drug additive, to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavored lipstick
synthesis
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the enzyme is a biocatalyst for the production of L-ribulose
synthesis
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the enzyme is used for the commercial production of D-tagatose
synthesis
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coexpression of beta-D-galactosidase gene and L-arabinose isomerase mutant Q299K for synthesis of D-tagatose. Recombinant cells exhibit maximum D-tagatose producing activity at 34°C and pH 6.5 and in the presence of borate, 10 mM Fe2+, and 1 mM Mn2+. Cells can hydrolyze more than 95% lactose and convert 43% D-galactose into D-tagatose
synthesis
coexpression with a thermostable beta-galactosidase from Thermus thermophilus HB27 in Escherichia coli. The recombinant cells show optimal catalytic temperature and pH at 70°C and 7.0, respectively. With the addition of borate, D-tagatose is produced directly from lactose in 16 h in a concentration of 101 g/l, a yield of 20.2%, and a productivity of 6.3 g/l/h
synthesis
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enzyme is displayed on the spore surface of Bacillus subtilis DB403 by using an anchoring protein and a peptide linker. This displayed protein shows high specific activity and stability and is used as a immobilized biocatalyst for producing D-tagatose through batch and semi-continuous biotransformation. The conversion rate of D-tagatose from 125 g/l D-galactose achieved 79.7% at 28 h, and the volumetric productivity reaches 4.3 g/l/h at 20 h with good reusability
synthesis
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expression of L-arabinose isomerase in fusion with the signal peptide of usp45 leads to secretion of the enzyme in induced cultures. Secretion is imrpoved by use of Lactobacillus lactis strains deficient for major proteases, ClpP and HtrA, or by use of an enhancer of protein secretion in Lactobacillus lactis fused to the recombinant isomerase gene fused to the signal peptide
synthesis
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production of ribose by immobilized recombinant Escherichia coli cells expressing the L-arabinose isomerase gene and mannose-6-phosphate isomerase mutant W17Q/N90A/L129F. The immobilized cells produce 99 g/l L-ribose from 300 g/l L-arabinose in 3 h at pH 7.5 and 60 °C in the presence of 1 mM Co2+, with a conversion yield of 33 % (w/w) and a productivity of 33 g/l/h
synthesis
under optimal conditions, recombinant Escherichia coli cells expressing AraA can convert 150 g/l and 250 g/l D-galactose to D-tagatose with conversion rates of 32% (32 h) and 27% (48 h)
synthesis
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the enzyme is used for the commercial production of D-tagatose
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synthesis
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enzyme is displayed on the spore surface of Bacillus subtilis DB403 by using an anchoring protein and a peptide linker. This displayed protein shows high specific activity and stability and is used as a immobilized biocatalyst for producing D-tagatose through batch and semi-continuous biotransformation. The conversion rate of D-tagatose from 125 g/l D-galactose achieved 79.7% at 28 h, and the volumetric productivity reaches 4.3 g/l/h at 20 h with good reusability
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synthesis
Weizmannia coagulans NL01
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under optimal conditions, recombinant Escherichia coli cells expressing AraA can convert 150 g/l and 250 g/l D-galactose to D-tagatose with conversion rates of 32% (32 h) and 27% (48 h)
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synthesis
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engineered enzyme mutants are useful for production of D-tagatose
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synthesis
Limosilactobacillus fermentum CGMCC2921
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coexpression with a thermostable beta-galactosidase from Thermus thermophilus HB27 in Escherichia coli. The recombinant cells show optimal catalytic temperature and pH at 70°C and 7.0, respectively. With the addition of borate, D-tagatose is produced directly from lactose in 16 h in a concentration of 101 g/l, a yield of 20.2%, and a productivity of 6.3 g/l/h
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additionally, D-tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
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additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
Lactobacillus gayonii
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additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
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additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
-
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
-
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
Halalkalibacterium halodurans
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
-
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick