Literature summary for 5.3.1.4 extracted from

Yeom, S.J.; Ji, J.H.; Yoon, R.Y.; Oh, D.K.
L-Ribulose production from L-arabinose by an L-arabinose isomerase mutant from Geobacillus thermodenitrificans (2008), Biotechnol. Lett., 30, 1789-1793.

Search for more literature for 5.3.1.4

Application

Application	Comment	Organism
synthesis	strain carrying mutant C450S/N475K is able to produce 95 g L-ribulose per l from 500 g L-arabinose per l under optimum conditions of pH 8, 70°C, and 10 units enzyme per ml with a conversion yield of 19% over 2 h. The half-lives of the mutated enzyme at 70 and 75°C are 35 and 4.5 h, respectively	Geobacillus thermodenitrificans

Protein Variants

Protein Variants	Comment	Organism
C450S/N475K	mutant strain is able to produce 95 g L-ribulose per l from 500 g L-arabinose per l under optimum conditions of pH 8, 70°C, and 10 units enzyme per ml with a conversion yield of 19% over 2 h. The half-lives of the mutated enzyme at 70 and 75°C are 35 and 4.5 h, respectively	Geobacillus thermodenitrificans

Organism

Organism	UniProt	Comment	Textmining
Geobacillus thermodenitrificans	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-arabinose	equilibrium ratio of L-arabinose to L-ribulose is 88.8:11.2 at 25°C, 80.0:19.0 at 55°C, and 78.0:22.0 at 75°C	Geobacillus thermodenitrificans	L-ribulose	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	half-life 35 h	Geobacillus thermodenitrificans
75	-	half-life 4.5 h	Geobacillus thermodenitrificans

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Release 2023.1 (January 2023)