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Literature summary for 5.3.1.4 extracted from
- Characterization of L-arabinose isomerase in Bacillus subtilis, a GRAS host, for the production of edible tagatose (2009), Food Biotechnol., 23, 8-16.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is useful in production of low-calorie sweetener D-tagatose. Tagatose also may potentially be useful as a prescription drug additive, to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavored lipstick | Geobacillus stearothermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene araA, subcloning in Escherichia coli strain DH5alpha, expression in Bacillus subtilis strain 168, ATCC 27370, and secretion at large quantities to the medium | Geobacillus stearothermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | evaluation and optimization of Escherichia coli and Bacilus subtilis expression systems for toxic byproducts in L-ribulose and/or D-tagatose production by the recombinant engineered enzyme | Geobacillus stearothermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 77 | - |
L-arabinose | pH 8.0, 60°C, recombinant enzyme expressed in Bacillus subtilis | Geobacillus stearothermophilus |  |
| 100 | - |
L-arabinose | pH 8.0, 60°C, recombinant enzyme expressed in Escherichia coli | Geobacillus stearothermophilus | |
| 279 | - |
D-galactose | pH 8.0, 60°C, recombinant enzyme expressed in Bacillus subtilis | Geobacillus stearothermophilus | |
| 578 | - |
D-galactose | pH 8.0, 60°C, recombinant enzyme expressed in Escherichia coli | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, SDS-PAGE | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arabinose | Geobacillus stearothermophilus | - |
L-ribulose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | Q9S467 | i.e. Geobacillus stearothermophilus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 18fold from Bacillus subtilis | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 69 | - |
purified recombinant enzyme expressed in Bacillus subtilis, D-galactose isomerization | Geobacillus stearothermophilus |
| 97 | - |
purified recombinant enzyme expressed in Bacillus subtilis, L-arabinose isomerization | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | - |
Geobacillus stearothermophilus | D-tagatose | - |
? | |
| L-arabinose | - |
Geobacillus stearothermophilus | L-ribulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant enzyme expressed in Escherichia coli or Bacillus subtilis, D-galactose isomerization and L-arabinose isomerization | Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | recombinant enzyme expressed in Bacillus subtilis | Geobacillus stearothermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20.18 | - |
D-galactose | pH 8.0, 60°C, recombinant enzyme expressed in Escherichia coli | Geobacillus stearothermophilus | |
| 53.08 | - |
D-galactose | pH 8.0, 60°C, recombinant enzyme expressed in Bacillus subtilis | Geobacillus stearothermophilus | |
| 75.25 | - |
L-arabinose | pH 8.0, 60°C, recombinant enzyme expressed in Bacillus subtilis | Geobacillus stearothermophilus | |
| 107.9 | - |
L-arabinose | pH 8.0, 60°C, recombinant enzyme expressed in Escherichia coli | Geobacillus stearothermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant enzyme expressed in Escherichia coli or Bacillus subtilis, D-galactose isomerization and L-arabinose isomerization | Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | recombinant enzyme expressed in Bacillus subtilis | Geobacillus stearothermophilus |
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