Application | Comment | Organism |
---|---|---|
food industry | production of D-tagatose as a low-calorie sugar-substituting sweetener | Lactiplantibacillus plantarum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lactiplantibacillus plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43.4 | - |
L-arabinose | at 60°C, pH 7.5 | Lactiplantibacillus plantarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required for maximal activity and stability | Lactiplantibacillus plantarum | |
Cu2+ | inhibitory | Lactiplantibacillus plantarum | |
Mn2+ | required for maximal activity and stability | Lactiplantibacillus plantarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Lactiplantibacillus plantarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | A9J246 | NC8 | - |
Lactiplantibacillus plantarum NC8 | A9J246 | NC8 | - |
Purification (Comment) | Organism |
---|---|
of the recombinant wild type and mutant enzymes | Lactiplantibacillus plantarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
263.4 | - |
after purification to homogeneity | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | - |
Lactiplantibacillus plantarum | D-tagatose | - |
? | |
D-galactose | - |
Lactiplantibacillus plantarum NC8 | D-tagatose | - |
? | |
L-arabinose | - |
Lactiplantibacillus plantarum | L-ribulose | - |
? | |
L-arabinose | - |
Lactiplantibacillus plantarum NC8 | L-ribulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Lactiplantibacillus plantarum |
Synonyms | Comment | Organism |
---|---|---|
D-galactose isomerase | - |
Lactiplantibacillus plantarum |
L-AI NC8 | - |
Lactiplantibacillus plantarum |
L-arabinose aldose-ketose-isomerase | - |
Lactiplantibacillus plantarum |
L-arabinose isomerase | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Lactiplantibacillus plantarum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 65 | - |
Lactiplantibacillus plantarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable for 2 h in the presence of Mn2+ and Co2+ | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Lactiplantibacillus plantarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Lactiplantibacillus plantarum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | at 25°C for 50 min | Lactiplantibacillus plantarum |
5 | - |
for 24 h | Lactiplantibacillus plantarum |