Application | Comment | Organism |
---|---|---|
food industry | production of D-tagatose as a low-calorie sugar-substituting sweetener, the D-tagatose yield from the mutated enzyme is higher than from the wild type | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli of wild type and gali 153 mutant enzyme | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
M322V/S393T/V408A | error prone PCR mutagenesis using gali 152 as template, gali 153 with changes in 3 amino acids revealed a higher activity than gali 152 | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Geobacillus stearothermophilus | |
Dulcitol | weak inhibitor | Geobacillus stearothermophilus | |
erythritol | weak inhibitor | Geobacillus stearothermophilus | |
L-arabitol | strong inhibitory | Geobacillus stearothermophilus | |
ribitol | strong inhibitory | Geobacillus stearothermophilus | |
xylitol | weak inhibitor | Geobacillus stearothermophilus | |
Zn2+ | - |
Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
67 | - |
L-arabinose | wild type enzyme | Geobacillus stearothermophilus | |
100 | - |
L-arabinose | mutated enzyme | Geobacillus stearothermophilus | |
145 | - |
D-galactose | wild type enzyme | Geobacillus stearothermophilus | |
578 | - |
D-galactose | mutated enzyme | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activating | Geobacillus stearothermophilus | |
Ca2+ | activating | Geobacillus stearothermophilus | |
Co2+ | activating, highest activity of the mutated enzyme at 1.0 mM | Geobacillus stearothermophilus | |
Cu2+ | inhibitory | Geobacillus stearothermophilus | |
Fe2+ | activating | Geobacillus stearothermophilus | |
Mg2+ | activating | Geobacillus stearothermophilus | |
Mn2+ | activating, highest activity of the wild type enzyme at 1.0 mM | Geobacillus stearothermophilus | |
Mo2+ | activating | Geobacillus stearothermophilus | |
Zn2+ | inhibitory | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
SDS-PAGE | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q9S467 | - |
- |
Geobacillus stearothermophilus KCCM12265 | Q9S467 | - |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant wild type and mutant enzymes | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.3 | - |
of the recombinant wild type enzyme after purification | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | - |
Geobacillus stearothermophilus | D-tagatose | - |
? | |
D-galactose | - |
Geobacillus stearothermophilus KCCM12265 | D-tagatose | - |
? | |
L-arabinose | other aldoses like D-fucose, D-ribose, D-allose, D-mannose and D-xylose are poor substrates for the wild type and the mutated enzymes | Geobacillus stearothermophilus | L-ribulose | - |
? | |
L-arabinose | other aldoses like D-fucose, D-ribose, D-allose, D-mannose and D-xylose are poor substrates for the wild type and the mutated enzymes | Geobacillus stearothermophilus KCCM12265 | L-ribulose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-galactose isomerase | - |
Geobacillus stearothermophilus |
gali 152 | mutated L-arabinose isomerase from pL152 gene | Geobacillus stearothermophilus |
gali 153 | mutated L-arabinose isomerase from pL153 gene | Geobacillus stearothermophilus |
L-arabinose aldose-ketose-isomerase | - |
Geobacillus stearothermophilus |
L-arabinose isomerase | - |
Geobacillus stearothermophilus |
pL 151 | - |
Geobacillus stearothermophilus |
pL 152 | - |
Geobacillus stearothermophilus |
pL 153 | - |
Geobacillus stearothermophilus |
pL151 | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
wild type enzyme | Geobacillus stearothermophilus |
65 | - |
mutated enzyme gali 153 | Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 75 | - |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
wild type and mutated enzyme | Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Geobacillus stearothermophilus |