5.3.1.4: L-arabinose isomerase

This is an abbreviated version!
For detailed information about L-arabinose isomerase, go to the full flat file.

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Synonyms

AI, AraA, arabinose isomerase, D-galactose isomerase, ECAI, gali 152, gali 153, GSAI, GSAI 152, GSAI 153, Isomerase, L-arabinose, L-AI, L-AI NC8, L-AI US100, L-arabinose aldose-ketose-isomerase, L-arabinose isomerase, L-arabinose isomerase 1, L-arabinose isomerase 2, L-arabinose ketol-isomerase, pL 151, pL 152, pL 153, pL151, TNAI

ECTree

     5 Isomerases

         5.3 Intramolecular oxidoreductases

             5.3.1 Interconverting aldoses and ketoses, and related compounds

                5.3.1.4 L-arabinose isomerase

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Results	in table
4	Activating Compound
7544	AA Sequence
83	Application
1	CAS Registry Number
84	Cloned(Commentary)
9	Crystallization (Commentary)
2	Expression
3	General Stability
96	Inhibitors
73	kcat/KM [mM/s]
4	Ki Value [mM]
194	KM Value [mM]
157	Metals/Ions
87	Molecular Weight [Da]
83	Natural Substrates/ Products (Substrates)
180	Organism
5	Pathway
147	PDB ID
90	pH Optimum
25	pH Range
29	pH Stability
14	pI Value
155	Protein Variants
50	Purification (Commentary)
3	Reaction
45	Reaction Type
145	Reference
115	Specific Activity [micromol/min/mg]
3	Storage Stability
245	Substrates and Products (Substrate)
81	Subunits
156	Synonyms
1	Systematic Name
63	Temperature Optimum [°C]
23	Temperature Range [°C]
58	Temperature Stability [°C]
64	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.98 - 104.1

D-galactose

19 entries

0.06 - 0.82

D-tagatose

2 entries

241.7

L-aldose

Bacillus subtilis

C7F8M0

pH 7.5, 23°C, recombinant His6-tagged wild-type enzyme

701860

0.9 - 3657

L-arabinose

41 entries

241.7

L-ribulose

Bacillus subtilis

C7F8M0

pH 7.5, 23°C, recombinant His6-tagged wild-type enzyme

701860

0.98

D-galactose

Alicyclobacillus hesperidum

J9E728

mutant D478Q, pH 7.0, 65°C

746786

1.02

D-galactose

Escherichia coli

-

pH 7.0, 37°C, purified enzyme

690529

1.13

D-galactose

Alicyclobacillus hesperidum

J9E728

wild-type, pH 7.0, 65°C

746786

1.2

D-galactose

Halalkalibacterium halodurans

-

-

660806

1.26

D-galactose

Alicyclobacillus hesperidum

J9E728

mutant D478R, pH 7.0, 65°C

746786

1.36

D-galactose

Alicyclobacillus hesperidum

J9E728

mutant D478K, pH 7.0, 65°C

746786

1.58

D-galactose

Alicyclobacillus hesperidum

J9E728

mutant D478A, pH 7.0, 65°C

746786

1.65

D-galactose

Alicyclobacillus hesperidum

J9E728

mutant D478N, pH 7.0, 65°C

746786

2.2

D-galactose

Anoxybacillus flavithermus

F6KRI7

without borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C

714934

3.4

D-galactose

Geobacillus thermodenitrificans

Q6W9D4

-

662489

4.21

D-galactose

Escherichia coli

-

pH 7.0, 37°C, whole cells

690529

4.9

D-galactose

Thermoanaerobacterium saccharolyticum

K7SW59

in 25 mM Tris-HCl buffer (pH 7.5), at 60°C

726893

8.4

D-galactose

Geobacillus stearothermophilus

-

-

660806

17.4

D-galactose

Geobacillus thermodenitrificans

Q6W9D4

mutant C450S/N475K, pH 8, 55°C

746882

20.18

D-galactose

Geobacillus stearothermophilus

Q9S467

pH 8.0, 60°C, recombinant enzyme expressed in Escherichia coli

703788

28.4

D-galactose

[Clostridium] hylemonae

C0C0X3

pH 7.5, 35°C

749125

53.08

D-galactose

Geobacillus stearothermophilus

Q9S467

pH 8.0, 60°C, recombinant enzyme expressed in Bacillus subtilis

703788

78.5

D-galactose

Anoxybacillus flavithermus

F6KRI7

with 10 mM borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C

714934

104.1

D-galactose

Geobacillus thermodenitrificans

Q6W9D4

mutant F280N/C450S/N475K, pH 8, 55°C

746882

0.06

D-tagatose

Escherichia coli

-

pH 7.0, 37°C, purified enzyme

690529

0.82

D-tagatose

Escherichia coli

-

pH 7.0, 37°C, whole cells

690529

0.9

L-arabinose

Anoxybacillus flavithermus

F6KRI7

in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C

714934

11.2

L-arabinose

Geobacillus stearothermophilus

-

mutant enzyme E261L, at pH 7.5 and 70°C

727530

11.9

L-arabinose

Geobacillus stearothermophilus

-

mutant enzyme D118V, at pH 7.5 and 70°C

727530

12.8

L-arabinose

Geobacillus thermodenitrificans

Q6W9D4

mutant F280N/C450S/N475K, pH 8, 55°C

746882

12.9

L-arabinose

Pseudoalteromonas haloplanktis

Q3IG38

pH 7.0, 40°C

748640

14.2

L-arabinose

Geobacillus stearothermophilus

-

mutant enzyme D195V, at pH 7.5 and 70°C

727530

14.9

L-arabinose

Geobacillus stearothermophilus

-

mutant enzyme D333V, at pH 7.5 and 70°C

727530

15.88

L-arabinose

Halalkalibacterium halodurans

-

wild-type at pH 6 and 50°C

660770

19.62

L-arabinose

Alicyclobacillus acidocaldarius

Q2VMT2

mutant K269E at pH 6 and 65°C

660770

23.65

L-arabinose

Halalkalibacterium halodurans

-

wild-type at pH 7 and 50°C

660770

24.11

L-arabinose

Halalkalibacterium halodurans

-

mutant E268K at pH 6 and 50°C

660770

24.97

L-arabinose

Alicyclobacillus acidocaldarius

Q2VMT2

wild-type at pH 8 and 65°C

660770

25.8

L-arabinose

Alicyclobacillus acidocaldarius

Q2VMT2

mutant K269E at pH 8 and 65°C

660770

27.8

L-arabinose

Geobacillus stearothermophilus

-

mutant enzyme N460L, at pH 7.5 and 70°C

727530

28.55

L-arabinose

Alicyclobacillus acidocaldarius

Q2VMT2

wild-type at pH 7 and 65°C

660770

29.63

L-arabinose

Halalkalibacterium halodurans

-

mutant E268K at pH 8 and 50°C

660770

31

L-arabinose

Halalkalibacterium halodurans

-

-

660806

31.03

L-arabinose

Halalkalibacterium halodurans

-

wild-type at pH 8 and 50°C

660770

32.93

L-arabinose

Halalkalibacterium halodurans

-

mutant E268K at pH 7 and 50°C

660770

33.15

L-arabinose

Alicyclobacillus acidocaldarius

Q2VMT2

wild-type at pH 6 and 65°C

660770

33.8

L-arabinose

Geobacillus stearothermophilus

-

wild type enzyme, at pH 7.5 and 70°C

727530

34.1

L-arabinose

Geobacillus stearothermophilus

-

-

660806

37.2

L-arabinose

Alicyclobacillus acidocaldarius

Q2VMT2

mutant K269E at pH 7 and 65°C

660770

38.9

L-arabinose

Bacillus licheniformis

Q65J10

pH 7.5, 50°C, recombinant mutant Y333V

701823

50.3

L-arabinose

Bacillus licheniformis

Q65J10

pH 7.5, 50°C, recombinant mutant Y333I

701823

71.8

L-arabinose

Alicyclobacillus hesperidum

J9E728

wild-type, pH 7.0, 65°C

746786

72.9

L-arabinose

Alicyclobacillus hesperidum

J9E728

mutant D478R, pH 7.0, 65°C

746786

75.25

L-arabinose

Geobacillus stearothermophilus

Q9S467

pH 8.0, 60°C, recombinant enzyme expressed in Bacillus subtilis

703788

85

L-arabinose

Alicyclobacillus hesperidum

J9E728

mutant D478A, pH 7.0, 65°C

746786

99.6

L-arabinose

Alicyclobacillus hesperidum

J9E728

mutant D478K, pH 7.0, 65°C

746786

105

L-arabinose

Alicyclobacillus hesperidum

J9E728

mutant D478Q, pH 7.0, 65°C

746786

107.9

L-arabinose

Geobacillus stearothermophilus

Q9S467

pH 8.0, 60°C, recombinant enzyme expressed in Escherichia coli

703788

110

L-arabinose

Alicyclobacillus hesperidum

J9E728

mutant D478N, pH 7.0, 65°C

746786

113.6

L-arabinose

Geobacillus thermodenitrificans

Q6W9D4

-

662489

148.8

L-arabinose

Bacillus licheniformis

Q65J10

pH 7.5, 50°C, recombinant mutant Y333W

701823

188.2

L-arabinose

Bacillus licheniformis

Q65J10

pH 7.5, 50°C, recombinant mutant Y333F

701823

207

L-arabinose

Bacillus licheniformis

-

pH 7.5, 50°C

690589

207.5

L-arabinose

Bacillus licheniformis

Q65J10

pH 7.5, 50°C, recombinant wild-type enzyme

701823

207.6

L-arabinose

Bacillus licheniformis

Q65J10

pH and temperature not specified in the publication

714412

240.6

L-arabinose

Geobacillus thermodenitrificans

Q6W9D4

mutant C450S/N475K, pH 8, 55°C

746882

3657

L-arabinose

Weizmannia coagulans

-

at pH 7.0 and 70°C

728843