Literature summary for 5.3.1.4 extracted from

Zhan, Y.; Xu, Z.; Li, S.; Liu, X.; Xu, L.; Feng, X.; Xu, H.
Coexpression of ?-D-galactosidase and L-arabinose isomerase in the production of D-tagatose a functional sweetener (2014), J. Agric. Food Chem., 62, 2412-2417 .

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Application

Application	Comment	Organism
synthesis	coexpression of beta-D-galactosidase gene and L-arabinose isomerase mutant Q299K for synthesis of D-tagatose. Recombinant cells exhibit maximum D-tagatose producing activity at 34°C and pH 6.5 and in the presence of borate, 10 mM Fe2+, and 1 mM Mn2+. Cells can hydrolyze more than 95% lactose and convert 43% D-galactose into D-tagatose	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
Q299K	mutant displays similar optimum conditions as beta-galactosidase	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	mutant Q299K	Escherichia coli

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Release 2023.1 (January 2023)