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5.3.1.4: L-arabinose isomerase

This is an abbreviated version!
For detailed information about L-arabinose isomerase, go to the full flat file.

Word Map on EC 5.3.1.4

Reaction

beta-L-arabinopyranose
=
L-ribulose

Synonyms

AI, AraA, arabinose isomerase, D-galactose isomerase, ECAI, gali 152, gali 153, GSAI, GSAI 152, GSAI 153, Isomerase, L-arabinose, L-AI, L-AI NC8, L-AI US100, L-arabinose aldose-ketose-isomerase, L-arabinose isomerase, L-arabinose isomerase 1, L-arabinose isomerase 2, L-arabinose ketol-isomerase, pL 151, pL 152, pL 153, pL151, TNAI

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.1 Interconverting aldoses and ketoses, and related compounds
                5.3.1.4 L-arabinose isomerase

Purification

Purification on EC 5.3.1.4 - L-arabinose isomerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
40-80% ammoniumsulfate precipitation yielded 80% of the total activity
ammonium sulfate precipitation, epoxy-activated Sepharose column chromatography, and L-arabitol-agarose column chromatography
-
ammonium sulfate precipitation, Mono Q column chromatography, and Sephacryl S-200 gel filtration
-
DEAE Sepharose column chromatography and hydrophobic interaction chromatography
expression in Escherichia coli
-
Fractogel EMD DEAE chromatography and Fractogel EMD TMAE chromatography
Hiprep 16/10 Q-XL column chromatography, phenyl-Sepharose column chromatography, Mono Q HR 5/5 column chromatography, and Superdex 200 gel filtration
His-bind resin column chromatography, and Superdex 200 gel filtration
-
His-tag affinity column chromatography
-
MonoQ column chromatography
-
Ni-NTA affinity column chromatography
Ni-NTA agarose column chromatography and Superdex 200 gel filtration
-
Ni-NTA column chromatography
Ni-NTA resin column chromatography
Ni-Sepharose column chromatography, gel filtration
-
nickel-affinity column chromatography and Source 15Q column chromatography
of the recombinant enzyme by Ni-NTA-agarose resin chromatography
of the recombinant mutant proteins
of the recombinant mutant proteins by HisTrap HP chromatography
of the recombinant protein
of the recombinant wild type and mutant enzymes
recombinant enzyme 18fold from Bacillus subtilis
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment, ion exchange chromatography, and gel filtration
recombinant enzyme is purified to homogeneity by heat treatment, ion-exchange chromatography, and gel filtration
recombinant enzyme purified from Escherichia coli
recombinant enzyme, final purity about 97%
recombinant enzyme, to homogeneity
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography