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Literature summary for 5.3.1.4 extracted from
- Cloning, purification and biochemical characterization of metallic-ions independent and thermoactive l-arabinose isomerase from the Bacillus stearothermophilus US100 strain (2006), Biochim. Biophys. Acta, 1760, 191-199.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | production of D-tagatose | Geobacillus stearothermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Geobacillus stearothermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Geobacillus stearothermophilus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28.57 | - |
L-arabinose | - |
Geobacillus stearothermophilus | |
| 57 | - |
D-galactose | - |
Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | increase in activity at 80°C only, no effect at 65°C | Geobacillus stearothermophilus | |
| Mn2+ | increase in activity at 80°C only, no effect at 65°C | Geobacillus stearothermophilus | |
| additional information | activity and thermostability are totally independent of metallic ions up to 65°C, above 65°C, the enzyme's activity is also independent of metallic ions of its activity, but its thermostability is improved in the presence of only 0.2 mM Co2+ and 1 mM Mn2+ | Geobacillus stearothermophilus | |
| additional information | Ca2+, Ni2+, Zn2+, Mg2+, Fe2+, Cu2+ show no effect | Geobacillus stearothermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
calculated | Geobacillus stearothermophilus |
| 56000 | - |
monomer, SDS-PAGE | Geobacillus stearothermophilus |
| 56160 | - |
monomer, calculated from amino acid sequence | Geobacillus stearothermophilus |
| 225000 | - |
native gel electrophoresis | Geobacillus stearothermophilus |
| 225000 | - |
tetramer, SDS-PAGE | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | Geobacillus stearothermophilus | - |
D-tagatose | - |
? | |
| D-galactose | Geobacillus stearothermophilus US100 | - |
D-tagatose | - |
? | |
| L-arabinose | Geobacillus stearothermophilus | - |
L-ribulose | - |
? | |
| L-arabinose | Geobacillus stearothermophilus US100 | - |
L-ribulose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
| Geobacillus stearothermophilus | Q9S467 | - |
- |
| Geobacillus stearothermophilus US100 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant protein | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
with D-galactose as substrate | Geobacillus stearothermophilus |
| 41.3 | - |
with L-arabinose as substrate | Geobacillus stearothermophilus |
| 185 | - |
after purification with FPLC | Geobacillus stearothermophilus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C in 15% gylcerol | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | - |
Geobacillus stearothermophilus | D-tagatose | - |
? | |
| D-galactose | - |
Geobacillus stearothermophilus US100 | D-tagatose | - |
? | |
| L-arabinose | - |
Geobacillus stearothermophilus | L-ribulose | - |
? | |
| L-arabinose | - |
Geobacillus stearothermophilus US100 | L-ribulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Geobacillus stearothermophilus |
| tetramer | alpha4, 4 * 56000, SDS-PAGE, native gel electrophoresis | Geobacillus stearothermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-galactose isomerase | - |
Geobacillus stearothermophilus |
| L-AI US100 | - |
Geobacillus stearothermophilus |
| L-arabinose aldose-ketose-isomerase | - |
Geobacillus stearothermophilus |
| L-arabinose isomerase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
for conversion of D-galactose | Geobacillus stearothermophilus |
| 80 | - |
- |
Geobacillus stearothermophilus |
| 80 | - |
for conversion of L-arabinose | Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 90 | - |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermostability is totally independent of metallic ions up to 65°C, above 65°C, thermostability is improved in the presence of only 0.2 mM Co2+ and 1 mM Mn2+ | Geobacillus stearothermophilus |
| 50 | 65 | 100% activity in absence of metallic ions during 12 h | Geobacillus stearothermophilus |
| 65 | - |
until 65°C independent from metallic ions, above 65°C stability is improved in presence of 0.2 mM Co2+ and 1 mM Mn2+ | Geobacillus stearothermophilus |
| 70 | 80 | decrease in activity in absence of metallic ions | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Geobacillus stearothermophilus |
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