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0.057 - 71
DL-homocysteine
1.9
L-Gly(vinyl)
-
30°C, pH 8.0
1.7
L-homocysteine
pH 8.0, 37°C
2.1 - 40.4
L-methionine sulfone
2.5 - 6.7
L-methionine sulfoxide
1.9 - 44.4
L-Vinylglycine
2.1
O-acetyl-DL-homoserine
-
pH 8.0, 30°C
6.23 - 78
O-acetyl-L-homoserine
1.35 - 6.22
O-acetyl-L-serine
3.34 - 10.9
S-Benzyl-L-cysteine
0.57 - 8.05
S-ethyl-L-cysteine
2.07 - 7.05
S-ethyl-L-homocysteine
0.12 - 46.3
S-methyl-L-cysteine
0.24 - 17.46
trifluoromethionine
additional information
L-methionine
0.41
alliin
mutant C115A, pH 8.0, 30°C
11.31
alliin
mutant C115H, pH 8.0, 30°C
0.057 - 0.65
DL-homocysteine
wild-type ehMGL2, pH 7.0, 37°C
0.88
DL-homocysteine
mutant Y58F, pH 8.0, 30°C
1.36
DL-homocysteine
pH 8.0, 37°C, recombinant mutant Y114F/C116H
1.74
DL-homocysteine
mutant enzyme C116A, at 37°C
2.59
DL-homocysteine
mutant enzyme C116S, at 37°C
3.13
DL-homocysteine
wild-type ehMGL1, pH 7.0, 37°C
3.91
DL-homocysteine
wild-type ehMGL1, pH 7.0, 37°C
5.03
DL-homocysteine
pH 8.0, 37°C, recombinant mutant K240N
5.1
DL-homocysteine
wild-type
8.5
DL-homocysteine
-
pH 8.0, 30°C
8.5
DL-homocysteine
-
30°C, pH 8.0
8.51
DL-homocysteine
recombinant enzyme
10.56
DL-homocysteine
wild-type ehMGL2, pH 7.0, 37°C
22.7
DL-homocysteine
pH 8.0, 37°C, recombinant mutant K240R
28.8
DL-homocysteine
pH 8.0, 37°C, recombinant mutant K240M
30
DL-homocysteine
mutant enzyme C116H, at 37°C
44.84
DL-homocysteine
wild type enzyme, at 37°C
48.4
DL-homocysteine
pH 8.0, 37°C, recombinant mutant C166H
62.2
DL-homocysteine
pH 8.0, 37°C, recombinant mutant K240I
71
DL-homocysteine
pH 8.0, 37°C, recombinant wild-type enzyme
0.5
DL-homoserine
-
30°C, pH 8.0
0.52
DL-homoserine
-
pH 8.0, 30°C
1.25
ethiin
mutant C115A, pH 8.0, 30°C
5.59
ethiin
mutant C115H, pH 8.0, 30°C
0.03
L-cysteine
pH 8.0, 37°C
0.43
L-cysteine
mutant enzyme C116A, at 37°C
0.57
L-cysteine
mutant enzyme C116S, at 37°C
0.67
L-cysteine
mutant Y108F, pH 7.0, 37°C
0.69
L-cysteine
pH 7.6, 37°C
0.78
L-cysteine
mutant C110S, pH 7.0, 37°C
0.79
L-cysteine
pH 7.6, 37°C
0.8
L-cysteine
wild-type ehMGL2, pH 7.0, 37°C
0.89
L-cysteine
pH 8.0, 37°C, recombinant mutant Y114F/C116H
1.01
L-cysteine
mutant C110G, pH 7.0, 37°C
1.06
L-cysteine
pH 8.0, 37°C, recombinant mutant K240N
1.45
L-cysteine
pH 8.0, 37°C, recombinant mutant K240R
1.59
L-cysteine
wild-type ehMGL1, pH 7.0, 37°C
1.66
L-cysteine
pH 8.0, 37°C, recombinant mutant K240M
1.86
L-cysteine
pH 7.6, 37°C
2.13
L-cysteine
pH 8.0, 37°C, recombinant wild-type enzyme
2.23
L-cysteine
pH 8.0, 37°C, recombinant mutant K240I
2.24
L-cysteine
wild-type
2.27
L-cysteine
wild type enzyme, at 37°C
2.3
L-cysteine
-
30°C, pH 8.0
2.33
L-cysteine
recombinant enzyme
23.3
L-cysteine
pH 8.0, 37°C, recombinant mutant C166H
43.8
L-cysteine
mutant enzyme C116H, at 37°C
0.017
L-ethionine
-
37°C, pH 8.0
6.2
L-ethionine
wild-type
6.78
L-ethionine
recombinant enzyme
33.4
L-ethionine
-
37°C, pH 8.0
0.03
L-methionine
pH 8.0, 37°C, recombinant mutant C166H
0.07
L-methionine
mutant enzyme C116H, at 37°C
0.1
L-methionine
pH 8.0, 23°C
0.17
L-methionine
-
37°C, pH 8.0
0.22
L-methionine
mutant C115A, pH 8.0, 30°C
0.36
L-methionine
mutant C110G, pH 7.0, 37°C
0.47
L-methionine
mutant C113S, pH 7.0, 37°C
0.93
L-methionine
mutant C110S, pH 7.0, 37°C
1.11
L-methionine
wild-type ehMGL2, pH 7.0, 37°C
1.46
L-methionine
mutant enzyme C116A, at 37°C
1.77
L-methionine
mutant enzyme C116S, at 37°C
1.82
L-methionine
wild-type ehMGL1, pH 7.0, 37°C
2.66
L-methionine
mutant Y58F, pH 8.0, 30°C
2.9
L-methionine
pH 8.0, 37°C
3.9
L-methionine
-
pH 8.0, 30°C, recombinant enzyme
5.47
L-methionine
pH 7.5, 37°C
6.2
L-methionine
recombinant enzyme
6.2
L-methionine
-
pH 8.0, 30°C
6.2
L-methionine
-
30°C, pH 8.0
6.5
L-methionine
wild-type
7.4
L-methionine
mutant P360Q, pH 7.2, 30°C
7.9
L-methionine
wild-type, pH 7.2, 30°C
8
L-methionine
mutant A366Y, pH 7.2, 30°C
9
L-methionine
mutant P357I, pH 7.2, 30°C
9.86
L-methionine
-
pH 8.0, 30°C, recombinant enzyme
12
L-methionine
-
pH 8.0, 30°C, recombinant enzyme
15.1
L-methionine
mutant V358Y, pH 7.2, 30°C
25.39
L-methionine
wild type enzyme, at 37°C
33.4
L-methionine
pH 8.0, 37°C, recombinant wild-type enzyme
48.6
L-methionine
-
37°C, pH 8.0
2.1
L-methionine sulfone
-
pH 8.0, 30°C
34.13
L-methionine sulfone
pH 7.5, 37°C
40.4
L-methionine sulfone
-
37°C, pH 8.0
2.5
L-methionine sulfoxide
-
pH 8.0, 30°C
2.7
L-methionine sulfoxide
-
pH 8.0, 30°C, recombinant enzyme
5.05
L-methionine sulfoxide
-
pH 8.0, 30°C, recombinant enzyme
6.7
L-methionine sulfoxide
-
pH 8.0, 30°C, recombinant enzyme
1.9
L-Vinylglycine
-
pH 8.0, 30°C
44.4
L-Vinylglycine
-
37°C, pH 8.0
1.63
methiin
mutant C115A, pH 8.0, 30°C
5.14
methiin
mutant C115H, pH 8.0, 30°C
6.23
O-acetyl-L-homoserine
mutant C115A, pH 8.0, 30°C
18.29
O-acetyl-L-homoserine
mutant C115H, pH 8.0, 30°C
78
O-acetyl-L-homoserine
-
37°C, pH 8.0
1.35
O-acetyl-L-serine
mutant C115A, pH 8.0, 30°C
1.74
O-acetyl-L-serine
wild-type ehMGL1, pH 7.0, 37°C
2.93
O-acetyl-L-serine
mutant C115H, pH 8.0, 30°C
6.22
O-acetyl-L-serine
wild-type ehMGL2, pH 7.0, 37°C
3.34
S-Benzyl-L-cysteine
mutant Y58F, pH 8.0, 30°C
5.8
S-Benzyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
8.16
S-Benzyl-L-cysteine
recombinant enzyme
8.2
S-Benzyl-L-cysteine
-
30°C, pH 8.0
8.5
S-Benzyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
10
S-Benzyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
10.9
S-Benzyl-L-cysteine
wild-type
0.57
S-ethyl-L-cysteine
mutant C115A, pH 8.0, 30°C
2.38
S-ethyl-L-cysteine
mutant Y58F, pH 8.0, 30°C
5
S-ethyl-L-cysteine
-
30°C, pH 8.0
5.03
S-ethyl-L-cysteine
recombinant enzyme
5.79
S-ethyl-L-cysteine
-
37°C, pH 8.0
6.3
S-ethyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
6.5
S-ethyl-L-cysteine
mutant C115H, pH 8.0, 30°C
6.8
S-ethyl-L-cysteine
wild-type
7.08
S-ethyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
8.05
S-ethyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
2.07
S-ethyl-L-homocysteine
mutant Y58F, pH 8.0, 30°C
3.84
S-ethyl-L-homocysteine
-
pH 8.0, 30°C, recombinant enzyme
5.89
S-ethyl-L-homocysteine
-
pH 8.0, 30°C, recombinant enzyme
6.8
S-ethyl-L-homocysteine
-
30°C, pH 8.0
7.05
S-ethyl-L-homocysteine
-
pH 8.0, 30°C, recombinant enzyme
0.12
S-methyl-L-cysteine
pH 8.0, 37°C, recombinant mutant Y114F/C116H
1.38
S-methyl-L-cysteine
mutant C115A, pH 8.0, 30°C
1.58
S-methyl-L-cysteine
pH 8.0, 37°C, recombinant wild-type enzyme
4.6
S-methyl-L-cysteine
recombinant enzyme
4.6
S-methyl-L-cysteine
-
30°C, pH 8.0
5.53
S-methyl-L-cysteine
-
37°C, pH 8.0
5.9
S-methyl-L-cysteine
-
-
5.97
S-methyl-L-cysteine
wild-type
23.08
S-methyl-L-cysteine
mutant C115H, pH 8.0, 30°C
46.3
S-methyl-L-cysteine
pH 8.0, 37°C, recombinant mutant C166H
0.24
trifluoromethionine
mutant C113S, pH 7.0, 37°C
0.81
trifluoromethionine
-
-
0.81
trifluoromethionine
wild-type ehMGL1, pH 7.0, 37°C
0.97
trifluoromethionine
wild-type ehMGL2, pH 7.0, 37°C
1.19
trifluoromethionine
mutant R58A, pH 7.0, 37°C
1.26
trifluoromethionine
mutant R55A, pH 7.0, 37°C
2.22
trifluoromethionine
mutant Y108F, pH 7.0, 37°C
5.8
trifluoromethionine
mutant Y111F, pH 7.0, 37°C
17.46
trifluoromethionine
wild-type ehMGL2, pH 7.0, 37°C
additional information
L-methionine
-
values between 25.3 to 48.6, depending on publications
additional information
S-ethyl-L-cysteine
-
37°C, pH 8.0
additional information
S-methyl-L-cysteine
-
37°C, pH 8.0
4.4.1.11 methionine gamma-lyase
results (
results (
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