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Literature summary for 4.4.1.11 extracted from

  • Suwabe, K.; Yoshida, Y.; Nagano, K.; Yoshimura, F.
    Identification of an L-methionine gamma-lyase involved in the production of hydrogen sulfide from L-cysteine in Fusobacterium nucleatum subsp. nucleatum ATCC 25586 (2011), Microbiology, 157, 2992-3000.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Fusobacterium nucleatum subsp. nucleatum
-
Porphyromonas gingivalis
-
Treponema denticola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
L-cysteine pH 7.6, 37°C Treponema denticola
0.32
-
L-cysteine pH 7.6, 37°C Fusobacterium nucleatum subsp. nucleatum
0.63
-
L-cysteine pH 7.6, 37°C Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, SDS-PAGE, 2 * 43300, calculated Porphyromonas gingivalis
43000
-
4 * 43000, SDS-PAGE, 4 * 43300, calculated Fusobacterium nucleatum subsp. nucleatum
43300
-
2 * 43000, SDS-PAGE, 2 * 43300, calculated Porphyromonas gingivalis
43300
-
4 * 43000, SDS-PAGE, 4 * 43300, calculated Fusobacterium nucleatum subsp. nucleatum
43500
-
4 * 43500, calculated and SDS-PAGE Treponema denticola
81400
-
gel filtration Porphyromonas gingivalis
154100
-
gel filtration Fusobacterium nucleatum subsp. nucleatum
167500
-
gel filtration Treponema denticola

Organism

Organism UniProt Comment Textmining
Fusobacterium nucleatum subsp. nucleatum Q8RDT4
-
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 Q8RDT4
-
-
Porphyromonas gingivalis Q7MX71
-
-
Porphyromonas gingivalis W83 Q7MX71
-
-
Treponema denticola Q73KL7
-
-
Treponema denticola ATCC 35405 Q73KL7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + H2O
-
Fusobacterium nucleatum subsp. nucleatum sulfide + NH3 + pyruvate
-
?
L-cysteine + H2O
-
Porphyromonas gingivalis sulfide + NH3 + pyruvate
-
?
L-cysteine + H2O
-
Treponema denticola sulfide + NH3 + pyruvate
-
?
L-cysteine + H2O
-
Porphyromonas gingivalis W83 sulfide + NH3 + pyruvate
-
?
L-cysteine + H2O
-
Treponema denticola ATCC 35405 sulfide + NH3 + pyruvate
-
?
L-cysteine + H2O
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 sulfide + NH3 + pyruvate
-
?
L-methionine + H2O
-
Fusobacterium nucleatum subsp. nucleatum methanethiol + NH3 + 2-oxobutanoate
-
?
L-methionine + H2O
-
Porphyromonas gingivalis methanethiol + NH3 + 2-oxobutanoate
-
?
L-methionine + H2O
-
Treponema denticola methanethiol + NH3 + 2-oxobutanoate
-
?
L-methionine + H2O
-
Porphyromonas gingivalis W83 methanethiol + NH3 + 2-oxobutanoate
-
?
L-methionine + H2O
-
Treponema denticola ATCC 35405 methanethiol + NH3 + 2-oxobutanoate
-
?
L-methionine + H2O
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 methanethiol + NH3 + 2-oxobutanoate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE, 2 * 43300, calculated Porphyromonas gingivalis
tetramer 4 * 43000, SDS-PAGE, 4 * 43300, calculated Fusobacterium nucleatum subsp. nucleatum
tetramer 4 * 43500, calculated and SDS-PAGE Treponema denticola

Synonyms

Synonyms Comment Organism
Fn1419
-
Fusobacterium nucleatum subsp. nucleatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.69
-
L-cysteine pH 7.6, 37°C Fusobacterium nucleatum subsp. nucleatum
0.79
-
L-cysteine pH 7.6, 37°C Treponema denticola
1.86
-
L-cysteine pH 7.6, 37°C Porphyromonas gingivalis

General Information

General Information Comment Organism
physiological function the amount of H2S produced by Fn1419 is estimated to be 1.9% of the total H2S produced from L-cysteine in Fusobacterium nucleatum ATCC 25586 Fusobacterium nucleatum subsp. nucleatum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.15
-
L-cysteine pH 7.6, 37°C Fusobacterium nucleatum subsp. nucleatum
2.96
-
L-cysteine pH 7.6, 37°C Porphyromonas gingivalis
4.46
-
L-cysteine pH 7.6, 37°C Treponema denticola