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Literature summary for 4.4.1.11 extracted from

  • Sato, D.; Yamagata, W.; Harada, S.; Nozaki, T.
    Kinetic characterization of methionine gamma-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis (2008), FEBS J., 275, 548-560.
    View publication on PubMed
Search for more literature for 4.4.1.11

Protein Variants

Protein Variants Comment Organism
C110G decrease in kcat values for both L-methionine and L-cysteine Entamoeba histolytica
C110S slightly reduced catalytic activites Entamoeba histolytica
C113G 20% increase in activity with L-methionine Entamoeba histolytica
C113S reduced catalytic activites Entamoeba histolytica
R55A decrease in catalytic activity Entamoeba histolytica
R58A complete loss of activity Entamoeba histolytica
Y108F almost 100% reduction in alpha-gamma-elimination of both L-methionine and homocysteine Entamoeba histolytica
Y111F about 80% reduction in alpha-gamma-elimination of both L-methionine and homocysteine Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 trifluoromethionine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
0.19
-
 L-methionine mutant C110G, pH 7.0, 37°C Entamoeba histolytica
0.29
-
 trifluoromethionine mutant Y111F, pH 7.0, 37°C Entamoeba histolytica
0.34
-
 L-cysteine mutant C110G, pH 7.0, 37°C Entamoeba histolytica
0.46
-
 L-cysteine mutant C110S, pH 7.0, 37°C Entamoeba histolytica
0.57
-
 trifluoromethionine mutant Y108F, pH 7.0, 37°C Entamoeba histolytica
0.61
-
 L-methionine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
0.64
-
 L-cysteine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
0.72
-
 L-methionine mutant C110S, pH 7.0, 37°C Entamoeba histolytica
0.83
-
 trifluoromethionine mutant R55A, pH 7.0, 37°C Entamoeba histolytica
0.92
-
 trifluoromethionine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
1.01
-
 L-cysteine mutant Y108F, pH 7.0, 37°C Entamoeba histolytica
1.47
-
 DL-homocysteine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
1.62
-
 trifluoromethionine mutant R58A, pH 7.0, 37°C Entamoeba histolytica
1.7
-
 L-cysteine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
3.03
-
 DL-homocysteine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
3.58
-
 L-methionine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
5.45
-
 trifluoromethionine mutant C113S, pH 7.0, 37°C Entamoeba histolytica
6.28
-
 O-acetyl-L-serine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
15.12
-
 L-methionine mutant C113S, pH 7.0, 37°C Entamoeba histolytica
52.33
-
 O-acetyl-L-serine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica Q86D27 isoform ehMGL2
-
Entamoeba histolytica Q86D28 isoform ehMGL1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-homocysteine + H2O
-
 Entamoeba histolytica hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
L-cysteine + H2O
-
 Entamoeba histolytica sulfide + NH3 + pyruvate
-
 ?
L-methionine + H2O
-
 Entamoeba histolytica methanethiol + NH3 + 2-oxobutanoate
-
 ?
additional information under normal conditions, isoform ehMGL1 is involved in degradation of L-methionine Entamoeba histolytica ?
-
 ?
additional information under normal conditions, isoform ehMGL2 is not involved degradation of L-methionine Entamoeba histolytica ?
-
 ?
O-acetyl-L-serine + H2O
-
 Entamoeba histolytica 2-oxopropanoate + NH3 + acetate
-
 ?
trifluoromethionine + H2O
-
 Entamoeba histolytica trifluoromethanethiol + NH3 + 2-oxobutanoate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.057 0.65 DL-homocysteine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
0.24
-
 trifluoromethionine mutant C113S, pH 7.0, 37°C Entamoeba histolytica
0.36
-
 L-methionine mutant C110G, pH 7.0, 37°C Entamoeba histolytica
0.47
-
 L-methionine mutant C113S, pH 7.0, 37°C Entamoeba histolytica
0.67
-
 L-cysteine mutant Y108F, pH 7.0, 37°C Entamoeba histolytica
0.78
-
 L-cysteine mutant C110S, pH 7.0, 37°C Entamoeba histolytica
0.8
-
 L-cysteine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
0.81
-
 trifluoromethionine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
0.93
-
 L-methionine mutant C110S, pH 7.0, 37°C Entamoeba histolytica
0.97
-
 trifluoromethionine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
1.01
-
 L-cysteine mutant C110G, pH 7.0, 37°C Entamoeba histolytica
1.11
-
 L-methionine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
1.19
-
 trifluoromethionine mutant R58A, pH 7.0, 37°C Entamoeba histolytica
1.26
-
 trifluoromethionine mutant R55A, pH 7.0, 37°C Entamoeba histolytica
1.59
-
 L-cysteine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
1.74
-
 O-acetyl-L-serine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
1.82
-
 L-methionine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
2.22
-
 trifluoromethionine mutant Y108F, pH 7.0, 37°C Entamoeba histolytica
3.13
-
 DL-homocysteine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
3.91
-
 DL-homocysteine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
5.8
-
 trifluoromethionine mutant Y111F, pH 7.0, 37°C Entamoeba histolytica
6.22
-
 O-acetyl-L-serine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
10.56
-
 DL-homocysteine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
17.46
-
 trifluoromethionine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica