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Literature summary for 4.4.1.11 extracted from

  • Morozova, E.A.; Kulikova, V.V.; Yashin, D.V.; Anufrieva, N.V.; Anisimova, N.Y.; Revtovich, S.V.; Kotlov, M.I.; Belyi, Y.F.; Pokrovsky, V.S.; Demidkina, T.V.
    Kinetic parameters and cytotoxic activity of recombinant methionine gamma-lyase from Clostridium tetani, Clostridium sporogenes, Porphyromonas gingivalis and Citrobacter freundii (2013), Acta Naturae, 5, 92-98.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.11

Application

Application Comment Organism
medicine the enzyme is of interest as an anticancer agent, since the growth of malignant cells of various origins (unlike the growth of normal cells) is accompanied by obligatory methionine utilization, and the enzyme is absent in mammalian cells. IC50 of MGL for several tumor cell cultures, overview Citrobacter freundii
medicine the enzyme is of interest as an anticancer agent, since the growth of malignant cells of various origins (unlike the growth of normal cells) is accompanied by obligatory methionine utilization, and the enzyme is absent in mammalian cells. IC50 of MGL for several tumor cell cultures, overview Clostridium sporogenes
medicine the enzyme is of interest as an anticancer agent, since the growth of malignant cells of various origins (unlike the growth of normal cells) is accompanied by obligatory methionine utilization, and the enzyme is absent in mammalian cells. IC50 of MGL for several tumor cell cultures, overview Clostridium tetani

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Clostridium sporogenes
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Clostridium tetani
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Porphyromonas gingivalis
recombinant enzyme expression in Escherichia coli strain BL21 Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics for beta- and gamma-elimination reactions, overview Citrobacter freundii
additional information
-
 additional information steady-state kinetics for beta- and gamma-elimination reactions, overview Clostridium sporogenes
additional information
-
 additional information steady-state kinetics for beta- and gamma-elimination reactions, overview Clostridium tetani
additional information
-
 additional information steady-state kinetics for beta- and gamma-elimination reactions, overview Porphyromonas gingivalis
0.278
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
0.348
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
0.358
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
0.432
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
0.545
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
0.72
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
0.766
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
0.93
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
0.947
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
1.47
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
1.77
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
2.17
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
7.07
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Clostridium tetani
12.22
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
33.51
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44040
-
 4 * 44040, His-tagged enzyme, sequence calcualtion Clostridium tetani
44080
-
 4 * 44080, His-tagged enzyme, sequence calcualtion Porphyromonas gingivalis
44360
-
 4 * 44360, His-tagged enzyme, sequence calcualtion Clostridium sporogenes
170000
-
 about, recombinant detagged enzyme, gel filtration Clostridium sporogenes
170000
-
 about, recombinant detagged enzyme, gel filtration Clostridium tetani
170000
-
 about, recombinant detagged enzyme, gel filtration Porphyromonas gingivalis
258000
-
 native enzyme, gel filtration Clostridium tetani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine + H2O Citrobacter freundii gamma-elimination reaction methanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine + H2O Clostridium sporogenes gamma-elimination reaction methanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine + H2O Clostridium tetani gamma-elimination reaction methanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine + H2O Porphyromonas gingivalis gamma-elimination reaction methanethiol + NH3 + 2-oxobutanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
-
-
Clostridium sporogenes
-
-
-
Clostridium tetani
-
-
-
Porphyromonas gingivalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21 Citrobacter freundii
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin and elimination by gel filtration Clostridium sporogenes
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin and elimination by gel filtration Clostridium tetani
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin and elimination by gel filtration Porphyromonas gingivalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5
-
 purified His-tagged recombinant enzyme, pH 8.0, 30°C, substrate L-methionine Porphyromonas gingivalis
10.2
-
 purified recombinant enzyme, pH 8.0, 30°C, substrate L-methionine Citrobacter freundii
12.8
-
 purified His-tagged recombinant enzyme, pH 8.0, 30°C, substrate L-methionine Clostridium sporogenes
16.6
-
 purified His-tagged recombinant enzyme, pH 8.0, 30°C, substrate L-methionine Clostridium tetani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine + H2O gamma-elimination reaction Citrobacter freundii methanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine + H2O gamma-elimination reaction Clostridium sporogenes methanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine + H2O gamma-elimination reaction Clostridium tetani methanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine + H2O gamma-elimination reaction Porphyromonas gingivalis methanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine sulfoxide + H2O gamma-elimination reaction Citrobacter freundii ?
-
 ?
L-methionine sulfoxide + H2O gamma-elimination reaction Clostridium sporogenes ?
-
 ?
L-methionine sulfoxide + H2O gamma-elimination reaction Clostridium tetani ?
-
 ?
L-methionine sulfoxide + H2O gamma-elimination reaction Porphyromonas gingivalis ?
-
 ?
additional information substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia Citrobacter freundii ?
-
 ?
additional information substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia Clostridium sporogenes ?
-
 ?
additional information substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia Clostridium tetani ?
-
 ?
additional information substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia Porphyromonas gingivalis ?
-
 ?
S-benzyl-L-cysteine + H2O beta-elimination reaction Citrobacter freundii ?
-
 ?
S-benzyl-L-cysteine + H2O beta-elimination reaction Clostridium sporogenes ?
-
 ?
S-benzyl-L-cysteine + H2O beta-elimination reaction Clostridium tetani ?
-
 ?
S-benzyl-L-cysteine + H2O beta-elimination reaction Porphyromonas gingivalis ?
-
 ?
S-ethyl-L-cysteine + H2O beta-elimination reaction Citrobacter freundii ?
-
 ?
S-ethyl-L-cysteine + H2O beta-elimination reaction Clostridium sporogenes ?
-
 ?
S-ethyl-L-cysteine + H2O beta-elimination reaction Clostridium tetani ?
-
 ?
S-ethyl-L-cysteine + H2O beta-elimination reaction Porphyromonas gingivalis ?
-
 ?
S-ethyl-L-homocysteine + H2O gamma-elimination reaction Citrobacter freundii ?
-
 ?
S-ethyl-L-homocysteine + H2O gamma-elimination reaction Clostridium sporogenes ?
-
 ?
S-ethyl-L-homocysteine + H2O gamma-elimination reaction Clostridium tetani ?
-
 ?
S-ethyl-L-homocysteine + H2O gamma-elimination reaction Porphyromonas gingivalis ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 44040, His-tagged enzyme, sequence calcualtion Clostridium tetani
tetramer 4 * 44080, His-tagged enzyme, sequence calcualtion Porphyromonas gingivalis
tetramer 4 * 44360, His-tagged enzyme, sequence calcualtion Clostridium sporogenes
tetramer dimer of dimers, 2 active sites, structure model Citrobacter freundii

Synonyms

Synonyms Comment Organism
MGL
-
 Citrobacter freundii
MGL
-
 Clostridium sporogenes
MGL
-
 Clostridium tetani
MGL
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Citrobacter freundii
30
-
 assay at Clostridium sporogenes
30
-
 assay at Clostridium tetani
30
-
 assay at Porphyromonas gingivalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Clostridium tetani
3.84
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
3.9
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
5.05
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
5.8
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
5.89
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
6.3
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
6.7
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
7.05
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
7.08
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
8.05
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
8.5
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
9.86
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
10
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
12
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Clostridium tetani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Citrobacter freundii
8
-
 assay at Clostridium sporogenes
8
-
 assay at Clostridium tetani
8
-
 assay at Porphyromonas gingivalis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Citrobacter freundii
pyridoxal 5'-phosphate
-
 Clostridium sporogenes
pyridoxal 5'-phosphate
-
 Clostridium tetani
pyridoxal 5'-phosphate
-
 Porphyromonas gingivalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
0.382
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Clostridium tetani
0.413
-
 L-methionine sulfoxide pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
2.2
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
3.71
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
3.94
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
4.13
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Porphyromonas gingivalis
9.83
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
10.8
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
11.1
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
12.7
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Clostridium tetani
17.6
-
 S-ethyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
22.8
-
 L-methionine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
25.4
-
 S-ethyl-L-homocysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes
28.7
-
 S-Benzyl-L-cysteine pH 8.0, 30°C, recombinant enzyme Clostridium sporogenes