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Literature summary for 4.4.1.11 extracted from

  • Inoue, H.; Inagaki, K.; Adachi, N.; Tamura, T.; Esaki, N.; Soda, K.; Tanaka, H.
    Role of tyrosine 114 of L-methionine gamma-lyase from Pseudomonas putida (2000), Biosci. Biotechnol. Biochem., 64, 2336-2343.
    View publication on PubMed
Search for more literature for 4.4.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
Y114F 0.1% of wild-type kcat for L-methionine Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 L-ethionine 37°C, pH 8.0 Pseudomonas putida
0.4
-
 S-methyl-L-cysteine 37°C, pH 8.0 Pseudomonas putida
0.48
-
 S-ethyl-L-cysteine 37°C, pH 8.0 Pseudomonas putida
0.9
-
 L-methionine 37°C, pH 8.0 Pseudomonas putida
2.22
-
 O-acetyl-L-homoserine 37°C, pH 8.0 Pseudomonas putida
7.22
-
 L-Vinylglycine 37°C, pH 8.0 Pseudomonas putida
8.22
-
 L-methionine sulfone 37°C, pH 8.0 Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine + H2O Pseudomonas putida
-
 methanethiol + NH3 + 2-oxobutanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant L-methionine gamma-lyase Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ethionine + H2O
-
 Pseudomonas putida ethanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine + H2O
-
 Pseudomonas putida methanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine sulfone
-
 Pseudomonas putida ?
-
 ?
L-vinylglycine
-
 Pseudomonas putida ?
-
 ?
O-acetyl-L-homoserine
-
 Pseudomonas putida ?
-
 ?
S-ethyl-L-cysteine
-
 Pseudomonas putida ?
-
 ?
S-methyl-L-cysteine
-
 Pseudomonas putida ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 S-methyl-L-cysteine 37°C, pH 8.0 Pseudomonas putida
additional information
-
 S-ethyl-L-cysteine 37°C, pH 8.0 Pseudomonas putida
0.017
-
 L-ethionine 37°C, pH 8.0 Pseudomonas putida
0.17
-
 L-methionine 37°C, pH 8.0 Pseudomonas putida
5.53
-
 S-methyl-L-cysteine 37°C, pH 8.0 Pseudomonas putida
5.79
-
 S-ethyl-L-cysteine 37°C, pH 8.0 Pseudomonas putida
33.4
-
 L-ethionine 37°C, pH 8.0 Pseudomonas putida
40.4
-
 L-methionine sulfone 37°C, pH 8.0 Pseudomonas putida
44.4
-
 L-Vinylglycine 37°C, pH 8.0 Pseudomonas putida
48.6
-
 L-methionine 37°C, pH 8.0 Pseudomonas putida
78
-
 O-acetyl-L-homoserine 37°C, pH 8.0 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pseudomonas putida