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4.2.3.124: 2-deoxy-scyllo-inosose synthase

This is an abbreviated version!
For detailed information about 2-deoxy-scyllo-inosose synthase, go to the full flat file.

Word Map on EC 4.2.3.124

Reaction

D-glucose 6-phosphate
=
2-deoxy-L-scyllo-inosose
 +
phosphate

Synonyms

AlloH, btrC, BtrC2, DOI synthase, DOIS, KanA, kanC, neoC, tbmA

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.124 2-deoxy-scyllo-inosose synthase

Purification

Purification on EC 4.2.3.124 - 2-deoxy-scyllo-inosose synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 148fold by ammonium sulfate fractionation, anion exchange and affinity chromatography, and gel filtration, followed by another different step of anion exchange chromatography
native enzyme by ammonium sulfate fractionation, dialysis and two different steps of anion exchange chromatography
recombinant AlloH from Escherichia coli by anion exchange chromatography and gel filtration
-
recombinant BtrC 6.0fold from Escherichia coli strain BL21(DE3)
recombinant BtrC from Escherichia coli by gel filtration and hydrophobic interaction chromatography
recombinant BtrC2 from Escherichia coli strain JM109 by ammonium sulfate fractionation, anion exchange chromatography, ultrafiltration, and gel filtration
recombinant enzyme by gel filtration, ultracentrifugation, and hydrophobic interaction chromatography