Cloned (Comment) | Organism |
---|---|
gene btrC, expression in Escherichia coli | Niallia circulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-carbaglucose 6-phosphate | a mechanism-based irreversible inhibitor, synthesis, overview. The alpha,beta-unsaturated intermediate traps a specific nucleophilic group in the active site through the Michael-type 1,4-addition. The covalently modified amino acid residue is Lys141 | Niallia circulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required | Niallia circulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40608 | - |
x * 40608, mass spectrometry, x * 40768, DL-carbaglucose 6-phosphate-enzyme complex, mass spectrometry | Niallia circulans |
40768 | - |
x * 40608, mass spectrometry, x * 40768, DL-carbaglucose 6-phosphate-enzyme complex, mass spectrometry | Niallia circulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | Niallia circulans | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | Niallia circulans SANK72073 | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | Q9S5E2 | gene btrC | - |
Niallia circulans SANK72073 | Q9S5E2 | gene btrC | - |
Purification (Comment) | Organism |
---|---|
recombinant BtrC from Escherichia coli by gel filtration and hydrophobic interaction chromatography | Niallia circulans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate | reaction mechanism, overview | Niallia circulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | - |
Niallia circulans | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | - |
Niallia circulans SANK72073 | 2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40608, mass spectrometry, x * 40768, DL-carbaglucose 6-phosphate-enzyme complex, mass spectrometry | Niallia circulans |
Synonyms | Comment | Organism |
---|---|---|
btrC | - |
Niallia circulans |
DOIS | - |
Niallia circulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
assay at | Niallia circulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Niallia circulans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Niallia circulans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.224 | - |
DL-carbaglucose 6-phosphate | pH 7.7, 46°C | Niallia circulans |