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4.2.3.124: 2-deoxy-scyllo-inosose synthase

This is an abbreviated version!
For detailed information about 2-deoxy-scyllo-inosose synthase, go to the full flat file.

Word Map on EC 4.2.3.124

Reaction

D-glucose 6-phosphate
=
2-deoxy-L-scyllo-inosose
 +
phosphate

Synonyms

AlloH, btrC, BtrC2, DOI synthase, DOIS, KanA, kanC, neoC, tbmA

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.124 2-deoxy-scyllo-inosose synthase

Crystallization

Crystallization on EC 4.2.3.124 - 2-deoxy-scyllo-inosose synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified free enzyme and enzyme in complex with substrate analogue inhibitor carbaglucose 6-phosphate, and with NAD+, and Co2+, hanging drop vapour diffusion method, mixing of 0.002 ml pf 4.2 mg/ml protein in 5 mM Tris-HCl, pH 7.7, and 0.2 mM CoCl2, with 0.002 ml of reservoir solution containing 40% w/v PEG 4000, 200 mM Li2SO4, and 100 mM Tris-HCl, pH 8.6, equilibration against 1 ml of reservoir solution, addition of 1 mM Co2+ and 1 mM inhibitor, 4°C, X-ray diffraction structure determination and analysis at 2.15-2.3 A resoltuion, heavy atom derivatization
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.002 ml pf 4.2 mg/ml protein in 5 mM Tris-HCl, pH 7.7, and 0.2 mM CoCl2, with 0.002 ml of reservoir solution containing 40% w/v PEG 4000, 200 mM Li2SO4, and 100 mM Tris-HCl, pH 8.6, equilibration against 1 ml of reservoir solution, 4°C, X-ray diffraction structure determination and analysis at 2.3 A resolution