Cloned (Comment) | Organism |
---|---|
gene btrC, recombinant expression | Niallia circulans |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.002 ml pf 4.2 mg/ml protein in 5 mM Tris-HCl, pH 7.7, and 0.2 mM CoCl2, with 0.002 ml of reservoir solution containing 40% w/v PEG 4000, 200 mM Li2SO4, and 100 mM Tris-HCl, pH 8.6, equilibration against 1 ml of reservoir solution, 4°C, X-ray diffraction structure determination and analysis at 2.3 A resolution | Niallia circulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | Q9S5E2 | gene btrC | - |
Niallia circulans SANK72073 | Q9S5E2 | gene btrC | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme by gel filtration, ultracentrifugation, and hydrophobic interaction chromatography | Niallia circulans |
Synonyms | Comment | Organism |
---|---|---|
btrC | - |
Niallia circulans |
DOIS | - |
Niallia circulans |
General Information | Comment | Organism |
---|---|---|
metabolism | key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. Multistep cyclization of D-glucose-6-phosphate into the six-membered carbocycle 2-deoxy-L-scyllo-inosose is the first step of 2-deoxystreptamine biosynthesis | Niallia circulans |