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Literature summary for 4.2.3.124 extracted from

  • Nango, E.; Kumasaka, T.; Sato, T.; Tanaka, N.; Kakinuma, K.; Eguchi, T.
    Crystallization and X-ray analysis of 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics (2005), Acta Crystallogr. Sect. F, 61, 709-711.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene btrC, recombinant expression Niallia circulans

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.002 ml pf 4.2 mg/ml protein in 5 mM Tris-HCl, pH 7.7, and 0.2 mM CoCl2, with 0.002 ml of reservoir solution containing 40% w/v PEG 4000, 200 mM Li2SO4, and 100 mM Tris-HCl, pH 8.6, equilibration against 1 ml of reservoir solution, 4°C, X-ray diffraction structure determination and analysis at 2.3 A resolution Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans Q9S5E2 gene btrC
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Niallia circulans SANK72073 Q9S5E2 gene btrC
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Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme by gel filtration, ultracentrifugation, and hydrophobic interaction chromatography Niallia circulans

Synonyms

Synonyms Comment Organism
btrC
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Niallia circulans
DOIS
-
Niallia circulans

General Information

General Information Comment Organism
metabolism key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. Multistep cyclization of D-glucose-6-phosphate into the six-membered carbocycle 2-deoxy-L-scyllo-inosose is the first step of 2-deoxystreptamine biosynthesis Niallia circulans