Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
reaction mechanism, overview
-
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
reaction mechanism, overview
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
reaction mechanism, overview
-
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
reaction mechanism, overview
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
catalyric reaction mechanism and substrate specificity, overview
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
DOIS catalyzes the multi-step and direct cyclization reaction of G-6-P consisting five crucial transformations, which include the initial NAD+-dependent dehydrogenation at C-4, the elimination of a phosphate from C-5 and C-6, the hydride comeback to C-4 to form a cyclic enol ether, and the finial aldol-type intramolecular condensation between C-6 and C-1
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
reaction and stereochemical mechanisms, overview
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
reaction mechanism of the multistep reaction, stereochemistry of the phosphate elimination reaction, proceeding through syn-elimination assisted by Glu 243, and the cyclization, an aldol condensation that proceeds via a boat conformation, overview
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
the reaction mechanism includes oxidation of C-4, beta-elimination of phosphate, reduction of C-4, ring opening, and intramolecular aldol cyclization. Completely conserved E243 of BtrC is catalytically involved in the phosphate elimination step
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
reaction mechanism, overview
-
-
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
catalyric reaction mechanism and substrate specificity, overview
-
-
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
reaction mechanism, overview
-
-
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate
reaction mechanism of the multistep reaction, stereochemistry of the phosphate elimination reaction, proceeding through syn-elimination assisted by Glu 243, and the cyclization, an aldol condensation that proceeds via a boat conformation, overview
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-deoxy-D-glucose 6-phosphate
2,5-dideoxy-L-scyllo-inosose + phosphate
3-deoxy-D-glucose 6-phosphate
2,4-dideoxy-L-scyllo-inosose + phosphate
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
additional information
?
-
2-deoxy-D-glucose 6-phosphate
2,5-dideoxy-L-scyllo-inosose + phosphate
low activity
-
-
?
2-deoxy-D-glucose 6-phosphate
2,5-dideoxy-L-scyllo-inosose + phosphate
low activity
-
-
?
3-deoxy-D-glucose 6-phosphate
2,4-dideoxy-L-scyllo-inosose + phosphate
low activity
-
-
?
3-deoxy-D-glucose 6-phosphate
2,4-dideoxy-L-scyllo-inosose + phosphate
low activity
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
carbocycle formation
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization, mechanism, overview
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization, multistep process
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
multistep reaction
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
the enzyme converts ubiquitous D-glucose 6-phosphate into the specific carbocycle, 2-deoxy-scyllo-inosose with an aid of NAD+-NADH recycling
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
cyclization stereochemistry, overview
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization, hydride abstraction and returning appeared to take place within the same glucose molecule
product identification by GC-MS analysis
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
pro-R stereospecific hydride transfer by NAD+-catalyzed 2-deoxy-scyllo-inosose synthase
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
carbocycle formation
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
multistep reaction
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization, mechanism, overview
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization, hydride abstraction and returning appeared to take place within the same glucose molecule
product identification by GC-MS analysis
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization, multistep process
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
intramolecular carbocyclization, multistep process
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
intramolecular carbocyclization, multistep process
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
additional information
?
-
the hydroxy group at C-4 is essential for enzyme activity, while the hydroxy groups at C-2 and C-3 are significant for efficial transformation of natural glucose 6-phosphate, but not essential for the reaction catalysis at the active site of enzyme, no activity with 4-deoxy-glucose 6-phosphate, substrate specificity, overview
-
-
?
additional information
?
-
specific hydrogen-bond accepting and donating interactions between the hydroxy groups at C-2 and C-3 of the substrate and the enzyme are responsible for the recognition
-
-
?
additional information
?
-
substrate specificity of 2-deoxy-scyllo-inosose synthase towards deoxyglucose 6-phosphates and proposed mechanism
-
-
?
additional information
?
-
the hydroxy group at C-4 is essential for enzyme activity, while the hydroxy groups at C-2 and C-3 are significant for efficial transformation of natural glucose 6-phosphate, but not essential for the reaction catalysis at the active site of enzyme, no activity with 4-deoxy-glucose 6-phosphate, substrate specificity, overview
-
-
?
additional information
?
-
substrate specificity of 2-deoxy-scyllo-inosose synthase towards deoxyglucose 6-phosphates and proposed mechanism
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
additional information
?
-
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
carbocycle formation
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization, mechanism, overview
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization, multistep process
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
multistep reaction
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
the enzyme converts ubiquitous D-glucose 6-phosphate into the specific carbocycle, 2-deoxy-scyllo-inosose with an aid of NAD+-NADH recycling
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
carbocycle formation
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
multistep reaction
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization, mechanism, overview
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
intramolecular carbocyclization, multistep process
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
intramolecular carbocyclization, multistep process
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
intramolecular carbocyclization, multistep process
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
-
-
-
-
?
additional information
?
-
the hydroxy group at C-4 is essential for enzyme activity, while the hydroxy groups at C-2 and C-3 are significant for efficial transformation of natural glucose 6-phosphate, but not essential for the reaction catalysis at the active site of enzyme, no activity with 4-deoxy-glucose 6-phosphate, substrate specificity, overview
-
-
?
additional information
?
-
the hydroxy group at C-4 is essential for enzyme activity, while the hydroxy groups at C-2 and C-3 are significant for efficial transformation of natural glucose 6-phosphate, but not essential for the reaction catalysis at the active site of enzyme, no activity with 4-deoxy-glucose 6-phosphate, substrate specificity, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
evolution
2-deoxy-scyllo-inosose synthase, i.e. DOIS or BtrC, clearly belongs to the family of dehydroquinate synthase, DHQS
evolution
evolutionary relationship between dehydroquinate synthase and 2-deoxy-scyllo-inosose synthase, overview
malfunction
disruption of gene btrC2 reduces the growth rate and antibiotics production, the growth rate is restored by addition of NH4Cl, both by addition of yeast extract
malfunction
-
disruption of gene btrC2 reduces the growth rate and antibiotics production, the growth rate is restored by addition of NH4Cl, both by addition of yeast extract
-
metabolism
2-deoxy-scyllo-inosose synthase is the starter enzyme for 2-deoxystreptamine biosynthesis. 2-Deoxystreptamine is the aglycon of clinically important aminocyclitol antibiotics
metabolism
2-deoxy-scyllo-inosose synthase, DOIS, is a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics
metabolism
-
DOI synthase is a key enzyme in the biosynthesis of 2-deoxy-scyllo-inosose, DOS
metabolism
-
DOI synthase is a key enzyme in the biosynthesis of 2-deoxy-scyllo-inosose, DOS
metabolism
-
DOI synthase is a key enzyme in the biosynthesis of 2-deoxy-scyllo-inosose, DOS
metabolism
DOI synthase is the key starter enzyme of 2-deoxystreptamine biosynthesis
metabolism
key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics
metabolism
key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. Multistep cyclization of D-glucose-6-phosphate into the six-membered carbocycle 2-deoxy-L-scyllo-inosose is the first step of 2-deoxystreptamine biosynthesis
metabolism
key enzyme in the biosynthesis of 2-deoxytstreptamine. The function od BtrC2 in biosynthesis of butirosin is indirect. BtrC2 is involved in secondary as well as primary metabolism probably playing a role in stabilizing and regulating DOI synthase
metabolism
key enzyme in the biosynthesis of clinically important aminoglycoside antibiotics is 2-deoxy-scyllo-inosose synthase, which catalyzes carbocycle formation from D-glucose 6-phosphate to 2-deoxy-scyllo-inosose through a multistep reaction
metabolism
-
the enzyme is involved in the biosynthesis of another major group of classical aminocyclitol antibiotics which contain the 2-deoxystreptamine (DOS) moiety such as neomycin, kanamycin, tobramycin, gentamicin, sisomicin, butirosin, or ribostamycin
metabolism
the enzyme is involved in the biosynthesis of another major group of classical aminocyclitol antibiotics which contain the 2-deoxystreptamine, DOS, moiety such as neomycin, kanamycin, tobramycin, gentamicin, sisomicin, butirosin, or ribostamycin
metabolism
-
the enzyme is involved in the biosynthesis of another major group of classical aminocyclitol antibiotics which contain the 2-deoxystreptamine, DOS, moiety such as neomycin, kanamycin, tobramycin, gentamicin, sisomicin, butirosin, or ribostamycin
metabolism
-
DOI synthase is a key enzyme in the biosynthesis of 2-deoxy-scyllo-inosose, DOS
-
metabolism
-
the enzyme is involved in the biosynthesis of another major group of classical aminocyclitol antibiotics which contain the 2-deoxystreptamine (DOS) moiety such as neomycin, kanamycin, tobramycin, gentamicin, sisomicin, butirosin, or ribostamycin
-
metabolism
-
DOI synthase is a key enzyme in the biosynthesis of 2-deoxy-scyllo-inosose, DOS
-
metabolism
-
DOI synthase is a key enzyme in the biosynthesis of 2-deoxy-scyllo-inosose, DOS
-
metabolism
-
2-deoxy-scyllo-inosose synthase is the starter enzyme for 2-deoxystreptamine biosynthesis. 2-Deoxystreptamine is the aglycon of clinically important aminocyclitol antibiotics
-
metabolism
-
the enzyme is involved in the biosynthesis of another major group of classical aminocyclitol antibiotics which contain the 2-deoxystreptamine, DOS, moiety such as neomycin, kanamycin, tobramycin, gentamicin, sisomicin, butirosin, or ribostamycin
-
metabolism
-
key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. Multistep cyclization of D-glucose-6-phosphate into the six-membered carbocycle 2-deoxy-L-scyllo-inosose is the first step of 2-deoxystreptamine biosynthesis
-
metabolism
-
2-deoxy-scyllo-inosose synthase, DOIS, is a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics
-
metabolism
-
key enzyme in the biosynthesis of clinically important aminoglycoside antibiotics is 2-deoxy-scyllo-inosose synthase, which catalyzes carbocycle formation from D-glucose 6-phosphate to 2-deoxy-scyllo-inosose through a multistep reaction
-
metabolism
-
key enzyme in the biosynthesis of 2-deoxytstreptamine. The function od BtrC2 in biosynthesis of butirosin is indirect. BtrC2 is involved in secondary as well as primary metabolism probably playing a role in stabilizing and regulating DOI synthase
-
physiological function
2-deoxy-scyllo-inosose synthase is involved in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics and catalyzes the carbocyclic formation from D-glucose 6-phosphate into 2-deoxy-scyllo-inosose
physiological function
-
the DOI synthase reaction is a crucial starter step for the biosynthetic pathway of DOS-containing aminocyclitol antibiotics. The DOI synthase catalyzes the intramolecular carbocyclization of D-glucose 6-phosphate into the first non-aminogenous cyclitol 2-deoxy-L-scyllo-inosose, DOI
physiological function
the DOI synthase reaction is a crucial starter step for the biosynthetic pathway of DOS-containing aminocyclitol antibiotics. The DOI synthase catalyzes the intramolecular carbocyclization of D-glucose 6-phosphate into the first non-aminogenous cyclitol 2-deoxy-L-scyllo-inosose, DOI
physiological function
the enzyme is crucial in the carbocyclization reaction as part of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. The DOI synthase catalyzes the intramolecular carbocyclization of D-glucose 6-phosphate into the first non-aminogenous cyclitol 2-deoxy-L-scyllo-inosose, DOI
physiological function
-
the enzyme is crucial in the carbocyclization reaction as part of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. The DOI synthase catalyzes the intramolecular carbocyclization of D-glucose 6-phosphate into the first non-aminogenous cyclitol 2-deoxy-L-scyllo-inosose, DOI
physiological function
the role of the 20 kDa non-catalytic protein BtrC2, associated with the DOI synthase, in butirosin biosynthesis is to stabilize the synthase by complexing as heterodimer with BtrC allowing the stable production of butirosin for long periods. BtrC2 is also involved in the biosynthesis of vitamin B6 in primary metabolism
physiological function
2-deoxy-scyllo-inosose (DOI) synthase, which uses glucose-6-phosphate as a substrate for DOI biosynthesis, is indispensably involved in the initial stage of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics including butirosin, gentamicin, kanamycin, and tobramycin
physiological function
2-deoxy-scyllo-inosose (DOI) synthase, which uses glucose-6-phosphate as a substrate for DOI biosynthesis, is indispensably involved in the initial stage of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics including butirosin, gentamicin, kanamycin, and tobramycin
physiological function
DOI synthase (DOIS) is responsible for the formation of DOI from D-glucose-6-phosphate in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics such as neomycin and butirosin
physiological function
-
2-deoxy-scyllo-inosose (DOI) synthase, which uses glucose-6-phosphate as a substrate for DOI biosynthesis, is indispensably involved in the initial stage of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics including butirosin, gentamicin, kanamycin, and tobramycin
-
physiological function
-
the DOI synthase reaction is a crucial starter step for the biosynthetic pathway of DOS-containing aminocyclitol antibiotics. The DOI synthase catalyzes the intramolecular carbocyclization of D-glucose 6-phosphate into the first non-aminogenous cyclitol 2-deoxy-L-scyllo-inosose, DOI
-
physiological function
-
the enzyme is crucial in the carbocyclization reaction as part of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. The DOI synthase catalyzes the intramolecular carbocyclization of D-glucose 6-phosphate into the first non-aminogenous cyclitol 2-deoxy-L-scyllo-inosose, DOI
-
physiological function
-
the DOI synthase reaction is a crucial starter step for the biosynthetic pathway of DOS-containing aminocyclitol antibiotics. The DOI synthase catalyzes the intramolecular carbocyclization of D-glucose 6-phosphate into the first non-aminogenous cyclitol 2-deoxy-L-scyllo-inosose, DOI
-
physiological function
-
the role of the 20 kDa non-catalytic protein BtrC2, associated with the DOI synthase, in butirosin biosynthesis is to stabilize the synthase by complexing as heterodimer with BtrC allowing the stable production of butirosin for long periods. BtrC2 is also involved in the biosynthesis of vitamin B6 in primary metabolism
-
additional information
active site structure is located between the N-terminal and C-terminal domains. Two subunits exist as a dimer in the asymmetric unit. The two active sites of the dimer are different. One contains a dephosphorylated compound derived from the inhibitor and the other includes the inhibitor without change. Comparison of active site between the native and complex BtrC, overview
additional information
-
active site structure is located between the N-terminal and C-terminal domains. Two subunits exist as a dimer in the asymmetric unit. The two active sites of the dimer are different. One contains a dephosphorylated compound derived from the inhibitor and the other includes the inhibitor without change. Comparison of active site between the native and complex BtrC, overview
additional information
-
Streptoalloteichus hindustanus seems to have two sets of DOS-containing antibiotic biosynthetic gene cluster for tobramycin and apramycin
additional information
the characteristic glutamate residue E243 of DOI synthase is a key determinant to distinguish the reaction mechanism between DOI synthase and dehydroquinate, DHQ, synthase as well as primary sequence, Substrate recognition models of DOI synthase and DHQ synthase, overview
additional information
-
the characteristic glutamate residue E243 of DOI synthase is a key determinant to distinguish the reaction mechanism between DOI synthase and dehydroquinate, DHQ, synthase as well as primary sequence, Substrate recognition models of DOI synthase and DHQ synthase, overview
additional information
while the reaction mechanisms are quite similar, 2-deoxy-scyllo-inosose synthase is distinct from 3-dehydroquinate synthase, EC 4.2.3.4, in the shikimate pathway, with respect to the quaternary structure, metal ion requirement, and the kinetic parameters
additional information
-
while the reaction mechanisms are quite similar, 2-deoxy-scyllo-inosose synthase is distinct from 3-dehydroquinate synthase, EC 4.2.3.4, in the shikimate pathway, with respect to the quaternary structure, metal ion requirement, and the kinetic parameters
additional information
-
Streptoalloteichus hindustanus seems to have two sets of DOS-containing antibiotic biosynthetic gene cluster for tobramycin and apramycin
-
additional information
-
while the reaction mechanisms are quite similar, 2-deoxy-scyllo-inosose synthase is distinct from 3-dehydroquinate synthase, EC 4.2.3.4, in the shikimate pathway, with respect to the quaternary structure, metal ion requirement, and the kinetic parameters
-
additional information
-
active site structure is located between the N-terminal and C-terminal domains. Two subunits exist as a dimer in the asymmetric unit. The two active sites of the dimer are different. One contains a dephosphorylated compound derived from the inhibitor and the other includes the inhibitor without change. Comparison of active site between the native and complex BtrC, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
E243Q
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
K141Q
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
additional information
complementation of a btrC2 disruption mutant by expression of wild-type btrC2 from plasmid pHB201 in Bacillus circulans
additional information
-
complementation of a btrC2 disruption mutant by expression of wild-type btrC2 from plasmid pHB201 in Bacillus circulans
additional information
disruption of gene btrC2 by insertion of a tetracycline resistance cassette
additional information
-
disruption of gene btrC2 by insertion of a tetracycline resistance cassette
additional information
introduction of DOIS gene btrC from Bacillus circulans and disruption of genes for phosphoglucose isomerase, D-glucose 6-phosphate dehydrogenase, and phosphoglucomutase, pgi, zwf and pgm, respectively, in Escherichia coli strain GI724. While GI724DELTAP/pGA-btrC and GI724DELTAPP/pGA-btrC possess functional Zwf and thus can catabolize glucose via the pentose phosphate pathway, glucose catabolism is completely inhibited in GI724DELTAPZ/pGA-btrC and GI724DELTAPZP/pGA-btrC due to the deletion of both pgi and zwf genes
additional information
-
introduction of DOIS gene btrC from Bacillus circulans and disruption of genes for phosphoglucose isomerase, D-glucose 6-phosphate dehydrogenase, and phosphoglucomutase, pgi, zwf and pgm, respectively, in Escherichia coli strain GI724. While GI724DELTAP/pGA-btrC and GI724DELTAPP/pGA-btrC possess functional Zwf and thus can catabolize glucose via the pentose phosphate pathway, glucose catabolism is completely inhibited in GI724DELTAPZ/pGA-btrC and GI724DELTAPZP/pGA-btrC due to the deletion of both pgi and zwf genes
additional information
construction of several metabolically engineered recombinant strains of Bacillus subtilis, and comparison of the DOI titer produced by the recombinants that express a heterologous DOI synthase. The expression of a natural btrC gene, encoding DOI synthase in butirosin-producing Bacillus circulans, in the heterologous host Bacillus subtilis strain 168-BSDOI-2 generates approximately 2.3 g/l 2-deoxy-scyllo-inosose (DOI), fed-batch fermentation, UPLC-ESI-MS/MS analysis of DOI production
additional information
transgenic production of 2-deoxy-scyllo-inosose (DOI) in cyanobacterium Synechococcus elongatus strain PCC 7942, production of 400 mg/l of DOI without using an external carbon source, method overview
additional information
-
transgenic production of 2-deoxy-scyllo-inosose (DOI) in cyanobacterium Synechococcus elongatus strain PCC 7942, production of 400 mg/l of DOI without using an external carbon source, method overview
additional information
-
introduction of DOIS gene btrC from Bacillus circulans and disruption of genes for phosphoglucose isomerase, D-glucose 6-phosphate dehydrogenase, and phosphoglucomutase, pgi, zwf and pgm, respectively, in Escherichia coli strain GI724. While GI724DELTAP/pGA-btrC and GI724DELTAPP/pGA-btrC possess functional Zwf and thus can catabolize glucose via the pentose phosphate pathway, glucose catabolism is completely inhibited in GI724DELTAPZ/pGA-btrC and GI724DELTAPZP/pGA-btrC due to the deletion of both pgi and zwf genes
-
additional information
-
complementation of a btrC2 disruption mutant by expression of wild-type btrC2 from plasmid pHB201 in Bacillus circulans
-
additional information
-
disruption of gene btrC2 by insertion of a tetracycline resistance cassette
-
additional information
construction of several metabolically engineered recombinant strains of Bacillus subtilis, and comparison of the DOI titer produced by the recombinants that express a heterologous DOI synthase. The expression of gene btrC encoding DOI synthase derived from tobramycin-producing Streptomyces tenebrarius in recombinant of Bacillus subtilis strain BSDOI-15, in which both genes pgi and pgcA are disrupted, significantly enhances the 2-deoxy-scyllo-inosose (DOI) titer to up to 37.2 g/l, fed-batch fermentation, UPLC-ESI-MS/MS analysis of DOI production
additional information
-
construction of several metabolically engineered recombinant strains of Bacillus subtilis, and comparison of the DOI titer produced by the recombinants that express a heterologous DOI synthase. The expression of gene btrC encoding DOI synthase derived from tobramycin-producing Streptomyces tenebrarius in recombinant of Bacillus subtilis strain BSDOI-15, in which both genes pgi and pgcA are disrupted, significantly enhances the 2-deoxy-scyllo-inosose (DOI) titer to up to 37.2 g/l, fed-batch fermentation, UPLC-ESI-MS/MS analysis of DOI production
additional information
-
construction of several metabolically engineered recombinant strains of Bacillus subtilis, and comparison of the DOI titer produced by the recombinants that express a heterologous DOI synthase. The expression of gene btrC encoding DOI synthase derived from tobramycin-producing Streptomyces tenebrarius in recombinant of Bacillus subtilis strain BSDOI-15, in which both genes pgi and pgcA are disrupted, significantly enhances the 2-deoxy-scyllo-inosose (DOI) titer to up to 37.2 g/l, fed-batch fermentation, UPLC-ESI-MS/MS analysis of DOI production
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
complementation of a btrC2 disruption mutant by expression of wild-type btrC2 from plasmid pHB201 in Bacillus circulans
gene alloH, DNA and amino acid sequence determination and analysis, comparisons of the gene cluster with similar ones, expression in Escherichia coli
-
gene brtC, functional recombinant expression in Bacillus subtilis strain BSDOI-2, and functional recombinant expression from codo-optimzed gene in Streptomyces tenebrarius
gene btrC, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain JM 105, expression in Escherichia coli strain BL21(DE3)
-
gene btrC, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain JM 105, functional expression in Escherichia coli strain BL21(DE3)
gene btrC, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Escherichia coli strain BL21(DE3)
gene btrC, expression in Escherichia coli
gene btrC, functional recombinant expression in Synechococcus elongatus strain PCC 7942
gene btrC, recombinant expression
gene DOIS, expression in Escherichia coli strain GI724 from plasmid pGA-btrC
gene kanA, expression in Escherichia coli strains XL1-Blue MRF and BL21 (DE3), in the latter as His6-tagged protein, using plasmid pIBR25, formation of insoluble KanA, expression in Streptomyces lividans strain TK24 using pIBR25 with a MCS is successful
-
gene tbmA, functional recombinant expression codon-optimized gene in Bacillus subtilis strain 168-DOI-15, in which both genes pgi and pgcA are disrupted
genes btrC and btrC2, co-overexpression in Escherichia coli strain JM109
gene btrC, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Escherichia coli strain BL21(DE3)
-
gene btrC, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Escherichia coli strain BL21(DE3)
-
gene btrC, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Escherichia coli strain BL21(DE3)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Nango, E.; Kumasaka, T.; Sato, T.; Tanaka, N.; Kakinuma, K.; Eguchi, T.
Crystallization and X-ray analysis of 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics
Acta Crystallogr. Sect. F
61
709-711
2005
Niallia circulans (Q9S5E2), Niallia circulans, Niallia circulans SANK72073 (Q9S5E2)
brenda
Huang, Z.; Kakinuma, K.; Eguchi, T.
Stereospecificity of hydride transfer in NAD+-catalyzed 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics
Bioorg. Chem.
33
82-89
2005
Niallia circulans (Q9S5E2)
brenda
Hirayama, T.; Kudo, F.; Huang, Z.; Eguchi, T.
Role of glutamate 243 in the active site of 2-deoxy-scyllo-inosose synthase from Bacillus circulans
Bioorg. Med. Chem.
15
418-423
2007
Niallia circulans (Q9S5E2), Niallia circulans
brenda
Iwase, N.; Kudo, F.; Yamauchi, N.; Kakinuma, K.
Substrate specificity of 2-deoxy-scyllo-inosose synthase, the starter enzyme for 2-deoxystreptamine biosynthesis, toward deoxyglucose-6-phosphates and proposed mechanism
Biosci. Biotechnol. Biochem.
62
2396-2407
1998
Niallia circulans (Q9S5E2), Niallia circulans SANK72073 (Q9S5E2)
brenda
Tamegai, H.; Nango, E.; Koike-Takeshita, A.; Kudo, F.; Kakinuma, K.
Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo-inosose synthase for the biosynthesis of butirosin antibiotics in Bacillus circulans
Biosci. Biotechnol. Biochem.
66
1538-1545
2002
Niallia circulans (Q9S5E2), Niallia circulans, Niallia circulans SANK72073 (Q9S5E2)
brenda
Tamegai, H.; Sawada, H.; Nango, E.; Aoki, R.; Hirakawa, H.; Iino, T.; Eguchi, T.
Roles of a 20 kDa protein associated with a carbocycle-forming enzyme involved in aminoglycoside biosynthesis in primary and secondary metabolism
Biosci. Biotechnol. Biochem.
74
1215-1219
2010
Niallia circulans (Q9S5E2), Niallia circulans, Niallia circulans SANK72073 (Q9S5E2)
brenda
Thuy, M.L.; Kharel, M.K.; Lamichhane, R.; Lee, H.C.; Suh, J.W.; Liou, K.; Sohng, J.K.
Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans
Biotechnol. Lett.
27
465-470
2005
Streptomyces lividans
brenda
Nango, E.; Kudo, F.; Eguchi, T.; Kakinuma, K.
Reaction stereochemistry of 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine
Chem. Lett.
32
438-439
2003
Niallia circulans (Q9S5E2)
-
brenda
Kudo, F.; Yamauchi, N.; Suzuki, R.; Kakinuma, K.
Kinetic isotope effect and reaction mechanism of 2-deoxy-scyllo-inosose synthase derived from butirosin-producing Bacillus circulans
J. Antibiot.
50
424-428
1997
Niallia circulans (Q9S5E2), Niallia circulans, Niallia circulans SANK72073 (Q9S5E2)
brenda
Kudo, F.; Tamegai, H.; Fujiwara, T.; Tagami, U.; Hirayama, K.; Kakinuma, K.
Molecular cloning of the gene for the key carbocycle-forming enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics and its comparison with dehydroquinate synthase
J. Antibiot.
52
559-571
1999
Streptomyces fradiae, Niallia circulans (Q9S5E2), Niallia circulans, Streptomyces fradiae IFO 13147, Niallia circulans SANK72073 (Q9S5E2)
brenda
Kudo, F.; Hosomi, Y.; Tamegai, H.; Kakinuma, K.
Purification and characterization of 2-deoxy-scyllo-inosose synthase derived from Bacillus circulans. A crucial carbocyclization enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics
J. Antibiot.
52
81-88
1999
Niallia circulans (Q9S5E2), Niallia circulans, Niallia circulans SANK72073 (Q9S5E2)
brenda
Hirayama, T.; Tamegai, H.; Kudo, F.; Kojima, K.; Kakinuma, K.; Eguchi, T.
Biosynthesis of 2-deoxystreptamine-containing antibiotics in Streptoalloteichus hindustanus JCM 3268: characterization of 2-deoxy-scyllo-inosose synthase
J. Antibiot.
59
358-361
2006
Streptoalloteichus hindustanus, Streptoalloteichus hindustanus JCM 3268
brenda
Kogure, T.; Wakisaka, N.; Takaku, H.; Takagi, M.
Efficient production of 2-deoxy-scyllo-inosose from D-glucose by metabolically engineered recombinant Escherichia coli
J. Biotechnol.
129
502-509
2007
Niallia circulans (Q9S5E2), Niallia circulans, Niallia circulans SANK72073 (Q9S5E2)
brenda
Kharel, M.; Subba, B.; Lee, H.; Liou, K.; Woo, J.; Kim, D.; Moon, Y.; Sohng, J.
Identification of 2-deoxy-scyllo-inosose synthase in aminoglycoside producer Streptomyces
J. Microbiol. Biotechnol.
13
828-831
2003
no activity in Streptomyces lividans, Streptomyces kanamyceticus, Streptomyces ribosidificus, Streptoalloteichus tenebrarius, Streptoalloteichus tenebrarius IFO 13396, Streptomyces kanamyceticus IFO13414, no activity in Streptomyces lividans TK24, Streptomyces ribosidificus IFO13796
-
brenda
Nango, E.; Eguchi, T.; Kakinuma, K.
Active site mapping of 2-deoxy-scyllo-inosose synthase, the key starter enzyme for the biosynthesis of 2-deoxystreptamine. Mechanism-based inhibition and identification of lysine-141 as the entrapped nucleophile
J. Org. Chem.
69
593-600
2004
Niallia circulans (Q9S5E2), Niallia circulans SANK72073 (Q9S5E2)
brenda
Nango, E.; Kumasaka, T.; Hirayama, T.; Tanaka, N.; Eguchi, T.
Structure of 2-deoxy-scyllo-inosose synthase, a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics, in complex with a mechanism-based inhibitor and NAD+
Proteins
70
517-527
2008
Niallia circulans (Q9S5E2), Niallia circulans, Niallia circulans SANK72073 (Q9S5E2)
brenda
Watanabe, S.; Ozawa, H.; Kato, H.; Nimura-Matsune, K.; Hirayama, T.; Kudo, F.; Eguchi, T.; Kakinuma, K.; Yoshikawa, H.
Carbon-free production of 2-deoxy-scyllo-inosose (DOI) in cyanobacterium Synechococcus elongatus PCC 7942
Biosci. Biotechnol. Biochem.
82
161-165
2018
Niallia circulans (Q9S5E2), Niallia circulans
brenda
Lim, J.H.; Hwang, H.H.; Lee, N.J.; Lee, J.W.; Seo, E.G.; Son, H.B.; Kim, H.J.; Yoon, Y.J.; Park, J.W.
Enhanced biosynthesis of 2-deoxy-scyllo-inosose in metabolically engineered Bacillus subtilis recombinants
Front. Microbiol.
9
2333
2018
Streptoalloteichus tenebrarius (Q2MF16), Streptoalloteichus tenebrarius, Niallia circulans (Q9S5E2), Streptoalloteichus tenebrarius ATCC 17920 (Q2MF16)
brenda