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Literature summary for 4.2.3.124 extracted from

  • Hirayama, T.; Tamegai, H.; Kudo, F.; Kojima, K.; Kakinuma, K.; Eguchi, T.
    Biosynthesis of 2-deoxystreptamine-containing antibiotics in Streptoalloteichus hindustanus JCM 3268: characterization of 2-deoxy-scyllo-inosose synthase (2006), J. Antibiot., 59, 358-361.
    View publication on PubMed
Search for more literature for 4.2.3.124

Cloned(Commentary)

Cloned (Comment) Organism
gene alloH, DNA and amino acid sequence determination and analysis, comparisons of the gene cluster with similar ones, expression in Escherichia coli Streptoalloteichus hindustanus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.69
-
 D-glucose 6-phosphate pH not specified in the publication, 46°C Streptoalloteichus hindustanus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate Streptoalloteichus hindustanus
-
 2-deoxy-L-scyllo-inosose + phosphate
-
 ?
D-glucose 6-phosphate Streptoalloteichus hindustanus JCM 3268
-
 2-deoxy-L-scyllo-inosose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptoalloteichus hindustanus
-
 gene allloH
-
Streptoalloteichus hindustanus JCM 3268
-
 gene allloH
-

Purification (Commentary)

Purification (Comment) Organism
recombinant AlloH from Escherichia coli by anion exchange chromatography and gel filtration Streptoalloteichus hindustanus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate
-
 Streptoalloteichus hindustanus 2-deoxy-L-scyllo-inosose + phosphate
-
 ?
D-glucose 6-phosphate
-
 Streptoalloteichus hindustanus JCM 3268 2-deoxy-L-scyllo-inosose + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
AlloH
-
 Streptoalloteichus hindustanus
DOI synthase
-
 Streptoalloteichus hindustanus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
46
-
 assay at Streptoalloteichus hindustanus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.075
-
 D-glucose 6-phosphate pH not specified in the publication, 46°C Streptoalloteichus hindustanus

Cofactor

Cofactor Comment Organism Structure
NAD+ required Streptoalloteichus hindustanus

General Information

General Information Comment Organism
additional information Streptoalloteichus hindustanus seems to have two sets of DOS-containing antibiotic biosynthetic gene cluster for tobramycin and apramycin Streptoalloteichus hindustanus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.11
-
 D-glucose 6-phosphate pH not specified in the publication, 46°C Streptoalloteichus hindustanus