Cloned (Comment) | Organism |
---|---|
gene alloH, DNA and amino acid sequence determination and analysis, comparisons of the gene cluster with similar ones, expression in Escherichia coli | Streptoalloteichus hindustanus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.69 | - |
D-glucose 6-phosphate | pH not specified in the publication, 46°C | Streptoalloteichus hindustanus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | Streptoalloteichus hindustanus | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | Streptoalloteichus hindustanus JCM 3268 | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptoalloteichus hindustanus | - |
gene allloH | - |
Streptoalloteichus hindustanus JCM 3268 | - |
gene allloH | - |
Purification (Comment) | Organism |
---|---|
recombinant AlloH from Escherichia coli by anion exchange chromatography and gel filtration | Streptoalloteichus hindustanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | - |
Streptoalloteichus hindustanus | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | - |
Streptoalloteichus hindustanus JCM 3268 | 2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlloH | - |
Streptoalloteichus hindustanus |
DOI synthase | - |
Streptoalloteichus hindustanus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
assay at | Streptoalloteichus hindustanus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
D-glucose 6-phosphate | pH not specified in the publication, 46°C | Streptoalloteichus hindustanus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Streptoalloteichus hindustanus |
General Information | Comment | Organism |
---|---|---|
additional information | Streptoalloteichus hindustanus seems to have two sets of DOS-containing antibiotic biosynthetic gene cluster for tobramycin and apramycin | Streptoalloteichus hindustanus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
D-glucose 6-phosphate | pH not specified in the publication, 46°C | Streptoalloteichus hindustanus |