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4.2.2.1: hyaluronate lyase

This is an abbreviated version!
For detailed information about hyaluronate lyase, go to the full flat file.

Word Map on EC 4.2.2.1

Reaction

[hyaluronate]n
=
(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1
 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine +
hyaluronate

Synonyms

acidic hyase, bacterial HAse, bacterial hyaluronidase, bacterial spreading factor, bacteriophage-associated hyaluronate lyase, Cumulase, EC 4.2.99.1, GBS HA lyase, GBS hyase, glucuronoglycosaminoglycan lyase, h-SD, h-SH, HA lyase, HL, Hyal, Hyal-1, Hyal-2, HYAL1, Hyal2, HYAL3, hyaluronan lyase, hyaluronate lyase, hyaluronate lyase HylA, hyaluronate lyase HylP2, hyaluronidase, hyaluronidase 3, hyaluronidase SD, hyaluronidase SH, hyaluronidase-1, hyaluronidase-2, hyaluronoglucosaminidase-1, hyaluronoglucosaminidase-2, HYase, HylA7, hylB, hylB4755, HylP, Hylp2, LUCA-1, LUCA-2, lyase, glucuronoglycosaminoglycan, lyase, hyaluronate, More, mucinase, protein SEQ2045, rHuPH20, SagHL, SagHyal, spnHL, SpnHyal, spreading factor, SP_0314

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.1 hyaluronate lyase

Molecular Weight

Molecular Weight on EC 4.2.2.1 - hyaluronate lyase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
x * 100000, SDS-PAGE, x * 85000, SDS-PAGE, x * 75000-50000, trypsin digested enzyme, SDS-PAGE
107000
x * 107000, amino acid sequence calculation
110000
111000
-
pro-form, gel filtration
114000
x * 114000, amino acid sequence calculation
120000
recombinant enzyme, glutaraldehyde cross-linking
121000
x * 121000, amino acid sequence calculation
126000
-
x * 126000, SDS-PAGE
126500 - 130000
amino acid sequence calculation and native PAGE
39500
-
x * 39500, SDS-PAGE
40000
41000
x * 41000, N-terminal domain, SDS-PAGE, x * 41000, C-terminal domain, SDS-PAGE
44000
-
x * 44000 + x * 47000, HYAL3 in sperm, SDS-PAGE
47000
50000
-
x * 50000, SDS-PAGE
54260
x * 54260, sequence calculation
55000
-
x * 47000 + x * 55000, HYAL3 in sperm, SDS-PAGE
70000
-
gel filtration
70000 - 75000
-
gel filtration
80000
1 * 80000, SDS-PAGE
82000
x * 82000, amino acid sequence calculation
83027
x * 83208, mutant W292A, mass spectrometry, x * 83273, mutant F343V, mass spectrometry, x * 83097, mutant W291A/W292A, mass spectrometry, x * 83102, mutant W292A/F343V, mass spectrometry, x * 83027, mutant W291A/W292A/F343V, mass spectrometry
83097
x * 83208, mutant W292A, mass spectrometry, x * 83273, mutant F343V, mass spectrometry, x * 83097, mutant W291A/W292A, mass spectrometry, x * 83102, mutant W292A/F343V, mass spectrometry, x * 83027, mutant W291A/W292A/F343V, mass spectrometry
83102
x * 83208, mutant W292A, mass spectrometry, x * 83273, mutant F343V, mass spectrometry, x * 83097, mutant W291A/W292A, mass spectrometry, x * 83102, mutant W292A/F343V, mass spectrometry, x * 83027, mutant W291A/W292A/F343V, mass spectrometry
83208
x * 83208, mutant W292A, mass spectrometry, x * 83273, mutant F343V, mass spectrometry, x * 83097, mutant W291A/W292A, mass spectrometry, x * 83102, mutant W292A/F343V, mass spectrometry, x * 83027, mutant W291A/W292A/F343V, mass spectrometry
83218
x * 83218, calculation from nucleotide sequence
83273
x * 83208, mutant W292A, mass spectrometry, x * 83273, mutant F343V, mass spectrometry, x * 83097, mutant W291A/W292A, mass spectrometry, x * 83102, mutant W292A/F343V, mass spectrometry, x * 83027, mutant W291A/W292A/F343V, mass spectrometry
83280
mass spectrometry experiments
84000
85000
-
x * 100000, SDS-PAGE, x * 85000, SDS-PAGE, x * 75000-50000, trypsin digested enzyme, SDS-PAGE
85110
-
gel filtration
91000 - 107000
-
the recombinantly expressed enzyme exists in different proteolytically fragmented forms of the full-length enzyme with Mr of 107 kDa
92000