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4.2.2.1: hyaluronate lyase

This is an abbreviated version!
For detailed information about hyaluronate lyase, go to the full flat file.

Word Map on EC 4.2.2.1

Reaction

[hyaluronate]n
=
(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1
+ 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine +
hyaluronate

Synonyms

acidic hyase, bacterial HAse, bacterial hyaluronidase, bacterial spreading factor, bacteriophage-associated hyaluronate lyase, Cumulase, EC 4.2.99.1, GBS HA lyase, GBS hyase, glucuronoglycosaminoglycan lyase, h-SD, h-SH, HA lyase, HL, Hyal, Hyal-1, Hyal-2, HYAL1, Hyal2, HYAL3, hyaluronan lyase, hyaluronate lyase, hyaluronate lyase HylA, hyaluronate lyase HylP2, hyaluronidase, hyaluronidase 3, hyaluronidase SD, hyaluronidase SH, hyaluronidase-1, hyaluronidase-2, hyaluronoglucosaminidase-1, hyaluronoglucosaminidase-2, HYase, HylA7, hylB, hylB4755, HylP, Hylp2, LUCA-1, LUCA-2, lyase, glucuronoglycosaminoglycan, lyase, hyaluronate, More, mucinase, protein SEQ2045, rHuPH20, SagHL, SagHyal, spnHL, SpnHyal, spreading factor, SP_0314

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.1 hyaluronate lyase

Cloned

Cloned on EC 4.2.2.1 - hyaluronate lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
293 cells stably express Hyal-2
cloning overexpression, and purification of SagHL and its different fragments/domains
cloning, overexpression and purification of the C-terminal domain swapped chimeras of SpnHL and SagHL (hyaluronate lyase from Streptococcus agalactiae), modified enzyme SpnHLv/SagHLv (where the size of linker connecting the alpha-domain to C-terminal domain is enlarged by two amino acid)
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complementation of an enzyme-deficient mutant strain by expression of hysA
-
DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and truncation and point mutant enzymes in Escherichia coli strain BL21(DE3) using plasmid pSF49
DNA sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli
-
expressed in Escherichia coli TOP10
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
-
expression in Escherichia coli resulting in different protein sizes
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gene hyl, distribution, genetic diversity, and variable expression within the Streptococcus suis population, 309 isolates of different serotypes, geographic sources, and clinical backgrounds, overview
gene hyl, DNA and amino acid sequence determination and analysis, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene Hyl, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
His-tagged version expressed in Escherichia coli BL21(DE3)
Hyal-1/293 and Hyal-1/CD44/293 are stably overexpressing Hyal-1. The expressed Hyal-1 is not only accumulated intracellularly, but is also secreted to the outside of the cells
overexpression of fully functional truncated wild-type and mutant enzymes in Escherichia coli
-
overexpression of His-tagged enzyme in Escherichia coli
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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overexpression of mutant Y408F in Escherichia coli
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overexpression of the enzymes from strains 3502 and 4755 in Escherichia coli
overexpression of wild-type and mutant enzymes in Escherichia coli
-
overexpression of wild-type and mutant enzymes in Escherichia coli BL21(DE3)
recombinant expression of the C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)/pET30b-hylp
-