4.2.2.1: hyaluronate lyase This is an abbreviated version! For detailed information about hyaluronate lyase, go to the full flat file .
Reaction
[hyaluronate]n =
(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 +
2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
+
hyaluronate
Synonyms acidic hyase, bacterial HAse, bacterial hyaluronidase, bacterial spreading factor, bacteriophage-associated hyaluronate lyase, Cumulase, EC 4.2.99.1, GBS HA lyase, GBS hyase, glucuronoglycosaminoglycan lyase, h-SD, h-SH, HA lyase, HL, Hyal, Hyal-1, Hyal-2, HYAL1, Hyal2, HYAL3, hyaluronan lyase, hyaluronate lyase, hyaluronate lyase HylA, hyaluronate lyase HylP2, hyaluronidase, hyaluronidase 3, hyaluronidase SD, hyaluronidase SH, hyaluronidase-1, hyaluronidase-2, hyaluronoglucosaminidase-1, hyaluronoglucosaminidase-2, HYase, HylA7, hylB, hylB4755, HylP, Hylp2, LUCA-1, LUCA-2, lyase, glucuronoglycosaminoglycan, lyase, hyaluronate, More, mucinase, protein SEQ2045, rHuPH20, SagHL, SagHyal, spnHL, SpnHyal, spreading factor, SP_0314
ECTree
Cloned
Cloned on EC 4.2.2.1 - hyaluronate lyase
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293 cells stably express Hyal-2
cloning overexpression, and purification of SagHL and its different fragments/domains
cloning, overexpression and purification of the C-terminal domain swapped chimeras of SpnHL and SagHL (hyaluronate lyase from Streptococcus agalactiae), modified enzyme SpnHLv/SagHLv (where the size of linker connecting the alpha-domain to C-terminal domain is enlarged by two amino acid)
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complementation of an enzyme-deficient mutant strain by expression of hysA
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DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and truncation and point mutant enzymes in Escherichia coli strain BL21(DE3) using plasmid pSF49
DNA sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli
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expressed in Escherichia coli TOP10
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
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expression in Escherichia coli resulting in different protein sizes
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gene hyl, distribution, genetic diversity, and variable expression within the Streptococcus suis population, 309 isolates of different serotypes, geographic sources, and clinical backgrounds, overview
Q6H8W8 , Q6H8W9 , Q6H8X0 , Q6H8X1 , Q6H8X2 , Q6H8X4 , Q6H8X5 , Q6H8X6 , Q6H8X7 , Q6H8X8 , Q6H8Y0 , Q6H8Y1 , Q6H8Y2 , Q6H8Y3 , Q6H8Y4 , Q8VLQ7 , Q8VLQ8
gene hyl, DNA and amino acid sequence determination and analysis, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene Hyl, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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His-tagged version expressed in Escherichia coli BL21(DE3)
Hyal-1/293 and Hyal-1/CD44/293 are stably overexpressing Hyal-1. The expressed Hyal-1 is not only accumulated intracellularly, but is also secreted to the outside of the cells
overexpression of fully functional truncated wild-type and mutant enzymes in Escherichia coli
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overexpression of His-tagged enzyme in Escherichia coli
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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overexpression of mutant Y408F in Escherichia coli
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overexpression of the enzymes from strains 3502 and 4755 in Escherichia coli
overexpression of wild-type and mutant enzymes in Escherichia coli
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overexpression of wild-type and mutant enzymes in Escherichia coli BL21(DE3)
recombinant expression of the C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)/pET30b-hylp
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expressed in Escherichia coli TOP10
expressed in Escherichia coli TOP10
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expressed in Escherichia coli TOP10
expressed in Escherichia coli TOP10
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expressed in Escherichia coli TOP10
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expressed in Escherichia coli TOP10
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expressed in Escherichia coli TOP10
expressed in Escherichia coli TOP10
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expressed in Escherichia coli TOP10
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expressed in Escherichia coli TOP10
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expressed in Escherichia coli TOP10
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expressed in Escherichia coli TOP10
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expressed in Escherichia coli TOP10
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expressed in Escherichia coli TOP10
expression in Escherichia coli
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expression in Escherichia coli
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expression in Escherichia coli
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expression in Escherichia coli
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expression in Escherichia coli