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Literature summary for 4.2.2.1 extracted from
- Characterization of hyaluronate lyase from Streptococcus pyogenes bacteriophage H4489A (2011), Carbohydr. Polym., 84, 1182-1191.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of the C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)/pET30b-hylp | Streptococcus pyogenes phage H4489A |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | weak inhibition | Streptococcus pyogenes phage H4489A |  |
| Co2+ | complete inhibition | Streptococcus pyogenes phage H4489A | |
| Cu2+ | complete inhibition | Streptococcus pyogenes phage H4489A | |
| Ni2+ | complete inhibition | Streptococcus pyogenes phage H4489A | |
| SDS | complete inhibition | Streptococcus pyogenes phage H4489A | |
| Triton X-100 | weak inhibition | Streptococcus pyogenes phage H4489A | |
| Tween 80 | weak inhibition | Streptococcus pyogenes phage H4489A | |
| Zn2+ | strong inhibition | Streptococcus pyogenes phage H4489A | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.44 | - |
hyaluronate | pH 5.5, 37°C, recombinant enzyme | Streptococcus pyogenes phage H4489A | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | weak activation | Streptococcus pyogenes phage H4489A | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, about, recombinant His6-tagged enzyme, SDS-PAGE | Streptococcus pyogenes phage H4489A |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronate | Streptococcus pyogenes phage H4489A | efficient degradation of hyaluronate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pyogenes phage H4489A | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 53.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and cation exchange chromatography | Streptococcus pyogenes phage H4489A |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.62 | - |
pH 5.5, 37°C, purified recombinant enzyme | Streptococcus pyogenes phage H4489A |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronate | efficient degradation of hyaluronate | Streptococcus pyogenes phage H4489A | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, about, recombinant His6-tagged enzyme, SDS-PAGE | Streptococcus pyogenes phage H4489A |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HA lyase | - |
Streptococcus pyogenes phage H4489A |
| hyaluronate lyase | - |
Streptococcus pyogenes phage H4489A |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Streptococcus pyogenes phage H4489A |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | activity range, profile overview | Streptococcus pyogenes phage H4489A |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | purified recombinant enzyme, stable | Streptococcus pyogenes phage H4489A |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Streptococcus pyogenes phage H4489A |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | activity range, profile overview | Streptococcus pyogenes phage H4489A |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | purified recombinant enzyme, stable | Streptococcus pyogenes phage H4489A |
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